Background and activities
Stokke leads a biophysics based research activity at the interface between life-science and engineering, with emphasis on physical properties of biological macromolecules and their assemblies. Mesoscale structure formation and interactions within biopolymers are fundamental phenomena underpinning molecular understanding of biopolymers and their technological exploitation. We study details of biopolymer interactions, association states occurring within polyelectrolyte complexes, biopolymer assemblies and multilayers, polysaccharide gels, and responsive gels as biospecific signal transducers. The activity covers a range of various biological macromolecules and their properties domains as exemplified by information provided in the following links:
Single-molecular pair interactions
Bioresponsive hydrogels as signal transducing materials in biosensors
Structure and properties of beta-glucans
Microfluidics – devices for preparation of hydrogels with controlled size, and separation of exosomes
Amyloid structures studied by AFM and TIRFM
Structure of polysaccharide hydrogels
Nanoscopic characterization of Toll-like receptors
Physics of the enzymatic mode of action
In addition to classical ensemble averaging techniques, application of single-molecule techniques is a distinctive facet of our approach to tackle core issues within these topics. The tools implemented locally for this research include atomic force microscopy, total internal reflection fluorescence microscopy, dynamic force spectroscopy, high resolution interferometry and rheology. These tools are complemented by additional techniques made available to through collaborations with laboratories either locally, or within national and international collaborations. We are also increasing taking advantage of parts of the micro-and nanoscale fabrication facilities provided by NTNU NanoLab, e.g., for custom design of fabricated microfluidic chips. A distintiive feature of the research efforts is also the combination of experimental approaches with numerical/theoretical ones, e.g, finite element modelling of bioresponsive hydrogels, modelling of enzymatic mode of action, and implementation of efficient routines for extraction of quantitative parameters (e.g. force spectroscopy, image analysis).
The list of publications are avaible at:
Scientific, academic and artistic work
A selection of recent journal publications, artistic productions, books, including book and report excerpts. See all publications in the database
- (2015) Direct Determination of Chitosan–Mucin Interactions Using a Single-Molecule Strategy: Comparison to Alginate–Mucin Interactions. Polymers. vol. 7 (2).
- (2015) Single molecule study of heterotypic interactions between mucins possessing the Tn cancer antigen. Glycobiology. vol. 25 (5).
- (2015) Energy Landscape of Alginate-Epimerase Interactions Assessed by Optical Tweezers and Atomic Force Microscopy. PLoS ONE.
- (2015) CD14, TLR4 and TRAM Show Different Trafficking Dynamics During LPS Stimulation. Traffic : the International Journal of Intracellular Transport. vol. 16 (7).
- (2015) Novel imaging technologies for characterization of microbial extracellular polysaccharides. Frontiers in Microbiology. vol. 6 (MAY).
- (2014) Polyelectrolyte and antipolyelectrolyte effects in swelling of polyampholyte and polyzwitterionic charge balanced and charge offset hydrogels. European Polymer Journal. vol. 53 (1).
- (2013) The relation of apple texture with cell wall nanostructure studied using an atomic force microscope. Carbohydrate Polymers. vol. 92 (1).
- (2013) High resolution interferometry as a tool for characterization of swelling of weakly charged hydrogels subjected to amphiphile and cyclodextrin exposure. Journal of Colloid and Interface Science. vol. 390 (1).
- (2013) Cyclodextrin triggered dimensional changes of polysaccharide nanogel integrated hydrogels at nanometer resolution. Soft Matter. vol. 9 (21).
- (2013) PEGylated chitosan complexes DNA while improving polyplex colloidal stability and gene transfection efficiency. Carbohydrate Polymers. vol. 94 (1).
- (2013) Evidence for Age-Dependent in Vivo Conformational Rearrangement within Aβ Amyloid Deposits. ACS Chemical Biology. vol. 8 (6).
- (2013) Swelling of a hemi-ellipsoidal ionic hydrogel for determination of material properties of deposited thin polymer films: an inverse finite element approach. Soft Matter. vol. 9.
- (2013) Higher order structures of a bioactive, water-soluble (1→3)-b-d-glucan derived from Saccharomyces cerevisiae. Carbohydrate Polymers. vol. 92 (2).
- (2013) Structure-Function Relationships in Glycopolymers: Effects of Residue Sequences, Duplex, and Triplex Organization. Biopolymers. vol. 99 (10).
- (2012) Impregnation of weakly charged anionic microhydrogels with cationic polyelectrolytes and their swelling properties monitored by a high resolution interferometric technique. Transformation from a polyelectrolyte to polyampholyte hydrogel. European Polymer Journal. vol. 48 (11).
- (2012) Enhanced Self-Association of Mucins Possessing the T and Tn Carbohydrate Cancer Antigens at the Single-Molecule Level. Biomacromolecules. vol. 13 (5).
- (2012) Direct measurement of the interaction force between immunostimulatory CpG-DNA and TLR9 fusion protein. Journal of Molecular Recognition. vol. 25 (2).
- (2012) Isothermal Titration Calorimetry Study of the Polyelectrolyte Complexation of Xanthan and Chitosan Samples of Different Degree of Polymerization. Biopolymers. vol. 97 (1).
- (2012) Nanoscopic and Photonic Ultrastructural Characterization of Two Distinct Insulin Amyloid States. International Journal of Molecular Sciences. vol. 13 (2).
- (2012) Oligoguluronate induced competitive displacement of mucin-alginate interactions: relevance for mucolytic function. Soft Matter. vol. 8 (32).