Anne Mari A. Rokstad

About

Inflammation, the body’s natural process in early defense of foreign invaders or in endogenous processes as wound healing, is increasingly recognized as an essential player in disease. Behind an inflammatory process are cellular and protein players communicating in complex ways. Depending on the disturbance, different cellular or protein might be involved. To understand these systems could help us develop new treatment points and strategies in the fights against various diseases for improved health and wellbeing.

One major focus of my research is to determine the inflammatory mechanisms of exogenous and endogenous materials. More explained, the exogenous materials are externally derived as transplantation devices, wound healing materials or nano- and microparticles. The endogenous materials are produced by the body itself and often involved in promoting disease. An example is cholesterol crystals formed in atherosclerotic plaques. Recently we demonstrated that cholesterol crystals are promoting thromboinflammation, and thus are active players in thrombosis. Despite different materials, they all impact the immune system through common and specific mechanistic pathways. By revealing the underlying mechanisms new treatment possibilities can be determined. The overall aim by this research is understanding the inflammatory/immune system at the intersection of materials exposure and use this knowledge for improving our health.

Inflammation is an underlying factor in obesity related disease, and the immune system and metabolic system are interrelated. Revealing connections between obesity and inflammation could open for new understanding in treatment. Connected to Centre for Obesity and Innovation (ObeCe) at St. Olav’s hospital, we explore how calorie restriction diet/weight reduction impact low-degree inflammation and thromboinflammation.

I am a part of the NTNU health team "Tailored biomaterials for reduced immune responses" that are currently developing/exploring new materials alongside mechanisms understanding. I am also a part of a global network of researchers with the overall goal of developing long-term sustainable (fibrosis-free) biodevices in cell therapy for Type 1 diabetes.

Publications

2022

Coron, Abba Elizabeth; Fonseca, Davi de Miranda; Sharma, Animesh; Slupphaug, Geir; Strand, Berit Løkensgard; Rokstad, Anne Mari A.. (2022) MS-proteomics provides insight into the host responses towards alginate microspheres. Materials Today Bio
Academic article
Syanda, Adam M.; Kringstad, Vera I.; Blackford, Samuel J.I.; Kjesbu, Joachim Sebastian; Ng, Soon Seng; Ma, Liang. (2022) Sulfated alginate reduces pericapsular fibrotic overgrowth on encapsulated cGMP-compliant hPSC-hepatocytes in mice. Frontiers in Bioengineering and Biotechnology
Academic article

2021

Coron, Abba E.; Kjesbu, Joachim Sebastian; Kjærnsmo, Fredrikke; Oberholzer, José; Rokstad, Anne Mari A.; Strand, Berit Løkensgard. (2021) Pericapsular fibrotic overgrowth mitigated in immunocompetent mice through microbead formulations based on sulfated or intermediate G alginates. Acta Biomaterialia
Academic article

2020

Niyonzima, Nathalie; Bakke, Siril Skaret; Gregersen, Ida; Holm, Sverre; Sandanger, Øystein; Orrem, Hilde Lang. (2020) Cholesterol crystals use complement to increase NLRP3 signaling pathways in coronary and carotid atherosclerosis. EBioMedicine
Academic article

2019

Gravastrand, Caroline S.; Steinkjer, Bjørg; Halvorsen, Bente; Landsem, Anne; Skjelland, Mona; Jacobsen, Eva Astrid. (2019) Cholesterol Crystals Induce coagulation Activation through Complement-Dependent Expression of Monocytic Tissue Factor. Journal of Immunology
Academic article
Nordli, Henriette R.; Pukstad, Brita; Chinga-Carrasco, Gary; Rokstad, Anne Mari. (2019) Ultrapure Wood Nanocellulose - Assessments of Coagulation and Initial Inflammation Potential. ACS Applied Bio Materials (AABM)
Academic article

2018

Kopplin, Georg; Rokstad, Anne Mari; Mélida, Hugo; Bulone, Vincent; Skjåk-Bræk, Gudmund; Aachmann, Finn Lillelund. (2018) Structural characterization of fucoidan from Laminaria hyperborea: Assessment of coagulation and inflammatory properties and their structure−function relationship. ACS Applied Bio Materials (AABM)
Academic article
Bochenek, Matthew A.; Veiseh, Omid; Vegas, Arturo J.; McGarrigle, James J.; Qi, Meirigeng; Marchese, Enza. (2018) Alginate encapsulation as long-term immune protection of allogeneic pancreatic islet cells transplanted into the omental bursa of macaques. Nature Biomedical Engineering
Academic article

2017

Vaithilingam, Vijayaganapathy; Steinkjer, Bjørg; Ryan, Liv; Larsson, Rolf; Tuch, Bernard E.; Oberholzer, José. (2017) In Vitro and In Vivo Biocompatibility Evaluation of Polyallylamine and Macromolecular Heparin Conjugates Modified Alginate Microbeads. Scientific Reports
Academic article
Gravastrand, Caroline; Hamad, Shamal; Fure, Hilde; Steinkjer, Bjørg; Ryan, Liv; Oberholzer, José. (2017) Alginate microbeads are coagulation compatible, while alginate microcapsules activate coagulation secondary to complement or directly through FXII. Acta Biomaterialia
Academic article
Wolf-Grosse, Susann; Rokstad, Anne Mari; Ali, Syed; Lambris, JD; Mollnes, Tom Eirik; Nilsen, Asbjørn Magne. (2017) Iron oxide nanoparticles induce cytokine secretion in a complement-dependent manner in a human whole blood model. International Journal of Nanomedicine
Academic article

2016

Ørning, Mathias Pontus; Hoem, Kine Samset; Coron, Abba Elizabeth; Skjåk-Bræk, Gudmund; Mollnes, Tom Eirik; Brekke, Ole Lars. (2016) Alginate microsphere compositions dictate different mechanisms of complement activation with consequences for cytokine release and leukocyte activation. Journal of Controlled Release
Academic article
Arlov, Øystein; Skjåk-Bræk, Gudmund; Rokstad, Anne Mari. (2016) Sulfated alginate microspheres associate with factor H and dampen the inflammatory cytokine response. Acta Biomaterialia
Academic article
Nordli, Henriette R.; Chinga-Carrasco, Gary; Rokstad, Anne Mari; Pukstad, Brita. (2016) Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells. Carbohydrate Polymers
Academic article

2015

Sandvig, Ioanna; Karstensen, Kristin; Rokstad, Anne Mari; Aachmann, Finn Lillelund; Formo, Kjetil Ødegård; Sandvig, Axel. (2015) RGD-peptide modified alginate by a chemoenzymatic strategy for tissue engineering applications. Journal of Biomedical Materials Research. Part A
Academic article
Niyonzima, Nathalie; Samstad, Eivind; Aune, Marie Hjelmseth; Ryan, Liv; Bakke, Siril Skaret; Rokstad, Anne Mari. (2015) Reconstituted high-density lipoprotein attenuates cholesterol crystal-induced inflammatory responses by reducing complement activation. Journal of Immunology
Academic article

2014

Rokstad, Anne Mari; Lacík, Igor; DeVos, Paul; Strand, Berit Løkensgard. (2014) Advances in biocompatibility and physico-chemical characterization of microspheres for cell encapsulation. Advanced Drug Delivery Reviews
Academic literature review

2013

Hals, Ingrid Katrin; Rokstad, Anne Mari; Strand, Berit Løkensgard; Oberholzer, José; Grill, Valdemar. (2013) Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia. Experimental Diabetes Research
Academic article
Rokstad, Anne Mari; Strand, Berit Løkensgard; Espevik, Terje; Mollnes, Tom Eirik. (2013) Biocompatibility and Biotolerability Assessment of Microspheres Using a Whole Blood Model. Micro and Nanosystems
Academic article
Rokstad, Anne Mari; Brekke, Ole Lars; Steinkjer, Bjørg; Ryan, Liv; Kolláriková, Gabriela; Strand, Berit Løkensgard. (2013) The induction of cytokines by polycation containing microspheres by a complement dependent mechanism. Biomaterials
Academic article

2012

Rokstad, Anne Mari; Brekke, Ole Lars; Steinkjer, Bjørg; Ryan, Liv; Kolláriková, Gabriela; Lambris, John D.. (2012) Poly-cation containing alginate microcapsules induce cytokines by a complement-dependent mechanism. Immunobiology
Abstract
Rokstad, Anne Mari; Gustafsson, Björn; Espevik, Terje; Bakke, Ingunn; Pfragner, Roswitha; Svejda, Bernhard. (2012) Microencapsulation of small intestinal neuroendocrine neoplasm cells for tumor model studies. Cancer Science
Academic article

2011

Rokstad, Anne Mari; Brekke, Ole Lars; Steinkjer, Bjørg; Ryan, Liv; Kolláriková, Gabriela; Strand, Berit Løkensgard. (2011) Alginate microbeads are complement compatible, in contrast to polycation containing microcapsules, as revealed in a human whole blood model. Acta Biomaterialia
Academic article

Journal publications

Coron, Abba Elizabeth; Fonseca, Davi de Miranda; Sharma, Animesh; Slupphaug, Geir; Strand, Berit Løkensgard; Rokstad, Anne Mari A.. (2022) MS-proteomics provides insight into the host responses towards alginate microspheres. Materials Today Bio
Academic article
Syanda, Adam M.; Kringstad, Vera I.; Blackford, Samuel J.I.; Kjesbu, Joachim Sebastian; Ng, Soon Seng; Ma, Liang. (2022) Sulfated alginate reduces pericapsular fibrotic overgrowth on encapsulated cGMP-compliant hPSC-hepatocytes in mice. Frontiers in Bioengineering and Biotechnology
Academic article
Coron, Abba E.; Kjesbu, Joachim Sebastian; Kjærnsmo, Fredrikke; Oberholzer, José; Rokstad, Anne Mari A.; Strand, Berit Løkensgard. (2021) Pericapsular fibrotic overgrowth mitigated in immunocompetent mice through microbead formulations based on sulfated or intermediate G alginates. Acta Biomaterialia
Academic article
Niyonzima, Nathalie; Bakke, Siril Skaret; Gregersen, Ida; Holm, Sverre; Sandanger, Øystein; Orrem, Hilde Lang. (2020) Cholesterol crystals use complement to increase NLRP3 signaling pathways in coronary and carotid atherosclerosis. EBioMedicine
Academic article
Gravastrand, Caroline S.; Steinkjer, Bjørg; Halvorsen, Bente; Landsem, Anne; Skjelland, Mona; Jacobsen, Eva Astrid. (2019) Cholesterol Crystals Induce coagulation Activation through Complement-Dependent Expression of Monocytic Tissue Factor. Journal of Immunology
Academic article
Nordli, Henriette R.; Pukstad, Brita; Chinga-Carrasco, Gary; Rokstad, Anne Mari. (2019) Ultrapure Wood Nanocellulose - Assessments of Coagulation and Initial Inflammation Potential. ACS Applied Bio Materials (AABM)
Academic article
Kopplin, Georg; Rokstad, Anne Mari; Mélida, Hugo; Bulone, Vincent; Skjåk-Bræk, Gudmund; Aachmann, Finn Lillelund. (2018) Structural characterization of fucoidan from Laminaria hyperborea: Assessment of coagulation and inflammatory properties and their structure−function relationship. ACS Applied Bio Materials (AABM)
Academic article
Bochenek, Matthew A.; Veiseh, Omid; Vegas, Arturo J.; McGarrigle, James J.; Qi, Meirigeng; Marchese, Enza. (2018) Alginate encapsulation as long-term immune protection of allogeneic pancreatic islet cells transplanted into the omental bursa of macaques. Nature Biomedical Engineering
Academic article
Vaithilingam, Vijayaganapathy; Steinkjer, Bjørg; Ryan, Liv; Larsson, Rolf; Tuch, Bernard E.; Oberholzer, José. (2017) In Vitro and In Vivo Biocompatibility Evaluation of Polyallylamine and Macromolecular Heparin Conjugates Modified Alginate Microbeads. Scientific Reports
Academic article
Gravastrand, Caroline; Hamad, Shamal; Fure, Hilde; Steinkjer, Bjørg; Ryan, Liv; Oberholzer, José. (2017) Alginate microbeads are coagulation compatible, while alginate microcapsules activate coagulation secondary to complement or directly through FXII. Acta Biomaterialia
Academic article
Wolf-Grosse, Susann; Rokstad, Anne Mari; Ali, Syed; Lambris, JD; Mollnes, Tom Eirik; Nilsen, Asbjørn Magne. (2017) Iron oxide nanoparticles induce cytokine secretion in a complement-dependent manner in a human whole blood model. International Journal of Nanomedicine
Academic article
Ørning, Mathias Pontus; Hoem, Kine Samset; Coron, Abba Elizabeth; Skjåk-Bræk, Gudmund; Mollnes, Tom Eirik; Brekke, Ole Lars. (2016) Alginate microsphere compositions dictate different mechanisms of complement activation with consequences for cytokine release and leukocyte activation. Journal of Controlled Release
Academic article
Arlov, Øystein; Skjåk-Bræk, Gudmund; Rokstad, Anne Mari. (2016) Sulfated alginate microspheres associate with factor H and dampen the inflammatory cytokine response. Acta Biomaterialia
Academic article
Nordli, Henriette R.; Chinga-Carrasco, Gary; Rokstad, Anne Mari; Pukstad, Brita. (2016) Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells. Carbohydrate Polymers
Academic article
Sandvig, Ioanna; Karstensen, Kristin; Rokstad, Anne Mari; Aachmann, Finn Lillelund; Formo, Kjetil Ødegård; Sandvig, Axel. (2015) RGD-peptide modified alginate by a chemoenzymatic strategy for tissue engineering applications. Journal of Biomedical Materials Research. Part A
Academic article
Niyonzima, Nathalie; Samstad, Eivind; Aune, Marie Hjelmseth; Ryan, Liv; Bakke, Siril Skaret; Rokstad, Anne Mari. (2015) Reconstituted high-density lipoprotein attenuates cholesterol crystal-induced inflammatory responses by reducing complement activation. Journal of Immunology
Academic article
Rokstad, Anne Mari; Lacík, Igor; DeVos, Paul; Strand, Berit Løkensgard. (2014) Advances in biocompatibility and physico-chemical characterization of microspheres for cell encapsulation. Advanced Drug Delivery Reviews
Academic literature review
Hals, Ingrid Katrin; Rokstad, Anne Mari; Strand, Berit Løkensgard; Oberholzer, José; Grill, Valdemar. (2013) Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia. Experimental Diabetes Research
Academic article
Rokstad, Anne Mari; Strand, Berit Løkensgard; Espevik, Terje; Mollnes, Tom Eirik. (2013) Biocompatibility and Biotolerability Assessment of Microspheres Using a Whole Blood Model. Micro and Nanosystems
Academic article
Rokstad, Anne Mari; Brekke, Ole Lars; Steinkjer, Bjørg; Ryan, Liv; Kolláriková, Gabriela; Strand, Berit Løkensgard. (2013) The induction of cytokines by polycation containing microspheres by a complement dependent mechanism. Biomaterials
Academic article
Rokstad, Anne Mari; Brekke, Ole Lars; Steinkjer, Bjørg; Ryan, Liv; Kolláriková, Gabriela; Lambris, John D.. (2012) Poly-cation containing alginate microcapsules induce cytokines by a complement-dependent mechanism. Immunobiology
Abstract
Rokstad, Anne Mari; Gustafsson, Björn; Espevik, Terje; Bakke, Ingunn; Pfragner, Roswitha; Svejda, Bernhard. (2012) Microencapsulation of small intestinal neuroendocrine neoplasm cells for tumor model studies. Cancer Science
Academic article
Rokstad, Anne Mari; Brekke, Ole Lars; Steinkjer, Bjørg; Ryan, Liv; Kolláriková, Gabriela; Strand, Berit Løkensgard. (2011) Alginate microbeads are complement compatible, in contrast to polycation containing microcapsules, as revealed in a human whole blood model. Acta Biomaterialia
Academic article

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