Jens Rohloff

Researcher, Adj.Assoc.Prof. Department of Biology Faculty of Natural Sciences

+47 73596093 +47 97608994
Realfagbygget, DU1-172, Gløshaugen, Høgskoleringen 5

Background and activities

S h o r t   B i o
Education : :
2003 - PhD at Dep. Biology, NTNU, Trondheim
“Cultivation of herbs and medicinal plants in Norway - Essential oil production and quality control”
1996 - Cand. scient. in Botany, Plant Physiology, Dep. Botany, NTNU, Trondheim
"Yield and quality in raspberry (Rubus idaeus L.) leaf production
1991 - in Plant Production, Dep. Plant Nutr & Soil Sci, Christian-Albrecht University Kiel, Germany
Nitrate mobility in soils of Schleswig-Holstein is affected by agricultural use
Experience : :
2014- Adj. Assoc. Prof. / Plant Physiology
2005- Researcher at Dep. Biology, NTNU, Trondheim
2003-2004    Post-doc at Allforsk & Dep. Biology, NTNU, Trondheim
1997-2002 Research Assistant/ Researcher at Allforsk, NTNU, Trondheim
Publications : :
53 articles in international journals, 6 book chapters, 6 articles in national journals, 34 reports, 48 scientific lectures and 44 posters
S c i e n t i f i c   P r o f i l e
Plants impart life and represent an absolute necessity for the survival of mankind on earth. As autotrophic organisms, plants supply our atmosphere with essential oxygen, and furthermore, constitute the basis for the production of food and feed, fire wood and biofuel, medicinal substances, and raw materials for technical and industrial processing. Throughout evolution, plants have developed innumerable biochemical structures and compounds – to live on and literally, to die of.
Based on my agricultural and plant biological background, I am particularly interested in the overwhelming diversity found in the plant kingdom with research focus on food, feed, and bioactive compounds. Besides target compound analysis, i.e. single compounds or compound groups, I work also with metabolite profiling with regard to stress (abiotic and biotic factors), physiological processes, phytochemical diversity, food quality and nutritional value. In the same way as transcriptional and protein profiling, metabolite profiling (GC/MS, UPLC-TOF/MS) can yield extensive chemical information of hundreds of compounds in one analytical run, thereby providing substantial information to describe an organism's actual state, the regulation of metabolic networks, physiological functions and processes.
FoodMaP - Food Metabolites and Phytochemicals - The research group was earlier located at the Plant Biocentre and today includes one research engineer and several master students. Project activities are based on the application of metabolomics methods with focus on agriculture-, food- and health-related questions. At the department level, the group has strong co-operation with the Cell, Molecular Biology and Genomics Group (CMBG), and Dep. Biotechnology (Food Chemistry). Since 2010, 8 students finished their master projects under my supervision/ co-supervision with theses linked to FoodMaP.
Besides research activities, I have been involved for years in teaching at the Dep. Biology in the following courses: BI1001 Cell and Molecular Biology, BI2012 Cell Biology, BI3013 Experimental Cell and Molecular Biology, and BI1004 Physiology and BI3020 Advanced Physiology from 2014.
On-going and Recently Finalized Projects
EUROMA - Benchmarking of master programmes across European countries (NO: NOKUT, IBI/NTNU, UiB, UiT; The Netherlands and Sweden), 2016-17 (Info)
Improved Microbial Quality and Safety of Fish (SAFEFISHDISH), 2015-17 (ERA-Net COFASP)
Sami perfume (VRI Finnmark), 2016
Quality of meat from lamb and sheep (Guest researcher V. Grabez, PhD-student NMBU), 2014
Effect of plant extracts on decomposing microbial biofilm in drinking water systems (Guest researcher project: Dr. Bazargani), 2013+14
Biological-active components in raw materials and effects in fish: how to produce aquafeeds without fish meal, 2012-15 (NFR no. 217485)
Eco-values as product quality attributes in manufacturing of agricultural food ingredients, 2011-15 (NFR no. 207761)
Study of metabolic cold acclimation/ deacclimation in Fragaria x ananassa using UPLC/TOF-MS2 and GC/TOF-MS profiling: Genome Center, UC Davis, CA, 2011
Improving winter survival and quality of Norwegian cultivated strawberry, 2010-14 (NFR no. 199554)
Sources of plant protein for fish feed: Understanding and controlling antinutritional factors, 2008-11 (NFR no. 187294)
Potential of European blueberry (Vaccinium myrtillus) for cultivation and industrial exploitation in Norway, 2008-11 (NFR no. 184797)
Development and application of a customized Fragaria microarray, 2010
Identification of molecular markerks associated with winter survival in the cultivated strawberry, 2007-10 (NFR no. 179466)
Essential oil content and composition in Norwegian herbs and medicinal plants, 1998-  (NIBIO)
Other Research Activities
Glucosinolate-myrosinase system and plant defence in Cruciferae plant species
Natural compounds and biological activity in indigenous plants from Ethiopia
Science boards, committees & editorial work
2017-  FRONTIERS IN PLANT SCIENCE Associate editor, section Plant Metabolism and Chemodiversity
2014-2018 Member of the Norwegian Scientific Committee for Food Safety (VKM) – Scientific Panel on Food Additives
→   2013– LOSAM Works council at NT Faculty – union representative (dep.) for Forskerforbundet (The Norwegian Association of Researchers NAR)
2012–2015 COST Action FA 1006 – PlantEngine: Plant Metabolic Engineering for High Value Products, Working Group 1 – "Status quo and Road Map"
2012– FRONTIERS IN PLANT SCIENCE/ Plant Metabolism and Chemodiversity - review editor, guest editor (2014/15)
2011-2014 Safety representative (deputy) at Dep Biology, NTNU
2002– Referee for several international scientific journals within biology, agriculture, food and chemistry (e.g. J.Agric. Food Chem., Phytochemistry, Chromatographia, BMC Biol., etc)
Master and PhD Projects (2009-2016)
Anku, K.E. (MSc) 2016-17 Effect of heat stress on photosynthesis and metabolism in maize (IBI) [on-going]
Begangancha, E.H. (MSc) 2016-17 Testing a home cultivation system for production of herbs and greens: Effects of light on food quality (IBI) [on-going]
Bahita, T.L. (MSc) 2015-17 Essential oil and biodiversity study of Securidaca longependiculata (IBI) [on-going]
Hjermstad-Sollerud, H. (MSc) 2014-16 Physiological and biochemical responses of the arctic plant Papaver dahlianum upon heat waves (IBI)
Negard, A. (MSc) 2013-15 Stress responses induced by temperature and light conditions in Brassica napus (IBI)
Nguyen, T. (MSc) 2013-15 Influence of feed ingredients on lipid metabolism and quality parameters of cow's milk (IBI)
Alipanah, L. (PhD) 2011-15 Integrated response of the diatoms Phaeodactylum tricornutum and Seminavis robusta to nutrient deprivation (IBI)
Skrutvold, E. (MSc) 2014 Effects of storage and heat treatment on metabolism and quality of fresh tomato and potato (IBI)
Dhuli, P. (MSc) 2011 Metabolite changes in conifer buds and needles during bud break - Norway spruce (Picea abies) and European silver fir (Abies alba) (IBI)
Ytterdal, I.B. (MSc) 2011 Genetic and phytochemical diversity in bilberry (Vaccinium myrtillus) from a limited geographical area (IBI & NIBIO)
Dahlø, E.S. (MSc) 2011 Variation in chemical composition and genetic differentiation among bilberry (Vaccinium myrtillus L.) populations on a latitudinal gradient (IBI & NIBIO)
Eidem, P. (MSc) 2010 Cold acclimation in strawberry (Fragaria sp.) genotypes: Description of metabolic plasticity (IBI & NIBIO)
Eyob Ferenje, S. (PhD) 2009 Potential of korarima (Aframomum corrorima (Braun) P.C.M. Jansen) as a crop plant in Southern Ethiopia (NMBU/ IPM, Debub Univ./ Ethiopia & NTNU/ IBI)
Most Recent Publications (2010-2016)
Ahuja, I., De Vos R.C.H.,  Rohloff, J., Stoopen, G., Halle, K.K., Nazeer Ahmad, S.J., Hoang, L., Hall, R.D., and Bones, A.M. 2016 Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle. Sci. Rep. 6:38990 pdf
Prestegard, S.K., Erga, S.R., Steinrücken, P., Mjøs, S.A., Knutsen, G., and Rohloff, J. 2016 Specific metabolites in a Phaeodactylum tricornutum strain isolated from Western Norwegian fjord water. Mar. Drugs 14(1): 9 pdf
Bazargani, M.M., and Rohloff, J. 2016 Antibiofilm activity of essential oils and plant extracts against Staphylococcus aureus and Escherichia coli biofilms. Food Control 61:156-164 URL
Rohloff, J., Steffensen, I.-L., Bruzell, E., Granum, B., Hetland, R.B., Husøy, T., and Wicklund, T. 2016 Risk assessment of "other substances" – Piperine. Opinion of the Panel on Food Additives, Flavourings, Processing Aids, Materials in Contact with Food and Cosmetics of the Norwegian Scientific Committee for Food Safety, VKM Report 2016:31, Oslo, Norway. pdf
Rohloff, J., Steffensen, I.-L., Bruzell, E., Granum, B., Hetland, R.B., Husøy, T., and Wicklund, T. 2015 Risk assessment of "other substances" – Inositol. Opinion of the Panel on Food Additives, Flavourings, Processing Aids, Materials in Contact with Food and Cosmetics of the Norwegian Scientific Committee for Food Safety, VKM Report 2015:23, Oslo, Norway. pdf
Rohloff, J., Uleberg, E., Nes, A., Krogstad, A., Nestby, R., and Martinussen, I. 2015 Nutritional composition of bilberries (Vaccinium myrtillus L.) from forest fields in Norway - Effects of geographic origin, climate, fertilization, and soil properties. J. Appl. Bot. Food Qual. 88: 274-287. pdf
Koehler, G., Rohloff, J., Wilson, R.C., Kopka, J., Erban, A., Winge, P., Bones, A.M., Davik, J., Alsheikh, M., and Randall, S.K. 2015 Integrative “omic” analysis reveals distinctive cold responses in leaves and roots of strawberry, Fragaria × ananassa ‘Korona’. Front. Plant Sci. 6: art. 826 pdf
Alipanah, L., Rohloff, J., Winge, P., Bones, A.M., and Brembu, T. 2015 Whole cell response to nitrogen depletion in the diatom Phaeodactylum tricornutum. J. Exp. Bot. 66:6281-6296. pdf
Rohloff, J. 2015 Effekter av tørking og termisk behandling på næringsverdier og kvalitet i råvarer og prosesserte matvarer. In Norske matvarer - Verdier, muligheter og utfordringer. Ed. Iversen, N.M. Fagbokforlaget, Bergen, Norway; pp. 79-107. [Norwegian]
Rohloff, J. 2015 Analysis of polyphenolic and cyclic compounds in plants using derivatization techniques in combination with GC-MS-based metabolite profiling. Molecules 20(2): 3431-3462. pdf
Ahuja, I., Van Dam, N.M., Winge, P., Trælnes, M., Heydarova, A., Rohloff, J., Langaas, M., and Bones, A.M. 2015 Plant defence responses in oilseed rape MINELESS plants after attack by the cabbage moth Mamestra brassicae. J. Exp. Bot. 66: 579-592. pdf
Dhuli, P., Rohloff, J., and Strimbeck, R. 2014 Metabolite changes in conifer buds and needles during forced bud break in Norway spruce (Picea abies) and European silver fir (Abies alba). Front. Plant Sci. 5:706. URL
VKM 2014 Risk assessments of aspartame, acesulfame K, sucralose and benzoic acid from soft drinks, “saft”, nectar and flavoured water. Opinion of the Panel on Food Additives, Norwegian Scientific Committee for Food Safety (VKM), ISBN: 978-82-8259-151-5, Oslo, Norway, pp.61. pdf
Iversen, T.-H., Myskja, B.K., and Rohloff, J. 2014 Norsk melk danker ut hvite EU dråper. Bergens Tidende Søndag 9. nov 2014, 147(nr. 301): 16-17. [Norwegian] URL
Rohloff, J., Eidem, P., Davik, J., and Alsheikh, M. 2014 Metabolic cold acclimation of ‘Polka' and ‘Honeoye' strawberries under natural field conditions. Acta Hort. 1049: 463-466. URL
Iversen, T.-H., Rohloff, J., Christiansen, A. 2013 Genmodifiserte organismer og norske matvarer. Matindustrien 7/8: 36-38. [Norwegian]
Uleberg, E., Rohloff, J., Jaakola, L., Trost, K., Junttila, O., Häggman, H., and Martinussen, I. 2013 Både miljø og genetikk regulerer innholdet av bioaktive forbindelser i blåbær. Bioforsk FOKUS 8(2): 271-272. [Norwegian] pdf
Iversen, T.-H., Rohloff, J., Christiansen, A. 2013 Plantevernmidler i norske matvarer? Matindustrien 6: 34-36. [Norwegian]
Davik, J., Koehler, G., From, B., Torp, T., Rohloff, J., Eidem, P., Wilson, R.C., Sønsteby, A., Randall, S.K., Alsheikh, M. 2013 Dehydrin, alcohol dehydrogenase, and central metabolite levels are associated with cold tolerance in diploid strawberry (Fragaria spp.). Planta 237: 265-277. URL
Rohloff, J., Hymete, A., and Tariku, Y. 2013 Plant-derived natural products for the treatment of leishmaniasis. In Studies in Natural Products Chemistry – Bioactive Natural Products Vol. 39, Ed. A.-U. Rahman. Elsevier, Amsterdam, The Netherlands, pp. 381-429.
Uleberg, E., Rohloff, J., Jaakola, L., Trôst, K., Junttila, O., Häggman, H., and Martinussen, I. 2012 Effects of temperature and photoperiod on yield and chemical composition of Northern and Southern clones of bilberry (Vaccinium myrtillus L.). J. Agric. Food Chem. 60: 10406-10414. URL
Koehler, G., Wilson, R.C., Goodpaster, J.V., Sønsteby, A., Lai, X., Witzmann, F.A., You, J.-S., Rohloff, J., Randall, S.K. and Alsheikh, M. 2012 Proteomic study of low temperature responses in strawberry cultivars (Fragaria x ananassa Duchesne) that differ in cold tolerance. Plant Physiol. 159(4): 1787-1805. pdf
Rohloff, J., Kopka, J., Erban, A., Winge, P., Wilson, R.C., Bones, A.M., Davik, J., Randall, S.K. and Alsheikh, M. 2012 Metabolite profiling reveals novel multi-level cold responses in the diploid model Fragaria vesca (woodland strawberry). Phytochemistry 77: 99-109. URL
Wolff, S., Karoliussen, I., Rohloff, J., and Strimbeck, R. 2012 Foliar applications of silicon fertilisers inhibit powdery mildew development in greenhouse cucumber. J. Food Agric. Envir. 10(1): 355-359. URL
Borgen, B.H., Ahuja, I., Thangstad, O.P., Honne, B.I., Rohloff, J., Rossiter, J.T., and Bones, A.M. 2012 ‘Myrosin cells' are not a prerequisite for aphid feeding on oilseed rape (Brassica napus); but affect host plant preferences. Plant Biol. 14(6): 894-904. URL
Rohloff, J., Barah, P., and Bones, A.M. 2012 Improving crop productivity and abiotic stress tolerance in cultivated Fragaria. In Improving Crop Productivity in Sustainable Agriculture. Eds. Tuteja, N., Gill, S.S., and Tuteja, R. Wiley-VCH, Weinheim, Germany, pp.451-484 [ISBN 978-3-527-33242-7].
Koehler, G., Randall, S.K., Wilson, R.C., Winge, P., Rohloff, J., and Alsheikh, M. 2012 Molecular cold responses for two strawberry cultivars: Comparison of proteomic and microarray analysis. Acta Hort. 929: 73-79. URL
Chikwati, E.M., Venold, F.F., Penn, M.H., Rohloff, J., Refstie, S., Guttvik, A., Krogdahl, Å., and Hillestad, M. 2012 Interaction of soyasaponins with plant ingredients in diets for Atlantic salmon, Salmo salar L. Br. J. Nutr. 107(11): 1570-1590. URL
Ahuja, I., Borgen, B.H., Hansen, M., Honne, B.I., Müller, C., Rohloff, J., Rossiter, J.T., and Bones, A.M. 2011 Oilseed rape seeds with ablated defence cells of the glucosinolate-myrosinase system - Production and characteristics of double haploid MINELESS plants of Brassica napus L. J. Exp. Bot. 62(14): 4975-4993. pdf
Ahuja, I., Rohloff, J., and Bones, A.M. 2011 Defence mechanisms of Brassicaceae: Implications for plant-insect interactions and potential for integrated pest management. In: Sustainable Agriculture – Volume 2. Eds. Lichtfouse, E., Hamelin, M., Vavarrete, M., and Debaeke, P. Springer, Dordrecht, Heidelberg, London, New York, pp.623-670.
Rohloff, J. 2011 Impact of agricultural and environmental factors on strawberry (Fragaria x ananassa Duch.) aroma – a review. Eur. J. Plant Sci. Biotechnol. 5(Special Issue I): 17-34. pdf
Tariku, Y., Hymete, A., Hailu, A. and Rohloff, J. 2011 In vitro evaluation of antileishmanial activity and toxicity of essential oils of Artemisia absinthium and Echinops kebericho. Chem. Biodiv. 8(4): 614-623. URL
Sissener, N. I. Hemre, G.-I., Lall, S. P., Sagstad, A. Petersen, K., Williams, J., Rohloff, J., and Sanden, M. 2011 Are apparent negative effects of feeding genetically modified MON810 maize to Atlantic salmon, Salmo salar caused by confounding factors? Br. J. Nutr. 106(1): 42-56. URL
Nestby, R., Percival, D., Martinussen, I., Opstad, N., and Rohloff, J. 2011 The European blueberry (Vaccinium myrtillus) and the potential for cultivation. A review. Eur. J. Biotechnol. 5(Special Issue I): 5-16. pdf
Tariku, Y., Hymete, A., Hailu, A. and Rohloff, J. 2010 Phytochemical constituents, in vitro antileishmanial activity and toxicity profile of volatile oil from berries of Croton macrostachyus. Nat. Prod. Comm. 5(6): 975-980.
Hussien, J., Rohloff, J. and Hymete, A. 2010 Volatile constituents and biological activity of the extracts of Pycnostachys abyssinica and Pycnostachys eminii. Pharm. Biol. 48: 1384-1391. URL
Tariku, Y., Hymete, A., Hailu, A. and Rohloff, J. 2010 Essential oil composition, antileishmanial and toxicity study of Artemisia abyssinica and Satureja punctata ssp. punctata from Ethiopia. Chem. Biodiv. 7(4):1009-1018. URL
Ahuja, I., Rohloff, J., and Bones, A.M. 2010 Defence mechanisms of Brassicaceae: Implications for plant-insect interactions and potential for integrated pest management. A review. Agron. Sustain. Dev. 30:311-348. URL
Hellum, B.H., Tosse, A., Høybakk, K., Thomsen, M.W., Rohloff, J., and Nilsen, O.G. 2010 Potent in vitro inhibition of CYP3A4 and P-glycoprotein by Rhodiola rosea. Planta Med. 76: 331-338. URL
(updated March 12th 2017)