Centre for Molecular Imaging at NT (MINT)

Total internal reflection microscope (TIRF)

– Zeiss Laser TIRF I

Tatyana Sherstova using the high resolution TIRF microscope. Photo: Per Henning/NTNU

Specifications

The microscope

Zeiss Axio Observer.Z1 inverted microscope with control touchscreen.

Focus drive

Motorized objective nosepiece with hardware-based focus stabilizer.

Stages and incubation accessories

  • Mechanical stage from Zeiss
  • The baseplate scan stage from Bruker AXS with standard sample holder.
  • A heater stage for the baseplate, including temperature controller
  • Perfusion accessories from Bruker AXS

Objectives

Magnification NA Type Immersion Coverslip WD (mm)
10x 0.3 Plan Neofluar Ph1 Air 0.17 5.6
20x 0.5 Plan Neofluar Air 0.17 2.0
40x 0.75 Plan-Neofluar Air 0.17 0.71
100x 1.54 Alpha Plan-Fluar Oil 0.17 0.11

In addition, a 1.6 lense is located in the Optovar turret position

Condenser top

NA 0.55, air, working distance 26 mm

Fluorescence filter sets

Name Excitation filter Emission filter Dichroic
Filter Set 51 BP 453 - 465 nm BP 470 - 500 nm 470 nm
Filter Set 52 BP 483 - 493 nm BP 500 - 550 nm 500 nm
Filter Set 53 BP 540 - 552 nm BP 550 - 600 nm 540 nm
Filter Set 74 HE BP 465 - 495 nm BP 510 - 536 nm 505 nm
BP 552 - 577 nm BP 588 - 645 nm 583 nm

Lamps

Halogen: 12V-100W

Mercury arc lamp HXP 120W

Lasers

TIRF multiline Argon-laser 458, 488, 514 nm (25 mW) intensity of the excitation laser light.

TIRF-slider

Manual version I

Camera

Andor iXon DU 897_BV EMCCD from Andor Technologies

Software on the LSM computer

ZEN BLUE 2012 with the modules

  1. ZEN Module Measurements
  2. ZEN Module Multi Channel
  3. ZEN Module Image analysis
  4. ZEN Module Time Lapse
  5. ZEN Module Z stack
  6. ZEN Module Extended Focus
  7. ZEN Module Autofocus
  8. ZEN Module Colocalization

Andor Solis for Imaging, version 4.22, from Andor Technologies

Mon, 31 Aug 2015 14:54:26 +0200

Technical support

Optical/confocal microscopy
Image analysis

Contact: Astrid Bjørkøy


Atomic force microscopy (MultiMode8)

Contact: Gjertrud Maurstad


Flow cytometry

Contact: Kristin Grendstad Sæterbø