Marianne Sandvold Beckwith
About
I hold a Master of Science from NTNU, and have had a particular interest in microscopy methods for biological applications ever since my student days.
I completed my PhD on tuberculosis infection at the Centre for Molecular Inflammation Research (CEMIR), where we used advanced microscopy techniques to discover how tuberculosis bacteria can kill their host cells by creating holes in the plasma membrane, which also triggers an inflammatory response. The microscopy methods we developed proved useful in my subsequent work as a postdoctoral researcher at the European Molecular Biology Laboratory (EMBL) in Heidelberg, where I studied how immune cells migrate so rapidly throughout the body. We discovered that curvature-sensitive proteins in the cells help them navigate around obstacles.
Through my work as a PhD student and postdoctoral researcher, I have gained insight into a wide range of projects across various disciplines—from molecular biology and biophysics to microscopy and method development. I have seen how enabling microscopy methods can be, and I am passionate about sharing this knowledge for the benefit of our research environments at NTNU.
Competencies
Publications
2020
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Beckwith, Kai Sandvold;
Beckwith, Marianne Sandvold;
Ullmann, Sindre;
Sætra, Ragnhild Sofie Ragnhildstveit;
Kim, Hae Lin;
Marstad, Anne.
(2020)
Plasma membrane damage causes NLRP3 activation and pyroptosis during Mycobacterium tuberculosis infection.
Nature Communications
Academic article
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Meås, Hany Zakaria;
Haug, Markus;
Beckwith, Marianne Sandvold;
Louet, Claire;
Ryan, Liv;
Hu, Zhenyi.
(2020)
Sensing of HIV-1 by TLR8 activates human T cells and reverses latency.
Nature Communications
Academic article
2019
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Haug, Markus;
Meås, Hany Zakaria;
Beckwith, Marianne Sandvold;
Louet, Claire;
Ryan, Liv;
Hu, Z..
(2019)
P1511 - Sensing of HIV-1 nucleic acids by TLR8 in human CD4+ T cells.
European Journal of Immunology
Abstract
2018
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Beckwith, Marianne;
Flo, Trude Helen;
Halaas, Øyvind.
(2018)
Targeted correlative light and electron microscopy in the study of host-pathogen interactions.
Norges teknisk-naturvitenskapelige universitet
Norges teknisk-naturvitenskapelige universitet
Doctoral dissertation
2015
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Beckwith, Marianne;
Beckwith, Kai Sandvold;
Sikorski, Pawel;
Skogaker, Nan Elisabeth Tostrup;
Flo, Trude Helen;
Halaas, Øyvind.
(2015)
Seeing a mycobacterium-infected cell in nanoscale 3D: Correlative imaging by light microscopy and FIB/SEM tomography.
PLOS ONE
Academic article
2014
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Østgård, Olderøy Magnus;
Lilledahl, Magnus Borstad;
Beckwith, Marianne;
Melvik, Jan Egil;
Reinholt, Finn P.;
Sikorski, Pawel.
(2014)
Biochemical and Structural Characterization of Neocartilage Formed by Mesenchymal Stem Cells in Alginate Hydrogels.
PLOS ONE
Academic article
2013
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Beckwith, Marianne;
Sikorski, Pawel.
(2013)
Technical Aspects of Ion Milling and Electron Imaging of Epoxy Embedded Samples for FIB/SEM Tomography.
Norges teknisk-naturvitenskapelige universitet
Norges teknisk-naturvitenskapelige universitet
Masters thesis
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Olderøy, Magnus Østgård;
Beckwith, Marianne;
Rrinholt, F P;
Lilledahl, Magnus Borstad;
Sikorski, Pawel;
Brinchmann, Jan E..
(2013)
Identification of functional structures in tissue engineered cartilage: from nm to macro scale.
European Cells and Materials
Abstract
Journal publications
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Beckwith, Kai Sandvold;
Beckwith, Marianne Sandvold;
Ullmann, Sindre;
Sætra, Ragnhild Sofie Ragnhildstveit;
Kim, Hae Lin;
Marstad, Anne.
(2020)
Plasma membrane damage causes NLRP3 activation and pyroptosis during Mycobacterium tuberculosis infection.
Nature Communications
Academic article
-
Meås, Hany Zakaria;
Haug, Markus;
Beckwith, Marianne Sandvold;
Louet, Claire;
Ryan, Liv;
Hu, Zhenyi.
(2020)
Sensing of HIV-1 by TLR8 activates human T cells and reverses latency.
Nature Communications
Academic article
-
Haug, Markus;
Meås, Hany Zakaria;
Beckwith, Marianne Sandvold;
Louet, Claire;
Ryan, Liv;
Hu, Z..
(2019)
P1511 - Sensing of HIV-1 nucleic acids by TLR8 in human CD4+ T cells.
European Journal of Immunology
Abstract
-
Beckwith, Marianne;
Beckwith, Kai Sandvold;
Sikorski, Pawel;
Skogaker, Nan Elisabeth Tostrup;
Flo, Trude Helen;
Halaas, Øyvind.
(2015)
Seeing a mycobacterium-infected cell in nanoscale 3D: Correlative imaging by light microscopy and FIB/SEM tomography.
PLOS ONE
Academic article
-
Østgård, Olderøy Magnus;
Lilledahl, Magnus Borstad;
Beckwith, Marianne;
Melvik, Jan Egil;
Reinholt, Finn P.;
Sikorski, Pawel.
(2014)
Biochemical and Structural Characterization of Neocartilage Formed by Mesenchymal Stem Cells in Alginate Hydrogels.
PLOS ONE
Academic article
-
Olderøy, Magnus Østgård;
Beckwith, Marianne;
Rrinholt, F P;
Lilledahl, Magnus Borstad;
Sikorski, Pawel;
Brinchmann, Jan E..
(2013)
Identification of functional structures in tissue engineered cartilage: from nm to macro scale.
European Cells and Materials
Abstract
Report
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Beckwith, Marianne;
Flo, Trude Helen;
Halaas, Øyvind.
(2018)
Targeted correlative light and electron microscopy in the study of host-pathogen interactions.
Norges teknisk-naturvitenskapelige universitet
Norges teknisk-naturvitenskapelige universitet
Doctoral dissertation
-
Beckwith, Marianne;
Sikorski, Pawel.
(2013)
Technical Aspects of Ion Milling and Electron Imaging of Epoxy Embedded Samples for FIB/SEM Tomography.
Norges teknisk-naturvitenskapelige universitet
Norges teknisk-naturvitenskapelige universitet
Masters thesis
Outreach
2022
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Sætra, Ragnhild Sofie Ragnhildstveit; Ullmann, Sindre; Bugge, Marit. (2022) Host-cell killing by Mycobacterium tuberculosis. Invited talk , Connecticut 2022-04-26 -
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Sætra, Ragnhild Sofie Ragnhildstveit; Ullmann, Sindre; Bugge, Marit. (2022) The inner life of a mycobacterium-infected macrophage. Invited talk , Connecticut 2022-04-27 -
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Sætra, Ragnhild Sofie Ragnhildstveit; Ullmann, Sindre; Bugge, Marit. (2022) The Inner Life of Mycobacterium Infected Macrophages. Gordon Research Conference on Lysosomes and Endocytosis 2022: Physiological Adaptations of the Endo-Lysosomal System , Andover, New Hampshire 2022-06-19 - 2022-06-24
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Bugge, Marit; Sætra, Ragnhild Sofie Ragnhildstveit; Ullmann, Sindre. (2022) The inner life of a mycobacterium-infected macrophage. NIAID Invited talk , Bethesda 2022-05-19 -
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Gidon, Alexandre; Mediaas, Sindre Dahl; Sætra, Ragnhild Sofie Ragnhildstveit. (2022) Spatiotemporal Relations of Mycobacteria and Human Macrophages. Department seminar series , Massachusetts 2022-01-26 -
2021
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Gidon, Alexandre; Ullmann, Sindre; Sætra, Ragnhild Sofie Ragnhildstveit. (2021) Mycobacterium tuberculosis infection in human macrophages. Department seminar series 2021-09-15 -
2020
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PosterBeckwith, Marianne Sandvold; Beckwith, Kai Sandvold; Ullmann, Sindre; Sætra, Ragnhild Sofie Ragnhildstveit; Marstad, Anne; Kim, Haelin. (2020) Plasma membrane damage causes NLRP3 activation and pyroptosis during Mycobacterium tuberculosis infection. Keystone Symposium Tuberculosis , Santa Fe, New Mexico 2020-01-16 - 2020-01-21
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PosterSætra, Ragnhild Sofie Ragnhildstveit; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Ullmann, Sindre; Kim, Haelin; Marstad, Anne. (2020) Mechanisms in human macrophages infected with Mycobacterium tuberculosis. IBA Annual meeting , Zoom 2020-10-26 - 2020-10-27
2019
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Academic lectureHaug, Markus; Meås, Hany Zakaria; Beckwith, Marianne Sandvold; Louet, Claire; Ryan, Liv; Hu, Z.. (2019) Sensing of HIV-1 by TLR8 in human CD4+ T cells. 45th Annual Meeting of the Scandinavian Society for Immunology (SSI) , Geilo 2019-04-02 - 2019-04-05
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Ullmann, Sindre; Sætra, Ragnhild Sofie Ragnhildstveit; Strand, Trine Aakvik. (2019) Balancing life and death in mycobacterium-infected macrophages. 2nd International conference on molecular mechanisms of inflammation , Trondheim 2019-06-03 - 2019-06-06
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Ullmann, Sindre; Sætra, Ragnhild Sofie Ragnhildstveit; Marstad, Anne. (2019) Single-cell dynamics of NLRP3 inflammasome activation and pyroptosis during Mycobacterium tuberculosis infection of human macrophages. IUIS2019 17th International Congress of Immunology , Beijing 2019-10-19 - 2019-10-23
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PosterUllmann, Sindre; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Åsberg, Signe; Strand, Trine Aakvik; Flo, Trude Helen. (2019) Single-cell Dynamics of Pyroptosis in Mycobacteria Tuberculosis (Mtb) infected human macrophages. NBS Kontaktmøte 2019-01-24 - 2019-01-27
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PosterBeckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Ullmann, Sindre; Sætra, Ragnhild Sofie Ragnhildstveit; Marstad, Anne; Åsberg, Signe. (2019) Plasma membrane damage during Mycobacterium tuberculosis infection. The 4th Turning the Tide of Antimicrobial resistance meeting 2019-11-18 - 2019-11-20
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PosterUllmann, Sindre; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Sætra, Ragnhild Sofie Ragnhildstveit; Marstad, Anne; Åsberg, Signe. (2019) Catching the moment - Correlative Light Electron Microscopy (CLEM) to study hostpathogen interactions in single cells, at ultimate spatial and temporal resolutions. Conference on Molecular Mechanisms of Inflammation , Trondheim 2019-06-03 - 2019-06-06
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PosterHaug, Markus; Meås, Hany Zakaria; Beckwith, Marianne Sandvold; Louet, Claire; Ryan, Liv; Hu, Z.. (2019) Sensing of HIV-1 nucleic acids by TLR8 in human CD4+ T cells. 17th Congress of the International Union of Immunological Societies (IUIS) , Beijing 2019-10-19 - 2019-10-23
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Academic lectureMeås, Hany Zakaria; Haug, Markus; Beckwith, Marianne Sandvold; Louet, Claire; Ryan, Liv; Hu, Z.. (2019) Sensing of HIV-1 by TLR8 activates human T cells and reverses latency. International Conference on Molecular Mechanisms of Inflammation , Trondheim 2019-06-03 - 2019-06-06
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PosterUllmann, Sindre; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Sætra, Ragnhild Sofie Ragnhildstveit; Marstad, Anne; Strand, Trine Aakvik. (2019) Catching the moment - Correlative Light Electron Microscopy (CLEM) to study hostpathogen interactions in single cells, at ultimate spatial and temporal resolutions. The 4th Turning the Tide of Antimicrobial resistance meeting , Oslo 2019-11-18 - 2019-11-20
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PosterUllmann, Sindre; Sporsheim, Bjørnar; Egeberg, Kjartan Wøllo; Beckwith, Marianne Sandvold; Beckwith, Kai Sandvold; Sætra, Ragnhild Sofie Ragnhildstveit. (2019) Correlative light-electron microscopy investigations of single-cell dynamics in Mycobacteria tuberculosis infected human macrophages. Seeing is Believing - Imaging the Molecular Processes of Life , EMBL Heidelberg 2019-10-09 - 2019-10-12
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Gidon, Alexandre; Ullmann, Sindre; Sætra, Ragnhild Sofie Ragnhildstveit. (2019) Plasma membrane damage by Mycobacterium tuberculosis induce inflammasome activation and pyroptosis in host macrophages. International Symposium on Discovery of Actionable Targets in Infectious Diseases , Mangalore 2019-11-26 - 2019-11-26
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PosterMeås, Hany Zakaria; Haug, Markus; Beckwith, Marianne; Louet, Claire; Ryan, Liv; Landskron, Johannes. (2019) Sensing of HIV-1 by TLR8 activates human T cells and reverses Latency. Norsk Biokjemisk Selskap Kontaktmøte 2019 , Røros 2019-01-24 - 2019-01-27
2018
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PosterBeckwith, Marianne; Beckwith, Kai Sandvold; Ullmann, Sindre; Åsberg, Signe; Strand, Trine Aakvik; Flo, Trude Helen. (2018) Single-cell dynamics of pyroptosis in M. tuberculosis infected macrophages. EMBO workshop: The inflammasomes , Martinsried (Münich) 2018-09-25 - 2018-09-28
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PosterMeås, Hany Zakaria; Haug, Markus; Beckwith, Marianne; Louet, Claire; Ryan, Liv; Landskron, Johannes. (2018) Sensing of HIV-1 by TLR8 activates human T cells and reverses Latency. Keystone HIV and Co-Infections: Pathogenesis, Inflammation and Persistence (X8) , Whistler 2018-04-15 - 2018-04-19
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Academic lectureFlo, Trude Helen; Beckwith, Marianne; Meås, Hany Zakaria; Haug, Markus; Damås, Jan Kristian; Ryan, Liv. (2018) Sensing of HIV-1 by TLR8 activates human T cells and reverses latency. PhD defense , Oslo 2018-08-27 - 2018-08-28
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Academic lectureFlo, Trude Helen; Beckwith, Kai Sandvold; Beckwith, Marianne. (2018) Single-cell dynamics of pyroptosis in Mycobacterium tuberculosis infected macrophages. PhD defense 2018-10-24 - 2018-10-25
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Academic lectureFlo, Trude Helen; Beckwith, Marianne; Beckwith, Kai Sandvold; Ullmann, Sindre; Åsberg, Signe; Marstad, Anne. (2018) Single-cell dynamics of pyroptosis in Mycobacterium tuberculosis infected macrophages. 51st Annual Meeting Society for Leukocyte Biology & International Endotoxin and Innate Immunity Society , Chandler, Arizona 2018-10-14 - 2018-10-18
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Academic lectureFlo, Trude Helen; Beckwith, Marianne; Beckwith, Kai Sandvold. (2018) Single-cell dynamics of pyroptosis in Mycobacterium tuberculosis infected macrophages. TOLL2018: Editing Innate Immunity , Porto 2018-06-05 - 2018-06-10
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LectureBeckwith, Marianne. (2018) Tailor made aclar for correlative imaging by light microscopy and FIB-SEM tomography. EMBL seminar , Heidelberg 2018-06-07 - 2018-06-07
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PosterBeckwith, Kai Sandvold; Beckwith, Marianne; Ullmann, Sindre; Åsberg, Signe; Flo, Trude Helen. (2018) Single-cell dynamics of pyroptosis in M. Tuberculosis infected macrophages. EMBL Conference: From Images to Knowledge with ImageJ & Friends , Heidelberg 2018-12-06 - 2018-12-08
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LectureBeckwith, Kai Sandvold; Beckwith, Marianne; Ullmann, Sindre; Åsberg, Signe; Sætra, Ragnhild Sofie Ragnhildstveit; Strand, Trine Aakvik. (2018) Damage and repair of host cell membranes during M. Tuberculosis infection. EMBL seminar , Heidelberg 2018-12-06 - 2018-12-06
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Academic lectureBeckwith, Kai Sandvold; Beckwith, Marianne; Ullmann, Sindre; Åsberg, Signe; Strand, Trine Aakvik; Flo, Trude Helen. (2018) SINGLE-CELL DYNAMICS OF PYROPTOSIS IN M. TUBERCULOSIS INFECTED MACROPHAGES. Annual meeting Norwegian Research School for Infection biology and antimicrobials , Tromsø 2018-10-29 - 2018-10-30
2017
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Academic lectureFlo, Trude Helen; Beckwith, Marianne; Beckwith, Kai Sandvold. (2017) The cell biology of Mycobacterium tuberculosis induced inflammasome activation and killing of host macrophages. The 35th annual meeeting Norwegian Society for Immunology , Oslo 2017-11-17 -
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PosterBeckwith, Marianne; Beckwith, Kai Sandvold; Strand, Trine Aakvik; Flo, Trude Helen. (2017) In situ imaging of pyroptosis caused by Mycobacterium tuberculosis. Keystone X5: From Innate Immunity to Inflammatory Disease , Banff 2017-03-19 - 2017-03-23
2016
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Academic lectureBeckwith, Marianne. (2016) Mycobacteria and their intracellular hiding place. Fusjonsseminar NTNU , Trondheim 2016-11-10 - 2016-11-11
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Academic lectureBeckwith, Marianne. (2016) Corellative 3D light and electron microscopy - M. Avium-infected macrophages. SCANDEM 2016 , TRONDHEIM 2016-06-07 - 2016-06-10
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Academic lectureBeckwith, Marianne. (2016) Intracellular life in nanoscale 3D - Correlative imaging by light microscopy and FIB/SEM tomography. IBA forskerskole kick-off , Oslo 2016-11-14 - 2016-11-15
2014
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Academic lectureBeckwith, Marianne. (2014) Aclar microwells for Correlative Imaging of Mycobacterium infected Macrophages by CLSM and FIB/SEM. SCANDEM 2014 , Linköping 2014-06-09 - 2014-06-13
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Academic lectureBeckwith, Marianne. (2014) Correlative Imaging of Mycobacterium infected Macrophages by CLSM and FIB/SEM - A system for high resolution 3D investigation. CMIC seminar , Trondheim 2014-03-28 - 2014-03-28
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LectureBeckwith, Marianne. (2014) Du tror det når du får se det: en infeksjon i tre dimensjoner. Forsker grand prix Trondheim , Byscenen, Trondheim 2014-09-25 - 2014-09-25
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Academic lectureBeckwith, Marianne. (2014) Optimizing Correlative Imaging of Mycobacterium-infected Macrophages by CLSM and FIB/SEM. Norwegian Biochemical Society (NBS) Contact Meeting 2014 , Røros 2014-01-23 - 2014-01-26
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PosterBeckwith, Marianne; Beckwith, Kai Sandvold; Sikorski, Pawel; Flo, Trude Helen; Halaas, Øyvind. (2014) Correlative imaging by light microscopy and FIB/SEM tomography. NTNU NanoLab 10 Year Anniversary , Trondheim 2014-11-10 - 2014-11-11
2013
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LectureBeckwith, Marianne. (2013) Electron Microscopy Techniques for Biological Applications. Seminar on Biological TEM 2013-09-05 - 2013-09-05