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Språkvelger

Norsk

Ralph Kissen

Ralph Kissen

overingeniør
Department of Biology

ralph.kissen@ntnu.no
+4773596191 DU1-181 Realfagbygget Gløshaugen, Trondheim
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About Publications

About

 

Tasks

  • research support for the Cell and Molecular Biology section
  • technical/administrative tasks (lab responsibility, operation of GMO-lab and analytical equipment, bookitlab, LN2)
  • contribute to teaching in cell and molecular biology
  • train master students on instruments and in techniques

 

Skills

  • extraction and analysis of DNA, RNA and proteins
  • extraction of metabolites and analysis by GC-MS and HPLC
  • expression of recombinant proteins in Escherichia coli, purification and functional characterization (immunoassay, enzyme assay)
  • work with plants:
    • grow in vitro (medium, hydroponics) and on soil
    • transform with Agrobacterium tumefaciens
    • genome editing (CRISPR/Cas9)
    • generate, screen and characterize mutants
    • assays with biotic and abiotic stress factors
  • work with diatoms (Phaeodactylum tricornutum): 
    • grow and maintain kultures
    • transform by biolistics and conjugation
    • genome editing (CRISPR/Cas9)
    • screen og characterize mutant lines

 

Publications

  • Chronological
  • By category
  • See all publications in Cristin

2021

  • Ahuja, Ishita; Kissen, Ralph; Hoang, Linh; Sporsheim, Bjørnar; Halle, Kari Krizak; Wolff, Silje Aase; Ahmad, Samina Jam Nazeer; Ahmad, Jam Nazeer; Bones, Atle M.. (2021) The imaging of guard cells of thioglucosidase (tgg) mutants of Arabidopsis further links plant chemical defence systems with physical defence barriers. Cells. volum 10 (2).
    Academic article

2020

  • Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn; Bones, Atle M.. (2020) Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker. Biotechnology Reports. volum 28.
    Academic article
  • Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn; Bones, Atle M.. (2020) Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker. Biotechnology Reports. volum 28.
    Academic article
  • Ting, Hieng Ming; Cheah, Boon Huat; Chen, Yu-Cheng; Yeh, Pei-Min; Cheng, Piu-Ching; Yeo, Freddy Kuok San; Vie, Ane Kjersti; Rohloff, Jens; Winge, Per; Bones, Atle M.; Kissen, Ralph. (2020) The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses. Frontiers in Plant Science. volum 11.
    Academic article

2019

  • Sharma, Amit Kumar; Mühlroth, Alice; Jouhet, Juliette; Maréchal, Eric; Alipanah, Leila; Kissen, Ralph; Brembu, Tore; Bones, Atle M.; Winge, Per. (2019) The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae. New Phytologist. volum 225 (6).
    Academic article

2018

  • Ruta, Lavinia L; Banu, Melania A.; Neagoe, Aurora D; Kissen, Ralph; Bones, Atle M.; Farcasanu, Ileana C.. (2018) Accumulation of (Ag(I) by Saccharomyces cereviseae cells expressing plant metallothioneins. Cells. volum 7 (12).
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2018) Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate. Scientific Reports. volum 8 (1).
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2018) Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases in Arabidopsis thaliana. Frontiers in Plant Science. volum 9.
    Academic article

2017

  • Ruta, Lavinia Liliana; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora Daniela; Petrescu, Andrei José; Bones, Atle M.; Farcasanu, Ileana Cornelia. (2017) Heavy Metal Accumulation by Saccharomyces cerevisiae Cells Armed with Metal Binding Hexapeptides Targeted to the Inner Face of the Plasma Membrane. Applied Microbiology and Biotechnology. volum 101 (14).
    Academic article
  • Ruta, Lavinia Liliana; Lin, Ya-Fen; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora Daniela; Ghenea, Simona; Bones, Atle M.; Farcasanu, Ileana Cornelia. (2017) Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation. PLOS ONE. volum 12 (5).
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2017) Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on their Side Chain Structure. International Journal of Molecular Sciences. volum 18 (11).
    Academic article

2016

  • Kissen, Ralph; Eberl, Franziska; Winge, Per; Uleberg, Eivind; Martinussen, Inger; Bones, Atle M.. (2016) Effect of growth temperature on glucosinolate profiles in Arabidopsis thaliana accessions. Phytochemistry. volum 130.
    Academic article
  • Kissen, Ralph; Øverby, Anders; Winge, Per; Bones, Atle M.. (2016) Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana. BMC Genomics. volum 17.
    Academic article

2015

  • Bones, Atle M.; Hara, M; Rossiter, JT; Kissen, Ralph. (2015) Physiology and cellular mechanisms of isothiocyanates and other glucosinolate degradation products in plants. Frontiers in Plant Science.
    Editorial

2012

  • Ahuja, Ishita; Kissen, Ralph; Bones, Atle M.. (2012) Phytoalexins in defense against pathogens. Trends in Plant Science. volum 17 (2).
    Scientific translation/review
  • Kissen, Ralph; Hyldbakk, Elisabeth; Wang, Chia-Wei Vivian; Sørmo, Christopher; Rossiter, John T.; Bones, Atle M.. (2012) Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana. Plant Molecular Biology. volum 78 (4-5).
    Academic article
  • Kong, Xiang Yi; Kissen, Ralph; Bones, Atle M.. (2012) Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: Dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions. Phytochemistry. volum 84.
    Academic article

2010

  • Kissen, Ralph; Winge, Per; Tran, Diem Hong Thi; Jørstad, Tommy Stokmo; Størseth, Trond; Christensen, Tone; Bones, Atle M.. (2010) Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome. BMC Genomics. volum 11.
    Academic article

2009

  • Kissen, Ralph; Bones, Atle M.. (2009) Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana. Journal of Biological Chemistry. volum 284 (18).
    Academic article
  • Kissen, Ralph; Pope, Tom W; Grant, Murray; Pickett, John A; Rossiter, John T; Powell, Glen. (2009) Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae. Journal of Chemical Ecology. volum 35 (8).
    Academic article
  • Kissen, Ralph; Rossiter, JT; Bones, Atle M.. (2009) The “mustard oil bomb”: not so easy to assemble?! Localization, expression and distribution of components of the myrosinase enzyme system. Phytochemistry Reviews. volum 8 (1).
    Academic article

2008

  • Kissen, Ralph; Bones, Atle M.. (2008) Characterization of novel nitrile specifier proteins (NSPs) involved in glucosinolate hydrolysis in Arabidopsis thaliana. Physiologia Plantarum : An International Journal for Plant Biology. volum 133 (3).
    Summary/Abstract

Scientific articles

  • Ahuja, Ishita; Kissen, Ralph; Hoang, Linh; Sporsheim, Bjørnar; Halle, Kari Krizak; Wolff, Silje Aase; Ahmad, Samina Jam Nazeer; Ahmad, Jam Nazeer; Bones, Atle M.. (2021) The imaging of guard cells of thioglucosidase (tgg) mutants of Arabidopsis further links plant chemical defence systems with physical defence barriers. Cells. volum 10 (2).
    Academic article
  • Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn; Bones, Atle M.. (2020) Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker. Biotechnology Reports. volum 28.
    Academic article
  • Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn; Bones, Atle M.. (2020) Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker. Biotechnology Reports. volum 28.
    Academic article
  • Ting, Hieng Ming; Cheah, Boon Huat; Chen, Yu-Cheng; Yeh, Pei-Min; Cheng, Piu-Ching; Yeo, Freddy Kuok San; Vie, Ane Kjersti; Rohloff, Jens; Winge, Per; Bones, Atle M.; Kissen, Ralph. (2020) The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses. Frontiers in Plant Science. volum 11.
    Academic article
  • Sharma, Amit Kumar; Mühlroth, Alice; Jouhet, Juliette; Maréchal, Eric; Alipanah, Leila; Kissen, Ralph; Brembu, Tore; Bones, Atle M.; Winge, Per. (2019) The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae. New Phytologist. volum 225 (6).
    Academic article
  • Ruta, Lavinia L; Banu, Melania A.; Neagoe, Aurora D; Kissen, Ralph; Bones, Atle M.; Farcasanu, Ileana C.. (2018) Accumulation of (Ag(I) by Saccharomyces cereviseae cells expressing plant metallothioneins. Cells. volum 7 (12).
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2018) Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate. Scientific Reports. volum 8 (1).
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2018) Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases in Arabidopsis thaliana. Frontiers in Plant Science. volum 9.
    Academic article
  • Ruta, Lavinia Liliana; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora Daniela; Petrescu, Andrei José; Bones, Atle M.; Farcasanu, Ileana Cornelia. (2017) Heavy Metal Accumulation by Saccharomyces cerevisiae Cells Armed with Metal Binding Hexapeptides Targeted to the Inner Face of the Plasma Membrane. Applied Microbiology and Biotechnology. volum 101 (14).
    Academic article
  • Ruta, Lavinia Liliana; Lin, Ya-Fen; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora Daniela; Ghenea, Simona; Bones, Atle M.; Farcasanu, Ileana Cornelia. (2017) Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation. PLOS ONE. volum 12 (5).
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2017) Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on their Side Chain Structure. International Journal of Molecular Sciences. volum 18 (11).
    Academic article
  • Kissen, Ralph; Eberl, Franziska; Winge, Per; Uleberg, Eivind; Martinussen, Inger; Bones, Atle M.. (2016) Effect of growth temperature on glucosinolate profiles in Arabidopsis thaliana accessions. Phytochemistry. volum 130.
    Academic article
  • Kissen, Ralph; Øverby, Anders; Winge, Per; Bones, Atle M.. (2016) Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana. BMC Genomics. volum 17.
    Academic article
  • Kissen, Ralph; Hyldbakk, Elisabeth; Wang, Chia-Wei Vivian; Sørmo, Christopher; Rossiter, John T.; Bones, Atle M.. (2012) Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana. Plant Molecular Biology. volum 78 (4-5).
    Academic article
  • Kong, Xiang Yi; Kissen, Ralph; Bones, Atle M.. (2012) Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: Dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions. Phytochemistry. volum 84.
    Academic article
  • Kissen, Ralph; Winge, Per; Tran, Diem Hong Thi; Jørstad, Tommy Stokmo; Størseth, Trond; Christensen, Tone; Bones, Atle M.. (2010) Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome. BMC Genomics. volum 11.
    Academic article
  • Kissen, Ralph; Bones, Atle M.. (2009) Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana. Journal of Biological Chemistry. volum 284 (18).
    Academic article
  • Kissen, Ralph; Pope, Tom W; Grant, Murray; Pickett, John A; Rossiter, John T; Powell, Glen. (2009) Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae. Journal of Chemical Ecology. volum 35 (8).
    Academic article
  • Kissen, Ralph; Rossiter, JT; Bones, Atle M.. (2009) The “mustard oil bomb”: not so easy to assemble?! Localization, expression and distribution of components of the myrosinase enzyme system. Phytochemistry Reviews. volum 8 (1).
    Academic article

Journal publications

  • Bones, Atle M.; Hara, M; Rossiter, JT; Kissen, Ralph. (2015) Physiology and cellular mechanisms of isothiocyanates and other glucosinolate degradation products in plants. Frontiers in Plant Science.
    Editorial
  • Ahuja, Ishita; Kissen, Ralph; Bones, Atle M.. (2012) Phytoalexins in defense against pathogens. Trends in Plant Science. volum 17 (2).
    Scientific translation/review
  • Kissen, Ralph; Bones, Atle M.. (2008) Characterization of novel nitrile specifier proteins (NSPs) involved in glucosinolate hydrolysis in Arabidopsis thaliana. Physiologia Plantarum : An International Journal for Plant Biology. volum 133 (3).
    Summary/Abstract
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