Histological facilities

We can offer training in use of the cryostat, microscope and staining procedures.

Technical data for our cryostat:

HM 560

Indication of data such as set and actual temperature, section sum, remaining travel, number of
sections, actual time, trim and fi ne sectioning thicknesses, retraction function
Automatic approach system (ACA)
Electronical handwheel brake – can be locked in any position
Separate temperature control for knife carrier down to -35°C
Temperature for specimen clamping +10°C to -50°C
Heated sliding window
Standby and sleep mode for saving energy
Automatic defrosting device with immediate defrosting and additional interruption function
Detach function for the fast detaching of specimens from the chucks
Heavy-duty rotary microtome
Section thicknesses from 0,5 µm to 100 µm:
 up to 2 µm in 0,5 µm-increments
 up to 10 µm in 1 µm-increments
 up to 20 µm in 2 µm-increments
 up to 50 µm in 5 µm-increments
 up to 100 µm in 10 µm-increments
Trimming section thicknesses from 5 µm to 500 µm:
 5 – 10 µm in 5 µm-increments
 10 – 100 µm in 10 µm-increments
 100 – 200 µm in 20 µm-increments
 200 – 500 µm in 50 µm-increments
Specimen retraction that can be turned off
Horizontal feed range: 48 mm
End position indication and automatic turn-off when reaching the front and rear end
positions of the horizontal feed.
Motorized coarse feed and reset movement with three different speed settings
Vertical cutting stroke: 60 mm
Max. specimen size: 70 x 55 mm
Specimen can be rotated in Z-axis, 360° and specimen orientation
0-positioning of the chuck
Cutting speed can be controlled continuously
Accelerated return travel for fast operation
Cutting window that can be selected
Optional
Three operating modes: interval, single and continuous stroke
Can be activated either via the foot pedal or manually
Motor brake and emergency stop switch for safe operation
Integrated Vacutome system for stretching cryosections as well as for the suction of debris
Integrated fast freezing device with separate cooling element (Peltier) down to max. -60°C

Facilities for advanced functional neuroanatomical studies

Confocal microscope:

  • Microscope stand Axio.Imager.Z1 (Carl Zeiss) with TFT monitor
  • Camera AxioCam MRc (Carl Zeiss)
  • Scanning stage 130x85 Piezo for upright stand
  • XY-stage controller WSB Piezodrive 05 with XY-Joystick for stage controller
  • HAL 100 illuminator with collector (transmitted light source) + Transmitted-light channel for LSM 5
  • X-Cite 120 fiber coupled illuminator (reflected-light source)
  • Laser sources:
    • Laser Module RGB (Argon laser 458, 477, 488, 514 nm; 30 mW)
    • DPSS laser 561 nm; 10 mW
    • HeHe laser 633 nm; 5 mW
    • Diode laser 405 nm; 30 mW
  • Scanning Module LSM 510 Meta
  • 2 x Ocular 10x/23 Brille
  • Objectives (all Carl Zeiss):
    • Plan Apochromat 10x/0.45 DIC II
    • Plan Apochromat 20x/0.8 DIC II
    • Plan Apochromat 40x/0.95 DIC II
    • Plan Apochromat 40x/1.3 Oil DIC II
    • Plan Apochromat 63x/1.4 Oil DIC II
    • Alpha Plan Apochromat 100x/1.46 Oil DIC II
  • Filtersets available(reflector turret) (all Carl Zeiss):
    • Filterset 00
    • Filterset 01
    • Filterset 09
    • Filterset 15
    • Filterset 20
    • Filterset 23
    • Filterset 24
    • Filterset 27
    • Filterset 28
    • Filterset DIC
  • Software: Carl Zeiss ZEN 2008 Version 5,0,0,267 with options for Physiology, FRET and FRAP.
  • Upcoming software update to ZEN 2009 (64 bit) in February 2014. 

MIRAX scanners

Automated scanning light microscope

  • 2 x MIRAX Midi BF/FL with power supply (24 V)
  • Both equipped with objective Plan Apochromat 20x/0.8
  • Camera adaptor 60 N-C 2/3'' 0.63x (ocular)
  • Brightfield scanner equipped with Merlin Camera F-146C IRF MEDICAL (ALLIED Vision Technologies Medical)
  • Fluorescence scanner equipped with a HXP 120 illuminator (reflected-light source) and an AxioCam MRm Rev. 3 Camera
  • Filtersets presently available at the fluorescence scanner turret (all Carl Zeiss):
  • -           Filterset 02
  • -           Filterset 27
  • -           Filterset 38
  • -           Filterset 43
  • -           Filterset 49
  • -           Filterset 50
  • -           Filterset 63
  • Software: both scanners controlled by MIRAX MIDI control software version 1,12,25,1, developed by 3D HISTECH.
  • List of replaceable filtersets available at the Witter group:
  • -           01
  • -           02
  • -           09
  • -           14
  • -           15
  • -           18
  • -           20
  • -           23
  • -           24
  • -           27
  • -           28
  • -           38
  • -           43
  • -           44
  • -           49
  • -           50
  • -           63
  • -           75 HE
  • -           DIC
  • (Carl Zeiss)
  • -           UV 11000V3
  • -           Cy3 31002
  • (Nikon)

FACILITIES FOR ELECTROPHYSIOLOGICAL RECORDINGS

Technical data

Neuralynx electrophysiological recordings system:

  • 16-40kHz Sample Rates in 2kHz Steps
  • 16 Board Capable (512 Channels)
  • Multiple Gigabit Ethernet Over Fiber Data Connections
  • Automatic Configuration for Board Population
  • Four 8-Bit I/O Ports
  • 4 Front Panel D/A Outputs (4 Outputs Through 2 Stereo Jacks with a +- 1 Volt Input Range)
  • Automatic Firmware Version Updates Loading From The PC
  • Multiple Digital Lynx Synchronization Unlimited Channels
  • High Speed Serial Expansion (For Future External Devices)
  • Mezzanine Board Expansion (For Major Onboard Processing Capabilities) Analog Or Digital I/O)
  • AC, DC and Hybrid AC/DC Coupled Signal Inputs

Axona electrophysiological recordings system:

  • Compact multi-channel data acquisition system designed to work with any PC
  • having a USB 2.0 port.
  • Designed for "tetrode" (twisted quadruple electrode) recording. During
  • recording, spike events trigger data capture on all 4 channels in a group. The
  • triggering can also be configured to record signals on pairs of channels
  • (stereotrodes) or single independent electrodes as required.
  • Amplifier modules support 16 channels each, and 4 modules can be
  • accommodated in a single unit, for a total of 64 channels.
  • Easy software selection and routing of signal and reference channels.
  • Low frequency signals such as EEG can also be recorded on all channels.
  • An integrated or external video tracker can be synchronised with unit
  • recording so that spike events can be correlated with position events. 

Surgery facilities

All of our surgery rooms are equipped with:

  • -Isofluran gas anaesthesia setup
  • -Zeiss Stemi 2000 microscope
  • -Zeiss Light source
  • -High Speed Stereotaxic Drill (220V) 0 – 45000 rpm.
  • -Heatpad
  • -Stereotaxic equipment (all Kopf) :
  • Model 1430-B Stereotaxic Frame (for rats)
  • Model 900 Stereotaxic "U" Frame (for mice)
  • Micro Manipulator (Manipulator X, Z adjustment - Metric vernier scale 80 mm travel. Calibrated dial - 10 micron increments,1.0 mm of travel per revolution.)
  • Electrode Manipulator (Manipulator X, Z adjustment - 80 mm travel calibrated 0.1 mm vernier scale (100 micron increments), 3.0 mm advance per revolution.)
  • UltraMicroPump (UMP3) (one) with SYS-Micro4 Controller (can dispense as little as 600 picoliters)

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Animal facility infrastructure

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