Vivien Jessica Klein
About
I have a background in biotechnology and bioprocess development. In my PhD thesis research at the Brautaset Lab at the Department of Biotechnology and Food science I focused on metabolic engineering of industrially relevant strains for the production of riboflavin from alternative/sustainable feedstocks like methanol and brown seaweed biomass. The use of methanol as sustainable feedstock was investigated with regards to future establishment of synthetic methylotrophy in B. subtilis that is routinely used in the biotechnological industry. Therefore, I studied, among others, formaldehyde assimilation and dissimilation pathways in bacteria. At the same time I investigated achieving methanol-based riboflavin production with the help of the systems biology understanding of the C1 metabolism of native methylotrophs such as B. methanolicus MGA3. Furthermore, I gathered experience with microbial fermentation and HPLC.
Curently I work as a lab engineer at the Department of Biology. I am responsible for the maintenance of laboratories in D1 and DU1 and assisst with preparations for the lab courses BI1001, BI1007 and BI2015. Furthermore, I support the environmental toxicology group (Martin Wagner) with ordering equipment and chemicals, and conduction of experiments.
Publications
2023
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Klein, Vivien Jessica;
Fernandes de Brito, Luciana;
Perez Garcia, Fernando;
Brautaset, Trygve;
Irla, Marta Katarzyna.
(2023)
Metabolic engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol.
Microbial Biotechnology
Academic article
2022
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Perez-Garcia, Fernando;
Klein, Vivien Jessica;
Fernandes de Brito, Luciana;
Brautaset, Trygve.
(2022)
From Brown Seaweed to a Sustainable Microbial Feedstock for the Production of Riboflavin.
Frontiers in Bioengineering and Biotechnology
Academic article
-
Klein, Vivien Jessica;
Irla, Marta Katarzyna;
Gil Lopez, Marina;
Brautaset, Trygve;
Fernandes de Brito, Luciana.
(2022)
Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.
Microorganisms
Academic literature review
Journal publications
-
Klein, Vivien Jessica;
Fernandes de Brito, Luciana;
Perez Garcia, Fernando;
Brautaset, Trygve;
Irla, Marta Katarzyna.
(2023)
Metabolic engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol.
Microbial Biotechnology
Academic article
-
Perez-Garcia, Fernando;
Klein, Vivien Jessica;
Fernandes de Brito, Luciana;
Brautaset, Trygve.
(2022)
From Brown Seaweed to a Sustainable Microbial Feedstock for the Production of Riboflavin.
Frontiers in Bioengineering and Biotechnology
Academic article
-
Klein, Vivien Jessica;
Irla, Marta Katarzyna;
Gil Lopez, Marina;
Brautaset, Trygve;
Fernandes de Brito, Luciana.
(2022)
Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.
Microorganisms
Academic literature review
Media
2019
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LectureKlein, Vivien Jessica. (2019) Metabolic engineering of industrially relevant Bacillus species for the production of riboflavin from methanol . IBT IBT's weekly Department lunch , IBT's lunch room, NV Faculty, NTNU, Gløshaugen 2019-12-04 -
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LectureKlein, Vivien Jessica. (2019) Metabolic engineering of B. methanolicus for production of riboflavin and its derivatives. IBT IBT's weekly Department lunch , IBT's lunch room, NV Faculty, NTNU, Gløshaugen 2019-03-27 -