KJ3059 - Chromatography, Advanced Course


Examination arrangement

Examination arrangement: Written examination
Grade: Letters

Evaluation form Weighting Duration Examination aids Grade deviation
Written examination 100/100 4 hours C

Course content

Techniques of, mostly chromatography-related, modern analytical separation and quantification methods are presented. Topics presented are a.o. chromatographic theory including the solvation parameter model of retention; some advanced aspects of liquid chromatography (HPLC) and gas chromatography (GC); 2-dimensional chromatography (coupled methods chromatography-chromatography); 'tandem techniques' based on on-line coupling of separation methods and spectrometry; mass spectrometry, with focus on applications in connection with analytical separation methods;
Other relevant topics can be included, such as capillary electromigration separation methods, supercritical fluid chromatography, stereoisomer separation and preparative chromatography techniques. Students choose one of them for self-studying, as an elective part of the final examination.
Laboratory exercises apply some of these concepts, a.o. an on-line coupled method. Exercises provide the possibility for some repetition of basic concepts, discussions of relevant applications from current research papers and presentation of special topics.
This course has nearly the same content, and is taught together with, KJ8059.

Learning outcome

At the end of the course a student should be able to:

- explain the principles of a wide range of modern analytical methods of separation and quantitation;
- evaluate strengths and limitations of different separation and detection techniques with respect to sample properties and to specific analytical problems;
- choose and plan the use of suitable analytical separation techniques for actual analytical problems based on a sound knowledge of the potentially relevant methods;
- judge the quality of his/her own, or others', analytical results from analytical separation methods, and to evaluate the chosen operational procedures;
- understand and if necessary adapt to own requirements, published procedures, e.g. to locally available technical resources or to more modern practices;
- apply analytical methods of separation and quantitation in an effective and reasonable way after only a minimum of practical teaching;
- consider HSE in relation to chromatographic experimentation.

Learning methods and activities

Lectures (3 hours per week), exercises (1 hour per week), and laboratory exercises (40 hours). All laboratory exercise reports must be approved in order to get access to the final examination. HSE instruction will be given to students that have not received such chromatography-specific information earlier (e.g. in KJ2053). This instruction is a prerequisite to get access to the lab. course. It is given before start-up of the laboratory activities, typically in October (time to be agreed on).
English teaching by request. When lectures and exercises are given in English, the examination text can be given in English only. For re-sit exam or exam during a term without lectures in this course, written examination may be changed to oral examination.

Compulsory assignments

  • Laboratory execises

Further on evaluation

In case of a re-sit examination only the written exam is repeated.

For the Academic year 2017/2018 §28 of the Supplementary regulations of the Study regulations, dated 2015.08.01, apply for re-sit examinations for final oral and written examinations in science subjects.

Specific conditions

Exam registration requires that class registration is approved in the same semester. Compulsory activities from previous semester may be approved by the department.

Course materials

To be announced by us at the beginning of the course. (Possibly: C. F. Poole, The essence of chromatography, 1.edn., 2003, Elsevier, but also additional and/or alternative reading matter).

Credit reductions

Course code Reduction From To
KJ3058 7.5 2012-09-01
KJ8058 7.5 2012-09-01
KJ8059 7.5 2012-09-01


Detailed timetable


Examination arrangement: Written examination

Term Statuskode Evaluation form Weighting Examination aids Date Time Room *
Autumn ORD Written examination 100/100 C 2017-12-13 09:00 KJL21 , E4
Spring ORD Written examination 100/100 C
  • * The location (room) for a written examination is published 3 days before examination date.
If more than one room is listed, you will find your room at Studentweb.