TFY4265 - Biophysical Micromethods


Examination arrangement

Examination arrangement: Aggregate score
Grade: Letter grades

Evaluation Weighting Duration Grade deviation Examination aids
Oral exam 50/100 1 hours E
Presentation 50/100

Course content

The course gives an introduction to different types of instrumentation that are important for studies of biological macromolecules, cells, and other soft materials. The course provides an understanding of the function of the components that the instrumentation consists of, as well as a theoretical and practical understanding of how to operate the instrument, including calibration procedures and maintenance.

Learning outcome


The candidate should have knowledge about

  • properties of optical radiation and key interactions between light and matter that are relevant for the techniques presented in the course.
  • basic geometrical optics and wave optics.
  • the most central optical imaging techniques for biological samples (bright field microscopy and various contrast techniques, fluorescence microscopy, multiphoton microscopy, superresolution microscopy and others.)
  • other optical techniques used for biological samples like FCS, FRAP and FRET and others.
  • preparation of samples for optical techniques, including various staining procedures.
  • interactions between electrons and biological materials.
  • various electron microscopy techniques like SEM, TEM, STEM and analytic methods.
  • preparation of samples for electron microscopy.


The candidate should have skills within the use of common microscopy techniques like bright field microscopy with various contrast mechanisms and confocal microscopy.

General competence:

The candidate should through the course develop competency in

  • searching and reading scientific literature.
  • oral presentation of scientific studies.
  • writing scientific texts.
  • collaboration.
  • preparation of scientific poster.

Learning methods and activities

Lectures and laboratory exercises. Expected workload in the course is 225 hours.

Compulsory assignments

  • Laboratory exercises

Further on evaluation

The total grade in this course is based on an oral exam (50%) and a presentation of a scientific paper (50%). Lectures in English.

Course materials

Various written texts (book chapters, compendium, papers).

Credit reductions

Course code Reduction From To
SIF4071 7.5
FY8906 7.5 AUTUMN 2010
FY8410 5.0 AUTUMN 2011
More on the course



Version: 1
Credits:  7.5 SP
Study level: Second degree level


Term no.: 1
Teaching semester:  AUTUMN 2024

Language of instruction: English

Location: Trondheim

Subject area(s)
  • Biophysics and Medical Technology
  • Physics
Contact information
Course coordinator:

Department with academic responsibility
Department of Physics


Examination arrangement: Aggregate score

Term Status code Evaluation Weighting Examination aids Date Time Examination system Room *
Autumn ORD Presentation 50/100 INSPERA
Room Building Number of candidates
Autumn ORD Oral exam 50/100 E
Room Building Number of candidates
Summer UTS Oral exam 50/100 E
Room Building Number of candidates
  • * The location (room) for a written examination is published 3 days before examination date. If more than one room is listed, you will find your room at Studentweb.

For more information regarding registration for examination and examination procedures, see "Innsida - Exams"

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