Background and activities
NMR spectrocopy on biological macromolecules
My main research interest is NMR-spectroscopy, especially NMR on biological macromolecules.
Alginate is a biological copolymer consisting of two building blocks: mannuronic acid (M) and guluronic acid (G). The properties of the polymer depend largely on the relative composition and the distribution of these two subunits. Algae and some bacteria have devised a way to turn M into G by enzymatic epimerization at C5. A family of modular type enzymes (mannuronan C5-epimerases) has identified in Azotobacter vinelandii, and these enzymes are capable of converting M to G on polymer level. The deduced epimerase sequences consist of two types of structural modules, designated A (~385 amino acids each, with 1 or 2 copies) and R (~155 amino acids each, with one to seven copies). The structure of the A-module has been solved by crystallography (PDB code: 2PYG, 2PYH). We have solved the structure of the R-module by NMR ( PDB code: 2AGM). This work is in collaboration with Reinhard Wimmer, Aalborg University, Denmark and Hideo Iwai, University of Helsinki, Finland.
Selenium and selenoproteins is known to be essential for many life forms. Selenoprotein are in general characterized as redox proteins but still their specific function and structure are largely unknown. We have determined the structure of selenoproteinW (SelW) by NMR ( PDB code: 2NPB). Furthermore, we were able to prove that SelW interact with 14-3-3 proteins. Currently, we are working on the structure and reaction mechanism of selenocystinine containing methionine-r-sulfoxide reductase (MsrB1) ( PDB code: 2KAO) and cystinine counter part MsrB2.
This work is in collaboration with Alexander Dikiy, NTNU.
Cyclodextrins and their inclusion complexes
Cyclodextrins are circulated glycosyl units with the ability to make inclusion complexes with a large variety of hydrophobic molecules. Cyclodextrins have broad application in many industrial purposes, e.g. as separation material, and as additives in food and pharmaceuticals. We try to find out more about the geometry of inclusion complexes and about the rules governing their formation. Furthermore, my main focus is the application of cyclodextrin in the field of molecular genetics and protein expression and purification.
Complex and modified sugar molecules
NOBIPOLs activity in tailoring, modification and finding new sugar molecules demand both quantitative and qualitative characterisation. A unique tool for this kind of work is NMR spectroscopy by giving atomic level structurally information and have high dynamic in its detection range.
Scientific, academic and artistic work
A selection of recent journal publications, artistic productions, books, including book and report excerpts. See all publications in the database
- (2018) Overall size of mannuronan C5-Epimerases influences their ability to epimerize modified alginates and alginate gels. Carbohydrate Polymers. vol. 180.
- (2017) Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2. Biomolecular NMR Assignments.
- (2017) Chemical shift assignments for the apo-form of the catalytic domain, the linker region, and the carbohydrate-binding domain of the cellulose-active lytic polysaccharide monooxygenase ScLPMO10C. Biomolecular NMR Assignments. vol. 11 (2).
- (2017) A novel expression system for lytic polysaccharide monooxygenases. Carbohydrate Research. vol. 448.
- (2017) Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function. Scientific Reports. vol. 7 (1).
- (2016) Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase NcLPMO9C. Biomolecular NMR Assignments. vol. 10 (2).
- (2016) Interactions of a fungal lytic polysaccharide monooxygenase with β-glucan substrates and cellobiose dehydrogenase. Proceedings of the National Academy of Sciences of the United States of America. vol. 113 (21).
- (2016) Efficient functionalization of alginate biomaterials. Biomaterials. vol. 80.
- (2016) Structural characterization of a branched (1 → 6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius. Carbohydrate Polymers. vol. 146.
- (2016) Simultaneous analysis of C1 and C4 oxidized oligosaccharides, the products of lytic polysaccharide monooxygenases acting on cellulose. Journal of Chromatography A. vol. 1445.
- (2015) The impact of chain length and flexibility in the interaction between sulfated alginates and HGF and FGF-2. Biomacromolecules. vol. 16 (11).
- (2015) 1H, 13C, 15N resonance assignment of the chitin-active lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis. Biomolecular NMR Assignments. vol. 9 (1).
- (2015) Ibuprofen-in-cyclodextrin-in-W/O/W emulsion - Improving the initial and long-term encapsulation efficiency of a model active ingredient. International Journal of Pharmaceutics. vol. 487 (1-2).
- (2015) Energy landscape of alginate-epimerase interactions assessed by optical tweezers and atomic force microscopy. PLoS ONE. vol. 10:e014237 (10).
- (2015) RGD-peptide modified alginate by a chemoenzymatic strategy for tissue engineering applications. Journal of Biomedical Materials Research. Part A. vol. 103 (3).
- (2014) Heparin-Like Properties of Sulfated Alginates with Defined Sequences and Sulfation Degrees. Biomacromolecules. vol. 15 (7).
- (2014) Structural and functional characterization of the R-modules in alginate C-5 epimerases AlgE4 and AlgE6 from Azotobacter vinelandii. Journal of Biological Chemistry. vol. 289 (45).
- (2014) The structure and regulation of human muscle α-Actinin. Cell. vol. 159 (6).
- (2014) A C4-oxidizing lytic polysaccharide monooxygenase cleaving both cellulose and cello-oligosaccharides. Journal of Biological Chemistry. vol. 289 (5).
- (2014) Isolation and characterization of glucans in Cantharellus cibarius. Planta Medica. vol. 80 (16).