Background and activities
Stokke leads a biophysics based research activity at the interface between life-science and engineering, with emphasis on physical properties of biological macromolecules, their assemblies, e.g. hydrogels. Mesoscale structure formation and interactions within biopolymers are fundamental phenomena underpinning molecular understanding of biopolymers and their technological exploitation. We study details of biopolymer interactions, association states occurring within polyelectrolyte complexes, biopolymer assemblies and multilayers, polysaccharide gels, and responsive gels as biospecific signal transducers. The activity covers a range of various biological macromolecules and their properties domains as exemplified by information provided in the following links:
Single-molecular pair interactions
Bioresponsive hydrogels as signal transducing materials in biosensors
Structure and properties of beta-glucans
Microfluidics – devices for preparation of hydrogels with controlled size, and separation of exosomes
Amyloid structures studied by AFM and TIRFM
Structure of polysaccharide hydrogels
Nanoscopic characterization of Toll-like receptors
Physics of the enzymatic mode of action
In addition to classical ensemble averaging techniques, application of single-molecule techniques is a distinctive facet of our approach to tackle core issues within these topics. The tools implemented locally for this research include atomic force microscopy, total internal reflection fluorescence microscopy, dynamic force spectroscopy, high resolution interferometry and rheology. These tools are complemented by additional techniques made available to through collaborations with laboratories either locally, or within national and international collaborations. We are also increasing taking advantage of parts of the micro-and nanoscale fabrication facilities provided by NTNU NanoLab, e.g., for custom design of fabricated microfluidic chips. A distintiive feature of the research efforts is also the combination of experimental approaches with numerical/theoretical ones, e.g, finite element modelling of bioresponsive hydrogels, modelling of enzymatic mode of action, and implementation of efficient routines for extraction of quantitative parameters (e.g. force spectroscopy, image analysis).
Scientific, academic and artistic work
A selection of recent journal publications, artistic productions, books, including book and report excerpts. See all publications in the database
- (2018) In vitro single-cell dissection revealing the interior structure of cable bacteria. Proceedings of the National Academy of Sciences of the United States of America. vol. 115 (34).
- (2018) Self-Coacervation of a Silk-Like Protein and Its Use As an Adhesive for Cellulosic Materials. ACS Macro Letters. vol. 7.
- (2018) Tn and STn are members of a family of carbohydrate tumor antigens that possess carbohydrate-carbohydrate interactions. Glycobiology. vol. 28 (7).
- (2017) Polymer sequencing by molecular machines: a framework for predicting the resolving power of a sliding contact force spectroscopy sequencing method. Nanoscale. vol. 9 (39).
- (2017) Interactions between the breast cancer-associated MUC1 mucins and C-type lectin characterized by optical tweezers. PLoS ONE. vol. 12 (4).
- (2016) Competitive ligand exchange of crosslinking ions for ionotropic hydrogel formation. Journal of materials chemistry. B. vol. 4 (37).
- (2016) Single molecule investigation of the onset and minimum size of the calcium-mediated junction zone in alginate. Carbohydrate Polymers. vol. 148.
- (2016) Sliding Contact Dynamic Force Spectroscopy Method for Interrogating Slow Forming Polymer Cross-Links. Langmuir. vol. 32 (48).
- (2016) Interactions of mucins with the Tn or Sialyl Tn cancer antigens including MUC1 are due to GalNAc–GalNAc interactions. Glycobiology. vol. 26 (12).
- (2016) Microarrays for the study of compartmentalized microorganisms in alginate microbeads and (W/O/W) double emulsions. RSC Advances. vol. 6.
- (2016) Versatile, cell and chip friendly method to gel alginate in microfluidic devices. Lab on a Chip. vol. 16 (19).
- (2016) Effects of added oligoguluronate on mechanical properties of Ca - Alginate - oligoguluronate hydrogels depend on chain length of the alginate. Carbohydrate Polymers. vol. 147.
- (2016) Recovering fluorophore concentration profiles from confocal images near lateral refractive index step changes. Journal of Biomedical Optics. vol. 21 (12).
- (2016) Bioresponsive DNA-co-polymer hydrogels for fabrication of sensors. Current Opinion in Colloid & Interface Science. vol. 26.
- (2016) Nanoscale Structure and Spectroscopic Probing of Aβ1-40 Fibril Bundle Formation. Frontiers in Chemistry. vol. 4.
- (2016) Nanoindentation and finite element modelling of chitosan-alginate multilayer coated hydrogels. Soft Matter. vol. 12 (35).
- (2016) Local structure of Ca2+ induced hydrogels of alginate–oligoguluronate blends determined by small-angle-X-ray scattering. Carbohydrate Polymers. vol. 152.
- (2015) Swelling Dynamics of a DNA-Polymer Hybrid Hydrogel Prepared Using Polyethylene Glycol as a Porogen. Gels. vol. 1 (2).
- (2015) Direct Determination of Chitosan–Mucin Interactions Using a Single-Molecule Strategy: Comparison to Alginate–Mucin Interactions. Polymers. vol. 7 (2).
- (2015) Single molecule study of heterotypic interactions between mucins possessing the Tn cancer antigen. Glycobiology. vol. 25 (5).