MEDT8010 - Metabolomics- Methods and Applications
Lessons are not given in the academic year 2014/2015
Metabolism is the set of chemical reactions that occur in living organisms in order to maintain life. Metabolomics is the systematic study of metabolic profiles in biological samples and tissues. It is the newest omics science, and a complementary tool to genomics and proteomics in systems biology.
The course gives an introduction to the use of metabolomics. The objectives are to give participants knowledge of advanced NMR and MS techniques, including hands-on experience with processing and analysis of such data, used in metabolomics.
Theoretical and practical aspects of these methods, including sample preparation, data acquisition, processing and further analysis will be discussed.
The course will also cover NMR and MS based metabolomics applied to various types of samples. Requirements, information content as well as strengths and limitations of metabolomics will be included.
The role of metabolomics in biological and medical research as well as clinical use will be illustrated.
After completing the course, the student:
1 Should understand the principles of metabolomics.
2 Should be able to evaluate and apply the appropriate experimental design in a given metabolomics research question (including sample processing, choiche of methods and analytical strategies).
3 Should be able to apply advanced NMR and MS methods in metabolomics experiments.
4 Should be able to analyse and interpret NMR and MS data.
5 Should be able to critically evaluate metabolomics results and publications.
6 Should be able to write a review paper at international publication level about a chosen topic within metabolomics.
Learning methods and activities
Lectures, teamwork with relevant scientific papers and hands-on exercises. The course will be arranged with a minimum of 4 students.
The course will be held as an intensive course, 6 fully days distributed on two weeks. These weeks will either take place subsequently or be arranged a couple of weeks apart. Following activities will take place:
- lectures (32 hours)
- hands-on exercises (teamwork). Written reports/summaries from the exercises/teamwork will be requested(10 hours)
- laboratory exercises (16 hours)
- self study (130 hours)
- home exam
Participation in lectures, exercises and lab is obligatory, and a prerequisite to be allowed to deliver the home exam.
Exam registration requires that class registration is approved in the same semester, or that compulsory activities are approved in a previous semester.
Recommended previous knowledge
Some basic experience in practical/theoretical high resolution magnetic resonance spectroscopy (NMR/MRS), gas/liquid chromatography (GC/LC) and mass spectrometry (MS).
Required previous knowledge
Master's Degree or similar. Medical students at The Student Research Programme. Candidates with a lower degree will be assessed individually.
Teaching material will be found at It's learning.