Background and activities
Nanomedicines are extensively studied for in vivo therapeutic and diagnostic applications. Although several nanodrugs have been clinically approved and many are in clinical trials, the medical potential of these agents remains to be fully exploited. There are multiple reasons for this unsatisfactory utilization, but an important underlying issue is incomplete understanding of nanoparticle in vivo applications.
In my group, we attempt to define the gaps in the knowledge about nanoparticle ‘in vivo behavior’, and develop and use a range of methods to address these issues. At the heart of our effort lie various in vivo imaging modalities, like whole animal optical imaging, magnetic resonance imaging, and intravital microscopy. Especially intravital microscopy has proven extremely powerful for studying nanoparticle drug release and tumor targeting kinetics, as well as nanoparticle interactions with various cells in real-time. For ex vivo analysis, we have implemented state-of-the-art flow cytometry approaches allowing us to map interactions of administered nanoparticles with numerous cell types. Using this combination of in vivo and ex vivo methods, we are now discovering pivotal roles for various cells of the immune system in nanoparticle in vivo applications.
Using the new and exciting insights we gained over recent years, we are aiming to develop novel nanotherapeutic approaches with a focus on cancer. At the same time, we continue to increase our understanding of nanoparticles’ complex ‘in vivo behavior’.
Scientific, academic and artistic work
A selection of recent journal publications, artistic productions, books, including book and report excerpts. See all publications in the database
- (2021) Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery. European journal of pharmaceutics and biopharmaceutics. vol. 163.
- (2021) Cyclic Arginine–Glycine–Aspartate-Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes. Advanced Science. vol. 8 (13).
- (2020) In vitro and in vivo evaluation of organic solvent-free injectable melatonin nanoformulations. European journal of pharmaceutics and biopharmaceutics. vol. 152.
- (2020) Trained Immunity-Promoting Nanobiologic Therapy Suppresses Tumor Growth and Potentiates Checkpoint Inhibition. Cell. vol. 183 (3).
- (2020) Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging. Nature Nanotechnology. vol. 15 (5).
- (2020) Tumor Targeting by αvβ3-Integrin-Specific Lipid Nanoparticles Occurs via Phagocyte Hitchhiking. ACS Nano. vol. 14 (7).
- (2019) Mononuclear but Not Polymorphonuclear Phagocyte Depletion Increases Circulation Times and Improves Mammary Tumor-Homing Efficiency of Donor Bone Marrow-Derived Monocytes. Cancers. vol. 11 (11).
- (2019) Simple and robust intravital microscopy procedures in hybrid TIE2GFP-BALB/c transgenic mice. Molecular Imaging and Biology. vol. 22 (3).
- (2018) Nanoparticle Ligand-Decoration Procedures Affect in Vivo Interactions with Immune Cells. Molecular Pharmaceutics. vol. 15 (12).
- (2018) Translating nanomedicines: Thinking beyond materials? A young investigator's reply to `The Novelty Bubble’. Journal of Controlled Release. vol. 290.
- (2017) Integrating nanomedicine and imaging. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences. vol. 375 (2107).
- (2017) Real-Time Monitoring of Nanoparticle Formation by FRET Imaging. Angewandte Chemie International Edition. vol. 56 (11).
- (2017) Synthesis of gadolinium oxide nanodisks and gadolinium doped iron oxide nanoparticles for MR contrast agents. Journal of materials chemistry. B. vol. 5 (3).
- (2016) L-DOPA-Coated Manganese Oxide Nanoparticles as Dual MRI Contrast Agents and Drug-Delivery Vehicles. Small. vol. 12 (3).
- (2016) Labeling nanoparticles: Dye leakage and altered cellular uptake. Cytometry Part A. vol. 91 (8).
- (2016) Augmenting drug-carrier compatibility improves tumour nanotherapy efficacy. Nature Communications. vol. 7.
- (2015) The effects of oil-in-water nanoemulsion polyethylene glycol surface density on intracellular stability, pharmacokinetics, and biodistribution in tumor bearing mice. Pharmaceutical Research. vol. 32 (4).
- (2015) Transverse relaxivity of iron oxide nanocrystals clustered in nanoemulsions: Experiment and theory. Magnetic Resonance in Medicine. vol. 74 (3).
- (2015) Nanoparticle-stabilized microbubbles for multimodal imaging and drug delivery. Contrast Media & Molecular Imaging. vol. 10 (5).
- (2015) Nanoparticle delivery to the brain - By focused ultrasound and self-assembled nanoparticle-stabilized microbubbles. Journal of Controlled Release. vol. 220.