Course content

The course gives a theoretical as well as a practical introduction to chromatographic methods and techniques: adsorption and partition chromatography, thin layer chromatography (TLC), liquid column chromatography (including HPLC), gas chromatography (GC), ion exchange, size exclusion and supercritical fluid chromatography (SFC). Coupled chromatographic/spectrometric techniques (GC-MS etc.), sample preparation techniques, (solid phase extraction, SPE) and methods for chiral separations will also be discussed.

Learning outcome

The course aims at giving a basic theoretical and practical understanding of chromatography. The student shall be able to choose and use appropriate chromatographic techniques for solving separation problems. Laboratory exercises shall provide the student with some experience on how to apply, in practice, selected chromatographic techniques on relevant examples.

Learning methods and activities

Lectures (3 hours per week). Laboratory exercises (4 hours per week, all reports must be approved in order to get access to the final examination). Two midterm examinations and two projects (3 of these 4 activities must be approved before the final examination).
For re-sit or extraordinary examinations, written examination may be changed to oral examination.

Compulsory assignments

• Approved laboratory course
• Approved exercises/Midterm examinations

Required previous knowledge

Knowledge corresponding to KJ1000 and KJ1020/TKJ4100 or corresponding courses.

Course materials

T. Greibrokk, J. Karlsen og K.E. Rasmussen: Kromatografi, 3. utg., Universitetsforlaget, Oslo, 1998 (ISBN 82-13-02356-0).

Credit reductions