Maria Karoline Andersen

About

I am a postdoctoral fellow at the MR Cancer group, Department of Circulation and Medical Imaging (ISB), Faculty of Medicine (DMF).

Research focus

My research of interest is metabolic profiling of prostate cancer tissue. For this purpose, I use methods which include high-resolution magic angle spinning (HR-MAS) magnetic resonance spectroscopy (MRS), matrix assisted laser desorption/ionization (MALDI) mass spectrometry imaging, immunohistochemistry (IHC), various mRNA analysis methods and multivariate analysis. A main aim of my research is to identify metabolic and transcriptomic markers/signatures that can illuminate the cell biology of prostate cancer tissue.

Education

2013-2015: Master's degree in Molecular Medicine, DMF, NTNU
2010-2013: Biotechnology, Faculty of Natural Sciences and Technology, NTNU

Previous academic work and projects

Master's thesis: MicroRNA profiling using formalin-fixed paraffin-embedded (FFPE) tissue - Quality assessment of microRNA profiles from FFPE and matched fresh frozen prostate tissue in relation to storage time by applying Next Generation Sequencing.
iGEM 2013 competition participation. Project: VesiColi.

Publications

Spatial differentiation of metabolism in prostate cancer tissue by MALDI-TOF MSI

Ved bruk av massespektrometrisk avbildning kunne vi finne den romlige lokaliseringen metabolitter og lipider i prostatakreftvev.

Simultaneous Detection of Zinc and Its Pathway Metabolites Using MALDI MS Imaging of Prostate Tissue

Sink er en foreslått biomarkør for prostatakreft ettersom flere studier har rapportert reduserte mengder ved kreftutvikling. Vi kunne for første gang avbilde sink samtidig som relevate metabolitter (sitrat og aspertat) i prostatakreftvev.

2025

Krossa, Sebastian; Andersen, Maria K.; Midtbust, Elise; Wess, Maximilian; Antti, Kiviaho,; Sharma, Abhibhav. (2025) Spatial multi-omics identifies aggressive prostate cancer signatures highlighting pro-inflammatory chemokine activity in the tumor microenvironment | Nature Communications. Nature Communications
Academic article
Andersen, Maria K.; Tessem, May-Britt; Abrahamsen, Bendik Skarre; Telumyan, Elen; Guillem, Juan Carlos Cabellos. (2025) Tumorteltet på Researchers' Night 2025.
Other presentation
Wess, Maximilian; Andersen, Maria Karoline; Midtbust, Elise; Guillem, Juan Carlos Cabellos; Viset, Trond; Størkersen, Øystein. (2025) Spatial integration of multi-omics data from serial sections using the novel Multi-Omics Imaging Integration Toolset. GigaScience
Academic article
Pedersen, Christine Aaserød; Fleischer, Thomas; Wess, Maximilian; Midtbust, Elise; Andersen, Maria Karoline; Viset, Trond. (2025) DNA methylation in normal-appearing tissue associated with prostate cancer recurrence and metastasis. Clinical Epigenetics
Academic article

2024

Wess, Maximilian; Andersen, Maria Karoline; Midtbust, Elise; Guillem, Juan Carlos Cabellos; Viset, Trond; Størkersen, Øystein. (2024) Spatial Integration of Multi-Omics Data using the novel Multi-Omics Imaging Integration Toolset. BioRxiv
Academic article
Andersen, Maria K.; Krossa, Sebastian; Midtbust, Elise; Pedersen, Christine Aaserød; Wess, Maximilian; Høiem, Therese Stork. (2024) Spatial transcriptomics reveals strong association between SFRP4 and extracellular matrix remodeling in prostate cancer. Communications Biology
Academic article
Kiviaho, Antti; Eerola, Sini K.; Kallio, Heini M.L.; Andersen, Maria K.; Hoikka, Miina; Salonen, Iida. (2024) Single cell and spatial transcriptomics highlight the interaction of club-like cells with immunosuppressive myeloid cells in prostate cancer. Nature Communications
Academic article

2023

Andersen, Maria Karoline; Krossa, Sebastian; Midtbust, Elise; Pedersen, Christine Aaserød; Wess, Maximilian; Høiem, Therese Stork. (2023) Spatial transcriptomics reveals strong association between SFRP4 and extracellular matrix remodeling in prostate cancer. Research Square
Academic article
Andersen, Maria Karoline; Giampà , Marco; Midtbust, Elise; Høiem, Therese Stork; Krossa, Sebastian; Tessem, May-Britt. (2023) Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging. Methods in molecular biology
Academic article
Giampà , Marco; Andersen, Maria Karoline; Krossa, Sebastian; Denti, Vanna; Smith, Andrew; Moestue, Siver Andreas. (2023) Visualization of Small Intact Proteins in Breast Cancer FFPE Tissue. Methods in molecular biology
Academic article

2022

Buchholz, Rebecca; Krossa, Sebastian; Andersen, Maria Karoline; Holtkamp, Michael; Sperling, Michael; Karst, Uwe. (2022) A simple preparation protocol for shipping and storage of tissue sections for laser ablation-inductively coupled plasma-mass spectrometry imaging. Metallomics
Academic article
Krossa, Sebastian; Andersen, Maria Karoline; Midtbust, Elise; Viset, Trond; Størkersen, Øystein; Giskeødegård, Guro. (2022) Inflammatory chemokines upregulated in the tumor microenvironment of prostate cancer patients experiencing relapse after radical prostatectomy. European Urology
Abstract
Høiem, Therese Stork; Andersen, Maria Karoline; Martin-Lorenzo, Marta; Longuespée, Rémi; Claes, Britt S.R.; Nordborg, Anna. (2022) An optimized MALDI MSI protocol for spatial detection of tryptic peptides in fresh frozen prostate tissue. Proteomics
Academic article
Andersen, Maria Karoline; Krossa, Sebastian; Midtbust, Elise; Viset, Trond; Størkersen, Øystein; Giskeødegård, Guro F.. (2022) Multiomics spatial mapping of lipid metabolism in prostate cancer. European Urology
Abstract

2021

Andersen, Maria Karoline; Høiem, Therese Stork; Claes, Britt S.R.; Balluff, Benjamin; Marta, Martin-Lorenzo; Richardsen, Elin. (2021) Spatial differentiation of metabolism in prostate cancer tissue by MALDI-TOF MSI. Cancer & Metabolism
Academic article
Denti, Vanna; Andersen, Maria Karoline; Smith, Andrew; Bofin, Anna M.; Nordborg, Anna; Magni, Fulvio. (2021) Reproducible lipid alterations in patient-derived breast cancer xenograft FFPE tissue identified with MALDI MSI for pre-clinical and clinical application. Metabolites
Academic article

2020

Andersen, Maria Karoline; Krossa, Sebastian; Høiem, Therese Stork; Buchholz, Rebecca; Claes, Britt S.R.; Balluff, Benjamin. (2020) Simultaneous detection of zinc and its pathway metabolites using MALDI MS imaging of prostate tissue. Analytical Chemistry
Academic article
Andersen, Maria Karoline; Giskeødegård, Guro F.; Tessem, May-Britt. (2020) Metabolic alterations in tissues and biofluids of patients with prostate cancer. Current Opinion in Endocrine and Metabolic Research
Academic literature review

2019

Andersen, Maria Karoline; Krossa, Sebastian; Høiem, Therese Stork; Buchholz, Rebecca; Claes, Britt S.R.; Balluff, Benjamin. (2019) Simultaneous detection of the cancer biomarkers zinc and citrate in prostate cancer tissue using mass spectrometry imaging. European Urology. Supplement
Abstract
Andersen, Maria Karoline; Tessem, May-Britt; Bathen, Tone F.; Giskeødegård, Guro F.; Rye, Morten B.. (2019) Multi-omics molecular profiling of prostate cancer. Norges teknisk-naturvitenskapelige universitet Norges teknisk-naturvitenskapelige universitet
Doctoral dissertation

2018

Andersen, Maria Karoline; Rise, Kjersti; Giskeødegård, Guro F.; Richardsen, Elin; Bertilsson, Helena; Størkersen, Øystein. (2018) Integrative metabolic and transcriptomic profiling of prostate cancer tissue containing reactive stroma. Scientific Reports
Academic article
Andersen, Maria Karoline; Sandsmark, Elise; Tessem, May-Britt. (2018) Økt genuttrykk av SFRP4 i aggressiv prostatakreft. Bestpractice Onkologi/Hematologi
Article in business/trade/industry journal
Euceda, Leslie R.; Andersen, Maria Karoline; Tessem, May-Britt; Moestue, Siver Andreas; Grinde, Maria Tunset; Bathen, Tone Frost. (2018) NMR-Based Prostate Cancer Metabolomics. Methods in molecular biology
Academic article
Rye, Morten Beck; Bertilsson, Helena; Andersen, Maria Karoline; Rise, Kjersti; Bathen, Tone Frost; Drabløs, Finn. (2018) Cholesterol synthesis pathway genes in prostate cancer are transcriptionally downregulated when tissue confounding is minimized. BMC Cancer
Academic article

2017

Sandsmark, Elise; Andersen, Maria Karoline; Bofin, Anna M.; Bertilsson, Helena; Drabløs, Finn; Bathen, Tone Frost. (2017) SFRP4 gene expression is increased in aggressive prostate cancer. Scientific Reports
Academic article

Journal publications

Krossa, Sebastian; Andersen, Maria K.; Midtbust, Elise; Wess, Maximilian; Antti, Kiviaho,; Sharma, Abhibhav. (2025) Spatial multi-omics identifies aggressive prostate cancer signatures highlighting pro-inflammatory chemokine activity in the tumor microenvironment | Nature Communications. Nature Communications
Academic article
Wess, Maximilian; Andersen, Maria Karoline; Midtbust, Elise; Guillem, Juan Carlos Cabellos; Viset, Trond; Størkersen, Øystein. (2025) Spatial integration of multi-omics data from serial sections using the novel Multi-Omics Imaging Integration Toolset. GigaScience
Academic article
Pedersen, Christine Aaserød; Fleischer, Thomas; Wess, Maximilian; Midtbust, Elise; Andersen, Maria Karoline; Viset, Trond. (2025) DNA methylation in normal-appearing tissue associated with prostate cancer recurrence and metastasis. Clinical Epigenetics
Academic article
Wess, Maximilian; Andersen, Maria Karoline; Midtbust, Elise; Guillem, Juan Carlos Cabellos; Viset, Trond; Størkersen, Øystein. (2024) Spatial Integration of Multi-Omics Data using the novel Multi-Omics Imaging Integration Toolset. BioRxiv
Academic article
Andersen, Maria K.; Krossa, Sebastian; Midtbust, Elise; Pedersen, Christine Aaserød; Wess, Maximilian; Høiem, Therese Stork. (2024) Spatial transcriptomics reveals strong association between SFRP4 and extracellular matrix remodeling in prostate cancer. Communications Biology
Academic article
Kiviaho, Antti; Eerola, Sini K.; Kallio, Heini M.L.; Andersen, Maria K.; Hoikka, Miina; Salonen, Iida. (2024) Single cell and spatial transcriptomics highlight the interaction of club-like cells with immunosuppressive myeloid cells in prostate cancer. Nature Communications
Academic article
Andersen, Maria Karoline; Krossa, Sebastian; Midtbust, Elise; Pedersen, Christine Aaserød; Wess, Maximilian; Høiem, Therese Stork. (2023) Spatial transcriptomics reveals strong association between SFRP4 and extracellular matrix remodeling in prostate cancer. Research Square
Academic article
Andersen, Maria Karoline; Giampà , Marco; Midtbust, Elise; Høiem, Therese Stork; Krossa, Sebastian; Tessem, May-Britt. (2023) Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging. Methods in molecular biology
Academic article
Giampà , Marco; Andersen, Maria Karoline; Krossa, Sebastian; Denti, Vanna; Smith, Andrew; Moestue, Siver Andreas. (2023) Visualization of Small Intact Proteins in Breast Cancer FFPE Tissue. Methods in molecular biology
Academic article
Buchholz, Rebecca; Krossa, Sebastian; Andersen, Maria Karoline; Holtkamp, Michael; Sperling, Michael; Karst, Uwe. (2022) A simple preparation protocol for shipping and storage of tissue sections for laser ablation-inductively coupled plasma-mass spectrometry imaging. Metallomics
Academic article
Krossa, Sebastian; Andersen, Maria Karoline; Midtbust, Elise; Viset, Trond; Størkersen, Øystein; Giskeødegård, Guro. (2022) Inflammatory chemokines upregulated in the tumor microenvironment of prostate cancer patients experiencing relapse after radical prostatectomy. European Urology
Abstract
Høiem, Therese Stork; Andersen, Maria Karoline; Martin-Lorenzo, Marta; Longuespée, Rémi; Claes, Britt S.R.; Nordborg, Anna. (2022) An optimized MALDI MSI protocol for spatial detection of tryptic peptides in fresh frozen prostate tissue. Proteomics
Academic article
Andersen, Maria Karoline; Krossa, Sebastian; Midtbust, Elise; Viset, Trond; Størkersen, Øystein; Giskeødegård, Guro F.. (2022) Multiomics spatial mapping of lipid metabolism in prostate cancer. European Urology
Abstract
Andersen, Maria Karoline; Høiem, Therese Stork; Claes, Britt S.R.; Balluff, Benjamin; Marta, Martin-Lorenzo; Richardsen, Elin. (2021) Spatial differentiation of metabolism in prostate cancer tissue by MALDI-TOF MSI. Cancer & Metabolism
Academic article
Denti, Vanna; Andersen, Maria Karoline; Smith, Andrew; Bofin, Anna M.; Nordborg, Anna; Magni, Fulvio. (2021) Reproducible lipid alterations in patient-derived breast cancer xenograft FFPE tissue identified with MALDI MSI for pre-clinical and clinical application. Metabolites
Academic article
Andersen, Maria Karoline; Krossa, Sebastian; Høiem, Therese Stork; Buchholz, Rebecca; Claes, Britt S.R.; Balluff, Benjamin. (2020) Simultaneous detection of zinc and its pathway metabolites using MALDI MS imaging of prostate tissue. Analytical Chemistry
Academic article
Andersen, Maria Karoline; Giskeødegård, Guro F.; Tessem, May-Britt. (2020) Metabolic alterations in tissues and biofluids of patients with prostate cancer. Current Opinion in Endocrine and Metabolic Research
Academic literature review
Andersen, Maria Karoline; Krossa, Sebastian; Høiem, Therese Stork; Buchholz, Rebecca; Claes, Britt S.R.; Balluff, Benjamin. (2019) Simultaneous detection of the cancer biomarkers zinc and citrate in prostate cancer tissue using mass spectrometry imaging. European Urology. Supplement
Abstract
Andersen, Maria Karoline; Rise, Kjersti; Giskeødegård, Guro F.; Richardsen, Elin; Bertilsson, Helena; Størkersen, Øystein. (2018) Integrative metabolic and transcriptomic profiling of prostate cancer tissue containing reactive stroma. Scientific Reports
Academic article
Andersen, Maria Karoline; Sandsmark, Elise; Tessem, May-Britt. (2018) Økt genuttrykk av SFRP4 i aggressiv prostatakreft. Bestpractice Onkologi/Hematologi
Article in business/trade/industry journal
Euceda, Leslie R.; Andersen, Maria Karoline; Tessem, May-Britt; Moestue, Siver Andreas; Grinde, Maria Tunset; Bathen, Tone Frost. (2018) NMR-Based Prostate Cancer Metabolomics. Methods in molecular biology
Academic article
Rye, Morten Beck; Bertilsson, Helena; Andersen, Maria Karoline; Rise, Kjersti; Bathen, Tone Frost; Drabløs, Finn. (2018) Cholesterol synthesis pathway genes in prostate cancer are transcriptionally downregulated when tissue confounding is minimized. BMC Cancer
Academic article
Sandsmark, Elise; Andersen, Maria Karoline; Bofin, Anna M.; Bertilsson, Helena; Drabløs, Finn; Bathen, Tone Frost. (2017) SFRP4 gene expression is increased in aggressive prostate cancer. Scientific Reports
Academic article

Report

Andersen, Maria Karoline; Tessem, May-Britt; Bathen, Tone F.; Giskeødegård, Guro F.; Rye, Morten B.. (2019) Multi-omics molecular profiling of prostate cancer. Norges teknisk-naturvitenskapelige universitet Norges teknisk-naturvitenskapelige universitet
Doctoral dissertation

UTSTILLING

Andersen, Maria K.; Tessem, May-Britt; Abrahamsen, Bendik Skarre; Telumyan, Elen; Guillem, Juan Carlos Cabellos. (2025) Tumorteltet på Researchers' Night 2025.
Other presentation

Outreach