Qiangwei Zhang
About
Techniques and skills: Molecular cloning of DNA constructs for both generating transgenic mouse lines and making recombinant AAV vectors. Design and generate in situ hybridization RNA probes. Characterization the gene expression using in situ hybridization (ISH) and immunohistochemical methods. Mammalian cell culture and AAV viral vector preparation. Cryopreserving transgenic mouse line by slow freezing embryos, rederivation of transfeneic mouse lines by transfering embyos into a foster mother mouse. Mouse vasectomy sugeries. Lab purchasing, mouse colony management. Health, Safety and Environment (HSE) responsibilities. Retro-obital injections in mice. Animal experiment support including breeding experimental animals, tissue sampling, ear tagging, PCR-based genotyping, perfusions etc.
Publications
2018
-
Rowland, David Clayton;
Obenhaus, Horst-Andreas;
Skytøen, Emilie;
Zhang, Qiangwei;
Kentros, Clifford;
Moser, Edvard Ingjald.
(2018)
Functional properties of stellate cells in medial entorhinal cortex layer II.
.
eLIFE
Academic article
Journal publications
-
Rowland, David Clayton;
Obenhaus, Horst-Andreas;
Skytøen, Emilie;
Zhang, Qiangwei;
Kentros, Clifford;
Moser, Edvard Ingjald.
(2018)
Functional properties of stellate cells in medial entorhinal cortex layer II.
.
eLIFE
Academic article