Course content

This course introduces advanced theoretical concepts of chromatography, spectroscopy, and spectrometry. The course specializes in liquid-, supercritical-, and gas- chromatography for the determination of organic compounds. The course will provide an overview of sample preparation principles (e.g., solid phase extraction, SPE; and liquid-liquid extraction, LLE), chromatographic chiral separations, thin layer chromatography, electrophoresis, coupling chromatography to various detectors, and guiding principles on method validation and quantification. The course will also provide an overview of the theoretical principles of mass spectrometry (MS), infrared spectroscopy (IR), and nuclear magnetic resonance spectroscopy (NMR) since special attention is given on the analysis and interpretation of spectral data for the structural elucidation of organic compounds.

Required previous knowledge:

KJ1000, KJ1021 and KJ2050

Learning outcome

At the end of the course the student can:

- be able to explain the principles of the most important liquid and gas chromatographic as well as electro-migration techniques.

- have technical knowledge of, and some practical experience with, analyses in gas and liquid chromatography, and in capillary electrophoresis in a conceptual learning environment.

- be able to evaluate strengths and limitations of the most important chromatographic separation and detection methods in relation to the properties of the sample and of the analysis task.

- be able to consider HSE in relation to chromatographic experimentation.

- be able to explain principles of IR, MS and NMR spectroscopy.

- be able to explain construction/design of spectrometers.

- be able to analyze and interpret spectral data.

- be able to use spectral data to elucidate an unknown structure, or solve a structure-related problem.

Learning methods and activities

Lectures, lab and semester assignment. Schedule will be published at semester start.

Expected work load in the course is 200-225 hours.

As part of the course, a compulsory (course-specific) HSE lecture is included, which will take place at the start of the semester. Students who do not attend this safety lecture will not have access to the lab assignments. The students are responsible for acquiring information about the start-up time of the course and compulsory activities associated with it.

Compulsory semester assignment: structure elucidation of an unknown compound by the spectroscopic methods and theoretical exercises (ca. 20 hours).

Compulsory assignments

• Approved laboratory course
• Approved term paper

Required previous knowledge

Required previous knowledge:

• KJ2050
• Have 1 mandatory from these (organic chemistry)

HBIOA1002 Organisk kjemi

IMAK2003 Organisk kjemi og biomolekyler 2

IMAK2021 Organisk kjemi for ingeniører

KJ1024 Organisk kjemi, laboratoriekurs

TKJ4103 Organisk kjemi grunnkurs

TKJE1016 Generell og organisk kjemi

TMT4122 Generell og organisk kjemi, laboratoriekurs

• And have 1 mandatory from these (general chemistry)

HBIOA1004 Generell kjemi

IMAK1001 Generell kjemi for ingeniører

KJ1003 Generell kjemi

KJ1004 Generell kjemi

KJ1005 Generell kjemi

TKJE1006 Generell kjemi

TMT4111 Generell kjemi

Course materials

E.Lundanes, T.Greibrokk and L. Reubsaet; "Chromatography, Basic Principles, Sample Preparation and Related Methods", Wiley, 2014, ISBN 978-3-527-33620-3. Handouts given in connection with the laboratory exercises.

R.M. Silverstein, F.X. Webster, D.J. Kiemle, D.L. Bryce, Spectrometric Identification of Organic Compounds, 8th Edition, John Wiley & Sons, Inc, Danvers, MA, USA (2015)

ISBN: 978-0-470-61637-6