Background and activities
About the lab
The overarching goal of research in the Oudhoff lab is to define the cellular and molecular mechanisms that control infection, regeneration, and inflammation in the digestive system. The digestive tract is responsible for absorption of nutrients and water, but at the same time it has a crucial role in acting as a barrier to the external environment. The barrier function is complicated by the requirement to simultaneously be able to respond appropriately to dangerous pathogens, and remain tolerant to innocuous antigens like commensal organisms and food. Dysregulation of this equilibrium, either by exposure to infection and/or chemicals or by genetic predisposition can lead to (chronic) diseases such as inflammatory bowel diseases (IBDs), food allergies, and cancer. Understanding the molecular and cellular principles underlying development and homeostasis and disease provide important means for identifying novel therapeutics.
I am currently a group leader at the Centre of Molecular Inflammation Research (CEMIR, www.ntnu.edu/cemir) which is hosted by the Faculty of Medicine at the Norwegian University of Science and Technology (NTNU) in Trondheim. After completing my MSc degree in Biology in 2006, I did my PhD at the Department of Oral Biochemistry (VU University, Amsterdam) under supervision of Dr. Enno Veerman where I studied salivary peptides in wound healing. In 2011, I joined the Mucosal Immunology lab of Dr. Colby Zaph at UBC in Vancouver (www.zaphlab.com, go see the profile pictures!).
Judson RN, Quarta M, Oudhoff MJ, Soliman H, Yi L, Chang CK, Loi G, Vander Werff R, Cait A, Hamer M, Blonigan J, Paine P, Doan LTN, Groppa E, He W, Su L, Zhang RH, Xu P, Eisner C, Low M, Barta I, Lewis CB, Zaph C, Karimi MM, Rando TA, Rossi FM. Cell Stem Cell. 2018 Feb 1;22(2):177-190.e7. doi: 10.1016/j.stem.2017.12.010. Epub 2018 Jan 25.
- Preview by Fujita & Crist: Slow Your Roll: Inhibiting SETD7 Activity Permits Ex Vivo Expansion of Muscle Stem Cells.
Gómez-Escudero J, Moreno V, Martín-Alonso M, Hernández-Riquer MV, Feinberg T, Colmenar Á, Calvo E, Camafeita E, Martínez F, Oudhoff MJ, Weiss SJ, Arroyo AG 2017. E-cadherin cleavage by MT2-MMP regulates apical junctional signaling and epithelial homeostasis in the intestine. J Cell Sci. . 2017 Dec 1;130(23):4013-4027 Cover of the issue by Dr. Martín-Alonso
- Oudhoff, MJ*, Antignano, F*, Chenery, AL, Burrows, K, Redpath, SA, Braam, MJS, Perona-Wright, GP, Zaph, C 2016. Intestinal Epithelial Cell-Intrinsic Deletion of Setd7 Identifies Role for Developmental Pathways in Immunity to Helminth Infection. PLoS Pathogens 2016 Sep 6;12(9):e1005876
- Antignano F, Braam MJS, Hughes MR, Chenery A, Burrows K, Gold MJ, Oudhoff MJ, Rattray D, Halim T, Cait AM, Takei F, Rossi FMV, McNagny KM, Zaph C. 2016. G9a regulates group 2 innate lymphoid cell development by repressing the group 3 innate lymphoid cell program. J. Exp. Med. 213 (7): 1153-62
- Oudhoff MJ, Braam MJS, Freeman SA, Wong D, Rattray DG, Wang J, Antignano F, Snyder K, Refaeli I, Hughes MR, McNagny KM, Gold MR, Arrowsmith CH, Sato T, Rossi FMV, Tatlock JH, Owen DR, Brown PJ, Zaph C. 2016. SETD7 controls intestinal regeneration and tumorigenesis by regulating Wnt/b-Catenin and Hippo/YAP signaling. Dev. Cell 37 (1): 47–57.
- Barsyte-Lovejoy D*, Li F*, Oudhoff MJ*, Tatlock JH*, Dong A, Zeng H, Wu H, Freeman SA, Schapira M, Senisterra GA, Kuznetsova E, Marcellus R, Allali-Hassani A, Kennedy S, Lambert J-P, Couzens AL, Aman A, Gingras A-C, Al-Awar R, Fish PV, Gerstenberger BS, Roberts L, Benn CL, Grimley RL, Braam MJS, Rossi FMV, Sudol M, Brown PJ, Bunnage ME, Owen DR, Zaph C, Vedadi M, Arrowsmith CH. 2014. (R)-PFI-2 is a potent and selective inhibitor of SETD7 methyltransferase activity in cells. PNAS 111 (35): 12853–12858. *equal contribution
- Antignano F, Burrows K, Hughes, ML, Han JM, Kron KJ, Penrod NM, Oudhoff MJ, Wang SKH, Min PH, Gold MJ, Chenery A, Braam MJS, Fung TC, Rossi FMV, McNagny KM, Arrowsmith CH, Lupien M, Levings MK, Zaph C. 2014. Methyltransferase G9A regulates T cell differentiation during murine intestinal inflammation. J. Clin. Invest. 124 (5):1945–1955.
- Mullaly SC, Oudhoff MJ, Min PH, Burrows, K, Antignano F, Rattray DG, Chenery A, McNagny KM, Ziltener HJ, Zaph C. 2013. Requirement for Core 2 O-Glycans for Optimal Resistance to Helminth Infection. PLoS ONE 8 (3): e60124. doi: 10.1371/journal.pone.0060124.
- Oudhoff MJ, Freeman SA, Couzens AL, Antignano F, Min PH, Northrop JP, Burrows K, Chenery A, Lehnertz B, Barsyte-Lovejoy D, Vedadi M, Arrowsmith CH, Nishina H, Gold MR, Rossi FMV, Gingras A-C, Zaph C. 2013. Regulation of the Hippo pathway through Set7-dependent methylation of Yap. Dev. Cell 26 (2): 188–194.
- Bron JL, Mulder HW, Vonk LA, Doulabi DZ, Oudhoff MJ, Smit TH (2012). Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement. J. Mat. Sci.: Mat. in Med. 23(3): 813-21
- Bolscher JG*, Oudhoff MJ*, Nazmi K, Antos JM, Guimareas CP, Spooner E, Haney EF, Garcia Vallejo JJ, Vogel HJ, van ‘t Hof W, Ploegh HL, Veerman EC (2011). Sortase A as a tool for high-yield histatin cyclization. FASEB J. 25(8): 2650-8 *equal contribution
- Oudhoff MJ, Blaauboer ME, Nazmi K, Scheres N, Bolscher JG, Veerman EC (2010). The role of salivary histatin and the human Cathelicidin LL-37 in wound healing and innate immunity. Biol. Chem. 391(5): 541-48
- Oudhoff MJ, Kroeze KL, Nazmi K, van den Keijbus PA, van 't Hof W, Fernandez-Borja M, Hordijk PL, Gibbs S, Bolscher JG, Veerman EC (2009). Structure-activity analysis of histatin, a potent wound healing peptide from human saliva: cyclization of histatin potentiates molar activity 1,000-fold. Faseb J. 23(11):3928-35.
- Oudhoff MJ, van den Keijbus PA, Kroeze KL, Nazmi K, Gibbs S, Bolscher JG, Veerman EC (2009). Histatins enhance wound closure with oral and non-oral cells. J. Dent. Res. 88(9):846-50.
- Oudhoff MJ, Bolscher JG, Nazmi K, Kalay H, van 't Hof W, Amerongen AV, Veerman EC (2008). Histatins are the major wound-closure stimulating factors in human saliva as identified in a cell culture assay. Faseb J. 22(11):3805-12.
- de Jong MA*, de Witte L*, Oudhoff MJ, Gringhuis SI, Gallay P, Geijtenbeek TB (2008). TNF-alpha and TLR agonists increase susceptibility to HIV-1 transmission by human Langerhans cells ex vivo. J. Clin. Invest. 118(10):3440-52. *equal contribution