Facilities in the Electron Microscopy Laboratory (EMLab)

Cellular and Molecular Imaging (CMIC)

Facilities in the Electron Microscopy Laboratory (EMLab)

Equipment and instruments in the EMLab

Equipment and instruments in the EMLab

Teneo Volume Scope electron microscope, SEM with inbuild ultramicrotome (FEI)

Tecnai 12 Biotwin with EDX (Fei Company). Tecnai 12 is a high contrast TEM.

Tecnai 12 is equipped with additional equipment for X‐ray microanalysis (EDX) and scanning transmission (STEM), use for the elemental analysis or chemical characterization of a sample. Montaging pictures can be acquired using the TIA software.

Modell/type: FEI Company Tecnai 12

  1. Acquired in 2001
  2. Filament: Tungsten
  3. Accelerating voltage: up to 120 kV, operate at 80 kV
  4. Imaging modes: Brightfield, Darkfield, STEM
  5. Resolution: 0,2 nm
  6. Magnifications: from 30x
  7. Image capture: MORADA CCD (3392x2248x16bit)
  8. Ditabis digital imaging plates
  9. Detector: EDX
  10. Software: iTEM and TIA
photo mic 1

TEM/STEM (pdf), Tecnai 12 Biotwin with EDX (Fei Company)

JEOL JEM‐1011 is a high resolution TEM. The transmission electron microscope (TEM) are equipped with digital CCD camera (MORADA). Montaging pictures can be acquired using the iTEM software.

Modell/type: JEOL JEM‐1011

  1. Acquired in 2005
  2. Filament: Tungsten
  3. Accelerating voltages: up to 100 kV, operate at 80 kV
  4. Resolution: 0,2 nm
  5. Magnifications: from 30x 
  6. Image capture: MORADA CCD (3392x2248x16bit)
  7. Ditabis digital imaging plates
  8. Software: iTEM
High vacuum system JEOL JEM‐1011
JEOL JEM‐1011
photo mic 2

TEM/STEM (pdf), Tecnai 12 Biotwin with EDX (Fei Company)

Before most biological specimens can be studied with a TEM, they must be made thin enough (≤ 200nm) to allow an electron beam to pass through them.

The ultramicrotomes can be used for semi‐ and ultrathin sectioning of resin embedded materials as well as smooth surfaces of biological and industrial samples for TEM, SEM and LM examination. Cryo‐ultramicrotomy is performed by the EM FC6 cryochamber. It is designed for low temperature sectioning of samples at temperatures from ‐15 to ‐160°C. The instrument can be used for preparation of semi‐ and ultrathin cryosections as well as smooth surfaces of biological and industrial samples for TEM, SEM and LM examination.

Modell/type: Leica EM UC6

  • Acquired in 2005 (2 units)
  • Semi‐ and ultrathin sectioning (3μm – 50nm)

Modell/type: RMC MT‐X

  • Acquired in 1999 (1 unit)
  • Semi ‐ and ultrathin sectioning (3μm – 150nm)

 

Ultramicrotomes (pdf), EM UC7/UC6 and MT-X (Leica and RMC)

Before most biological specimens can be studied with a TEM, they must be made thin enough (≤ 200nm) to allow an electron beam to pass through them.

Glass knives can be used for trimming and semithin sectioning, and a cryo‐diamond knife for ultrathin sectioning.

Modell/type: Leica KMR2

  • Acquired in 1999 (1 unit)
  • Making glass knife

Modell/type: Leica KMR3

  • Acquired in 2013 (1 unit)
  • Making glass knife

 

Ultramicrotomes (pdf), EM UC7/UC6 and MT-X (Leica and RMC)

Modell/type: Leica EMFC6, cryounit

  • Acquired in 2004 (1 unit)
  • Low temperature sectioning system for preparations of semi‐ and ultrathin cryosections (3μm ‐ 60nm) for TEM and smooth surface for SEM. With a temperature range from ‐15°C to ‐160°C.

Ultramicrotomes (pdf), EM UC7/UC6 and MT-X (Leica and RMC)

The Leica VT1000S fully automated Vibratome uses a vibrating razor blade to cut sections from 1‐999 microns under physiological conditions without freezing or embedding, and thus ultrastructure is well preserved. The tissue can be fixed or fresh when using a vibratome.

The vibratome maintains cell morphology, enzyme activity and cell activity. The microtome is well used for immunocytochemistry and histoenzymology.

Ultimately, vibratome tissue sections can be trimmed down and used for high pressure freezing (HPF).

Modell/type: Leica VT1000S

  • Acquired in 2005 (1 unit)
  • Section thickness: 1‐999μm

 

Vibratome (pdf), VT1000S (Leica)

This device is used to freeze substitute vitrified biological samples.

Freeze substitution is a process in which the water molecules within the samples are exchanged with a solvent (usually acetone or methanol), and then replaced with a resin (Epoxy, LR White or Lowicryl). Polymerization of the resin is performed outside when using Epoxy resin or inside the machine when using Lowicryl. This latter resin is polymerized under a UV lamp, starting at ‐45°C, then gradually moving up to room temperature (case with Lowicryl HM20).

At the end of the process hard plastic blocks are generated ready to be cut by an ultramicrotome.  

Modell/type: Leica EM AFS

  • Acquired in 2004 (1 unit)
  • Embedding system: Capsules and flat block
  • Equipped with LED UV lamp unit for polymerization
  • With a temperature range from +70°C to ‐140°C

Freeze substitution unit (pdf), EM AFS (Leica)

Critical point dryer (Polaron)

Bulk biological specimen (along with hydrated materials) must be dried rapidly, without introducing artefacts, before they can be imaged in a SEM. The critical point dryer is the most efficient instrument for quickly drying specimens without the shrinking or cracking that accompanies other methods. Critical point dryer (pdf) (Polaron)

  • Modell/type: Polaron
  • Acquired in 1978 (1 unit)

 

Critical point dryer, CPD300 (Leica)

 

 

Modell/type: Polaron

  • Acquired in 1982 (1 unit)
  • The sputter coater is used to apply a conductive metal film (nm) on non‐conductive SEM specimens.
  • Coating material: Gold/Palladium (Au/Pd)

 

Sputter coater (pdf) (Polaron)

Film scanner for digitalization (Nikon Coolscan)

Sputtercoater, EM ACE600 (Leica)

PC workstation with Amira softeare for 3D reconstruction

Software: Radius, iTEM, TIA, Amira, MAPS

Contact

Contact

​​​​​​​ CMIC Electron Microscopy lab

Address

CMIC EMLab, Department of Clinical and Molecular Medicine, NO-7491 Trondheim, Norway

Location

Erling Skjalgsons gate 1, Laboratoriesenteret 4. floor, LS 232.04.035

Corona virus

Corona virus

Cellular & Molecular Imaging Core facility (CMIC) has limited capacity due to the corona situation  If you have any questions please use the contact us-link or contact Bjørnar Sporsheim.
30 Apr 2021

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