Background and activities
Human genome is exposed to variety of external and internal factors which can disrupt its stability and integrity. [Papamichos-Chronakis, et al. 2013]. Therefore, DNA within each cell has to continuously undergo specific repair mechanisms in order to avoid the critical effects. One of the major pathway is Base Excision Repair (BER) [Yi et al. 2013]. The genes important for the cell survival upon the alkylating agents and the protein complexes involve into damage recognition as well as their role in the DNA repair have not been fully characterized. The further investigation into genomic and proteomic factors responsible for balanced BER seems to be a substantial goal in many cancer and neurodegenerative diseases. The imperative hypothesis of my PhD project is that alkylation stress is responsible for DNA damage, resulting in activation of several repair networks, which disequilibrium might provoke carcinogenesis. The primary aim of the project is to provide prospective results which might contribute to novel diagnostic and new therapy implementation in cancer provoked by alkylation DNA damage.