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  1. Employees

Språkvelger

Norsk

Ralph Kissen

Download press photo
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Ralph Kissen

overingeniør
Department of Biology

ralph.kissen@ntnu.no
+4773596191 DU1-181 Realfagbygget Gløshaugen, Trondheim
ResearchGate
About Publications

About

 

Tasks

  • research support for the Cell and Molecular Biology section
  • technical/administrative tasks (lab responsibility, operation of GMO-lab and analytical equipment, bookitlab, LN2)
  • contribute to teaching in cell and molecular biology
  • train master students on instruments and in techniques

 

Skills

  • extraction and analysis of DNA, RNA and proteins
  • extraction of metabolites and analysis by GC-MS and HPLC
  • expression of recombinant proteins in Escherichia coli, purification and functional characterization (immunoassay, enzyme assay)
  • work with plants:
    • grow in vitro (medium, hydroponics) and on soil
    • transform with Agrobacterium tumefaciens
    • genome editing (CRISPR/Cas9)
    • generate, screen and characterize mutants
    • assays with biotic and abiotic stress factors
  • work with diatoms (Phaeodactylum tricornutum): 
    • grow and maintain kultures
    • transform by biolistics and conjugation
    • genome editing (CRISPR/Cas9)
    • screen og characterize mutant lines

 

Publications

  • Chronological
  • By category
  • All publications registered in NVA

2021

  • Ahuja, Ishita; Kissen, Ralph; Hoang, Linh; Sporsheim, Bjørnar; Halle, Kari Krizak; Wolff, Silje Aase. (2021) The imaging of guard cells of thioglucosidase (tgg) mutants of Arabidopsis further links plant chemical defence systems with physical defence barriers. Cells
    Academic article

2020

  • Ting, Hieng Ming; Cheah, Boon Huat; Chen, Yu-Cheng; Yeh, Pei-Min; Cheng, Piu-Ching; Yeo, Freddy Kuok San. (2020) The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses. Frontiers in Plant Science
    Academic article
  • Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn. (2020) Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker. Biotechnology Reports
    Academic article
  • Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn. (2020) Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker. Biotechnology Reports
    Academic article

2019

  • Sharma, Amit Kumar; Mühlroth, Alice; Jouhet, Juliette; Maréchal, Eric; Alipanah, Leila; Kissen, Ralph. (2019) The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae. New Phytologist
    Academic article

2018

  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2018) Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases in Arabidopsis thaliana. Frontiers in Plant Science
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2018) Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate. Scientific Reports
    Academic article
  • Ruta, Lavinia L; Banu, Melania A.; Neagoe, Aurora D; Kissen, Ralph; Bones, Atle M.; Farcasanu, Ileana C.. (2018) Accumulation of (Ag(I) by Saccharomyces cereviseae cells expressing plant metallothioneins. Cells
    Academic article

2017

  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2017) Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on their Side Chain Structure. International Journal of Molecular Sciences
    Academic article
  • Ruta, Lavinia Liliana; Lin, Ya-Fen; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora Daniela; Ghenea, Simona. (2017) Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation. PLOS ONE
    Academic article
  • Ruta, Lavinia Liliana; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora Daniela; Petrescu, Andrei José; Bones, Atle M.. (2017) Heavy Metal Accumulation by Saccharomyces cerevisiae Cells Armed with Metal Binding Hexapeptides Targeted to the Inner Face of the Plasma Membrane. Applied Microbiology and Biotechnology
    Academic article

2016

  • Kissen, Ralph; Eberl, Franziska; Winge, Per; Uleberg, Eivind; Martinussen, Inger; Bones, Atle M.. (2016) Effect of growth temperature on glucosinolate profiles in Arabidopsis thaliana accessions. Phytochemistry
    Academic article
  • Kissen, Ralph; Øverby, Anders; Winge, Per; Bones, Atle M.. (2016) Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana. BMC Genomics
    Academic article

2015

  • Bones, Atle M.; Hara, M; Rossiter, JT; Kissen, Ralph. (2015) Physiology and cellular mechanisms of isothiocyanates and other glucosinolate degradation products in plants. Frontiers in Plant Science
    Editorial

2012

  • Kong, Xiang Yi; Kissen, Ralph; Bones, Atle M.. (2012) Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: Dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions. Phytochemistry
    Academic article
  • Ahuja, Ishita; Kissen, Ralph; Bones, Atle M.. (2012) Phytoalexins in defense against pathogens. Trends in Plant Science
    Academic literature review
  • Kissen, Ralph; Hyldbakk, Elisabeth; Wang, Chia-Wei Vivian; Sørmo, Christopher; Rossiter, John T.; Bones, Atle M.. (2012) Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana. Plant Molecular Biology
    Academic article

2010

  • Kissen, Ralph; Winge, Per; Tran, Diem Hong Thi; Jørstad, Tommy Stokmo; Størseth, Trond; Christensen, Tone. (2010) Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome. BMC Genomics
    Academic article

2009

  • Kissen, Ralph; Rossiter, JT; Bones, Atle M.. (2009) The “mustard oil bomb”: not so easy to assemble?! Localization, expression and distribution of components of the myrosinase enzyme system. Phytochemistry Reviews
    Academic article
  • Kissen, Ralph; Bones, Atle M.. (2009) Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana. Journal of Biological Chemistry
    Academic article
  • Kissen, Ralph; Pope, Tom W; Grant, Murray; Pickett, John A; Rossiter, John T; Powell, Glen. (2009) Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae. Journal of Chemical Ecology
    Academic article

2008

  • Kissen, Ralph; Bones, Atle M.. (2008) Characterization of novel nitrile specifier proteins (NSPs) involved in glucosinolate hydrolysis in Arabidopsis thaliana. Physiologia Plantarum : An International Journal for Plant Biology
    Abstract

Journal publications

  • Ahuja, Ishita; Kissen, Ralph; Hoang, Linh; Sporsheim, Bjørnar; Halle, Kari Krizak; Wolff, Silje Aase. (2021) The imaging of guard cells of thioglucosidase (tgg) mutants of Arabidopsis further links plant chemical defence systems with physical defence barriers. Cells
    Academic article
  • Ting, Hieng Ming; Cheah, Boon Huat; Chen, Yu-Cheng; Yeh, Pei-Min; Cheng, Piu-Ching; Yeo, Freddy Kuok San. (2020) The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses. Frontiers in Plant Science
    Academic article
  • Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn. (2020) Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker. Biotechnology Reports
    Academic article
  • Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn. (2020) Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker. Biotechnology Reports
    Academic article
  • Sharma, Amit Kumar; Mühlroth, Alice; Jouhet, Juliette; Maréchal, Eric; Alipanah, Leila; Kissen, Ralph. (2019) The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae. New Phytologist
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2018) Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases in Arabidopsis thaliana. Frontiers in Plant Science
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2018) Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate. Scientific Reports
    Academic article
  • Ruta, Lavinia L; Banu, Melania A.; Neagoe, Aurora D; Kissen, Ralph; Bones, Atle M.; Farcasanu, Ileana C.. (2018) Accumulation of (Ag(I) by Saccharomyces cereviseae cells expressing plant metallothioneins. Cells
    Academic article
  • Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph. (2017) Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on their Side Chain Structure. International Journal of Molecular Sciences
    Academic article
  • Ruta, Lavinia Liliana; Lin, Ya-Fen; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora Daniela; Ghenea, Simona. (2017) Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation. PLOS ONE
    Academic article
  • Ruta, Lavinia Liliana; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora Daniela; Petrescu, Andrei José; Bones, Atle M.. (2017) Heavy Metal Accumulation by Saccharomyces cerevisiae Cells Armed with Metal Binding Hexapeptides Targeted to the Inner Face of the Plasma Membrane. Applied Microbiology and Biotechnology
    Academic article
  • Kissen, Ralph; Eberl, Franziska; Winge, Per; Uleberg, Eivind; Martinussen, Inger; Bones, Atle M.. (2016) Effect of growth temperature on glucosinolate profiles in Arabidopsis thaliana accessions. Phytochemistry
    Academic article
  • Kissen, Ralph; Øverby, Anders; Winge, Per; Bones, Atle M.. (2016) Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana. BMC Genomics
    Academic article
  • Bones, Atle M.; Hara, M; Rossiter, JT; Kissen, Ralph. (2015) Physiology and cellular mechanisms of isothiocyanates and other glucosinolate degradation products in plants. Frontiers in Plant Science
    Editorial
  • Kong, Xiang Yi; Kissen, Ralph; Bones, Atle M.. (2012) Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: Dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions. Phytochemistry
    Academic article
  • Ahuja, Ishita; Kissen, Ralph; Bones, Atle M.. (2012) Phytoalexins in defense against pathogens. Trends in Plant Science
    Academic literature review
  • Kissen, Ralph; Hyldbakk, Elisabeth; Wang, Chia-Wei Vivian; Sørmo, Christopher; Rossiter, John T.; Bones, Atle M.. (2012) Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana. Plant Molecular Biology
    Academic article
  • Kissen, Ralph; Winge, Per; Tran, Diem Hong Thi; Jørstad, Tommy Stokmo; Størseth, Trond; Christensen, Tone. (2010) Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome. BMC Genomics
    Academic article
  • Kissen, Ralph; Rossiter, JT; Bones, Atle M.. (2009) The “mustard oil bomb”: not so easy to assemble?! Localization, expression and distribution of components of the myrosinase enzyme system. Phytochemistry Reviews
    Academic article
  • Kissen, Ralph; Bones, Atle M.. (2009) Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana. Journal of Biological Chemistry
    Academic article
  • Kissen, Ralph; Pope, Tom W; Grant, Murray; Pickett, John A; Rossiter, John T; Powell, Glen. (2009) Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae. Journal of Chemical Ecology
    Academic article
  • Kissen, Ralph; Bones, Atle M.. (2008) Characterization of novel nitrile specifier proteins (NSPs) involved in glucosinolate hydrolysis in Arabidopsis thaliana. Physiologia Plantarum : An International Journal for Plant Biology
    Abstract

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