Background and activities
Biomineralization, the formation of complex inorganic structures by organisms, is a widely distributed process in nature. One of the most spectacular examples of biomineralization is the diatom cell wall (frustule), which is a three-dimensional silica structure with intricate, species-specific patterns ranging from nano- to micrometer scale. While a number of proteins have been identified that take part in the deposition and patterning of silica within a specialized compartment (the silica deposition vesicle, SDV), the process as a whole is poorly understood. There is extensive interaction between the inside of the SDV and cytosolic factors such as the cytoskeleton, but the mechanisms of these interactions are unknown.
We have identified a gene family restricted to diatoms that encodes predicted transmembrane proteins with a yet uncharacterised domain. Several lines of evidence suggest that members of this protein family, termed Silicanins, are localized to the SDV membrane, and that they are involved in frustule biosynthesis. In this project, we aim to investigate the role of Silicanins in cell wall biomineralization, using state-of-the-art molecular, biochemical and imaging techniques.
Scientific, academic and artistic work
Displaying a selection of activities. See all publications in the database
- (2020) The Seminavis robusta genome provides insights into the evolutionary adaptations of benthic diatoms. Nature Communications. vol. 11 (1).
- (2019) PAMP-INDUCED SECRETED PEPTIDE 3 modulates immunity in Arabidopsis. Journal of Experimental Botany. vol. 71 (3).
- (2019) The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae. New Phytologist. vol. 225 (6).
- (2018) Molecular adaptations to phosphorus deprivation and comparison with nitrogen deprivation responses in the diatom Phaeodactylum tricornutum. PLOS ONE. vol. 13 (2).
- (2017) Dynamic responses to silicon in Thalasiossira pseudonana - Identification, characterisation and classification of signature genes and their corresponding protein motifs. Scientific Reports. vol. 7 (1).
- (2017) The effects of phosphorus limitation on carbon metabolism in diatoms. Philosophical Transactions of the Royal Society of London. Biological Sciences. vol. 372 (1728).
- (2017) The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana. Journal of Experimental Botany. vol. 68 (13).
- (2016) A sex-inducing pheromone triggers cell cycle arrest and mate attraction in the diatom Seminavis robusta. Scientific Reports. vol. 6.
- (2015) Whole cell response to nitrogen depletion in the diatom Phaeodactylum tricornutum. Journal of Experimental Botany. vol. 66 (20).
- (2015) The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: Phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide. Journal of Experimental Botany. vol. 66 (17).
- (2014) The chloroplast genome of the diatom Seminavis robusta: New features introduced through multiple mechanisms of horizontal gene transfer. Marine Genomics. vol. 16 (1).
- (2014) System responses to equal doses of photosynthetically usable radiation of blue, green, and red light in the marine diatom Phaeodactylum tricornutum. PLOS ONE. vol. 9 (12).
- (2013) Gene regulation of carbon fixation, storage, and utilization in the diatom phaeodactylum tricornutum acclimated to light/dark cycles. Plant Physiology. vol. 161 (2).
- (2013) NEVERSHED and INFLORESCENCE DEFICIENT IN ABSCISSION are differentially required for cell expansion and cell separation during floral organ abscission in Arabidopsis thaliana. Journal of Experimental Botany. vol. 64 (17).
- (2013) Molecular and photosynthetic responses to prolonged darkness and subsequent acclimation to re-illumination in the diatom Phaeodactylum tricornutum. PLOS ONE. vol. 8 (3).
- (2011) Genome-wide profiling of responses to cadmium in the diatom Phaeodactylum tricornutum. Environmental Science and Technology. vol. 45 (18).
- (2011) Arabidopsis thaliana MIRO1 and MIRO2 GTPases Are Unequally Redundant in Pollen Tube Growth and Fusion of Polar Nuclei during Female Gametogenesis. PLOS ONE. vol. 6 (4).
- (2009) Plant peptides in signalling: looking for new partners. Trends in Plant Science. vol. 14 (5).
- (2009) An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum. PLOS ONE. vol. 4 (11).
- (2006) A RHOse by any other name: a comparative analysis of animal and plant Rho GTPases. Cell Research. vol. 16 (5).
- (2006) The crystal structure of Arabidopsis thaliana RAC7/ROP9: The first RAS superfamily GTPase from the plant kingdom. Phytochemistry. vol. 67 (21).
- (2005) Catching the WAVEs of plant actin regulation. Journal of Plant Growth Regulation. vol. 24.
- (2005) The small GTPase AtRAC2/ROP7 is specifically expressed during late stages of xylem differentiation in Arabidopsis. Journal of Experimental Botany. vol. 56 (419).
- (2004) NAPP and PIRP encode subunits of a putative WAVE regulatory protein complex involved in plant cell morphogenesis. The Plant Cell. vol. 16 (9).
- (2000) Genetic structure and evolution of Rac-GTPases in Arabidopsis thaliana. Genetics. vol. 156 (4).
- (1997) Cloning and characterization of rac-like cDNAs from Arabidopsis thaliana. Plant Molecular Biology. vol. 35.