Rahmi Lale
About
Research
I am leading an interdisciplinary lab operating within the fields of biophysics, computational and synthetic biology. Our main focus is both understanding and controlling transcriptional and translational processes in microorganisms. We employ a range of methods, including microfluidics, single-cell arrays, and high-throughput screening. We work with a wide range of microorganisms, such as Chlamydomonas reinhardtii (chloroplast), Corynebacterium glutamicum, Escherichia coli, Pseudomonas putida KT2440, psychrophilic Pseudomonas, Psychrobacter spp., Pichia pastoris, Streptomyces albus, S. coelicolor, S. lividans, Synechococcus sp. PCC 7002, Saccharomyces cerevisiae, Vibrio natriegens and Thermus thermophilus.
Current Projects
Horizon Europe – Innovative Tools for Sustainable Exploration of Marine Microbiomes: Towards A Circular Blue Bioeconomy and Healthier Marine Environments. Project partner
RCN-FRIPRO-Norway – An interdisciplinary data-driven approach to resolve sigma-factor-specific promoter dependency in bacteria. Project leader
Finalised Projects
RCN–FORNY2020–NO – Fast-X-Press.
NTNU-Discovery – SUPERAP – fast track for efficient protein production in novel hosts
Lale Lab
For curious minds, more information can be found on our lab website on what we do: Lale Lab
Teaching
BT3220 (2018 – …) - Advanced Biotechnology
TBT4507 (2013 – …) - Bioinformatics and Statistics and Research Planning, Specialization Course
TBT4508 (2013 – …) - Bioinformatics and Biopolymeric Materials, Specialization Course
TBT4170 (2021) - Biotechnology
TBT4165 (2015 – 2018) - System Biology and Biological Networks
BI2014 (2013) - Molecular Biology
Scientific profile
Scopus
Google Scholar
PubMed
ResearchGate
ORCID
Publications
- 2022
Fages-Lartaud M, Prone W, Mueller Y, Lale R. Method for engineering artificial bidirectional promoters. Submitted (Pre-print availble at Research Square)
Lale R, Tietze L, Fages-Lartaud M, Nesje J, Onsager I, Engelhardt K, Wong CFA, Akan M, Hummel N, Kalinowski J, Rückert C, Hohmann-Marriott MF. A universal approach to gene expression engineering. OUP Synthetic Biology.
Fages-Lartaud M, Tietze L, Elie F, Lale R, Hohmann-Marriott MF. mCherry contains a fluorescent protein isoform that interferes with its reporter function. Frontiers in Bioengineering and Biotechnology - Synthetic Biology.
Tietze L, Mangold A, Hoff M, Lale R. Identification and cross-characterisation of artificial promoters and 5' untranslated regions in Vibrio natriegens. Frontiers in Bioengineering and Biotechnology - Synthetic Biology.
- 2021
Wong CFA, van Vliet L, Bhujbal SV, Guo C, Sletmoen M, Stokke BT, Hollfelder F, Lale R. A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalised Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution. ACS Synthetic Biology
Tietze L, Lale R. Importance of the 5′ Regulatory Region to Bacterial Synthetic Biology Applications. Microbial Biotechnology
- 2020
Bhujbal SV, Dekov M, Ottesen V, Dunker K, Lale R, Sletmoen M. Effect of design geometry, exposure energy, cytophilic molecules, cell type and load in fabrication of single-cell arrays using micro-contact printing. Scientific Reports.
Le SB, Onsager I, Lorentzen JA, Lale R. Dual UTR–A novel 5' untranslated region design for synthetic biology applications. OUP Synthetic Biology.
- 2019
Le SB, Onsager I, Lorentzen JA, Lale R. Dual UTR–A novel 5' untranslated region design for synthetic biology applications. Preprint at bioRxiv.
Lale R, Tietze L, Nesje J, Onsager I, Engelhardt K, Wong CFA, Akan M, Hummel N, Kalinowski J, Rückert C, Hohmann-Marriott MF. A universal method for gene expression engineering. Submitted (Preprint available at bioRxiv).
Moghadam MM, Tietze L, Wentzel A, Lale R. Biochemical Characterization of Three Laccase-Like Multicopper Oxidases from Arctic Marine Psychrobacter spp. Submitted.
- 2018
Schneider O, Simic N, Aachmann F, Rückert C, Kristiansen K, Kalinowski J, Jiang Y, Wang L, Jiang C, Lale R, Zotchev S. Genome Mining of Streptomyces sp. YIM 130001 Isolated from Lichen Affords New Thiopeptide Antibiotic. Front Microbiol.
- 2017
Ramisetty SK, Langlette P, Lale R, Dias RdS. In vitro studies of DNA condensation by bridging protein in a crowding environment. International Journal of Biological Macromolecules.
Vogel AIM, Lale R, Hohmann-Marriott MF. Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002. Journal of Biological Engineering.
Lewin A, Lale R, Wentzel A. Expression Platforms for Functional Metagenomics – Emerging technology options beyond Escherichia coli. Functional Metagenomics: Tools and Applications. Springer.
- 2016
Beal J, Haddock-Angelli T, Gershater M, de Mora K, Lizarazo M, Hollenhorst J, et al. Reproducibility of fluorescent expression from engineered biological constructs in E. coli. PLoS ONE.
Arnfinnsdottir, NB., Bjørkøy, A., Lale, R., Sletmoen, M. Heterogeneity in GFP expression in isogenic populations of P. putida KT2440 investigated using flow cytometry and bacterial microarrays. RSC Advances
Moghadam MM, Albersmeier A,Winkler A, Cimmino L, Rise K, Hohmann-Marriott FM, Kalinowski J, Rückert C, Wentzel A, Lale, R. Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity. BMC Genomics.
- 2015
Bjerga, GE, Lale, R., Williamson, A. Engineering low-temperature expression systems for heterologous expression of cold-adapted enzymes. Bioengineered
Arnfinnsdottir, NB., Ottesen, V., Lale, R., Sletmoen, M. The design of simple bacterial microarrays. Development towards immobilizing single living bacteria on predefined micro-sized spots on patterned surfaces. PLoS ONE.
- 2014
Petrov, V., Balasubramaniam, S., Lale, R., Moltchanov, D., Lio’, P., Koucheryavy, Y. Forward and Reverse Coding for Chromosome Transfer in Bacterial Nanonetworks. Nano Communication Networks.
Strand, TA., Lale, R., Degnes, KF., Lando, M. and Valla, S. A new and improved host-independent plasmid system for RK2-based conjugal transfer. PLoS ONE.
DNA Cloning and Assembly Methods. Methods in Molecular Biology, editors Rahmi Lale and Svein Valla. Volume 1116, XI, 303 p. Springer Protocols, Humana Press.
Røkke, G., Korvald, E., Pahr, J., Øyås, O., and Lale, R. BioBrick assembly standards and techniques and associated software tools. DNA Cloning and Assembly Methods. Methods in Molecular Biology, editors Lale, R. and Valla, S. Volume 1116, 2014, pp 1-24 Springer Protocols, Humana Press.
- 2013
Zwick, F., Lale, R. and Valla, S. Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter. BMC Microbiol. 13: 262
Zwick, F., Lale, R. and Valla, S. Combinatorial engineering for heterologous gene expression. Bioengineered.
Balzer, S., Kucharova, V., Megerle, J., Lale, R., Brautaset, T., Valla, S. A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli. Microbial Cell Factories, 12:26.
- 2012
Zwick, F., Lale, R. and Valla, S. Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory expression control elements in the expression cassette. Microbial Cell Factories, 11:133.
Almaas, E., Bruheim, P., Lale, R. and Valla, S. Dynamics and robustness of metabolic networks: A systems biology review of Escherichia coli metabolism. Systems Microbiology: Current Topics and Applications. ISBN: 978-1-908230-02-7.
- 2011
Aakvik, T., Lale, R., Liles, M. and Valla, S. Metagenomic libraries for functional screening, Ch 22. Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches. Ed. de Bruijn, F.J.
Lale, R., Brautaset, T.and Valla, S. Broad-host-range plasmid vectors for gene expression in bacteria. Met. Mol. Biol. 765, Part 3, 327-343.
Lale, R., Berg, L., Stüttgen, F., Netzer, R., Stafsnes, M., Brautaset, T., Aune, T.E.V. and Valla. S. Continuous control of the flow in biochemical pathways through 5'-UTR sequence modifications in mRNA expressed from the broad-host-range Pm promoter. Appl. Env. Mircobiol. 77: 2648-2655.
- 2010
Aune, T. E. V., Bakke, I., Drabløs, F., Lale, R., Brautaset, T. and Valla, S. Directed evolution of the transcription factor XylS for development of improved expression systems. Microb. Biotechnol. 3:1, 38–47.
- 2009
Berg, L., Lale, R., Bakke, I., Burroughs, N. and Valla, S. The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA-region corresponding to the 5'-untranslated part of mRNA. Microb. Biotechnol. 2(3), 379-389.
Brautaset, T., Lale, R. and Valla, S. Positively regulated bacterial expression systems. Microb. Biotechnol. 2 (1), 15-30.
- 2008
Bar, N. S. and Lale, R. Modeling and control of the protein synthesis process in eukaryotic cells. 47th IEEE Proceedings: Conference on Decision and Control (CDC) 179-184.
Competencies
Research
Publications
2022
-
Fages-Lartaud, Maxime René;
Tietze, Lisa;
Elie, Florence;
Lale, Rahmi;
Hohmann-Marriott, Martin Frank.
(2022)
mCherry contains a fluorescent protein isoform that interferes with its reporter function.
Frontiers in Bioengineering and Biotechnology.
volum 10.
Academic article
-
Tietze, Lisa;
Mangold, Antonia;
Hoff, Maria Wilhelmsen;
Lale, Rahmi.
(2022)
Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens.
Frontiers in Bioengineering and Biotechnology.
volum 10.
Academic article
2021
-
Oakes, kelly;
Lale, Rahmi.
(2021)
A new way to get cells to spill their secrets.
Norwegian SciTech News.
Popular scientific article
-
Tietze, Lisa;
Lale, Rahmi.
(2021)
Importance of the 5′ regulatory region to bacterial synthetic biology applications.
Microbial Biotechnology.
Scientific translation/review
-
Wong, Che Fai Alex;
van Vliet, Liisa;
Bhujbal, Swapnil Vilas;
Guo, Chengzhi;
Sletmoen, Marit;
Stokke, Bjørn Torger;
Hollfelder, Florian;
Lale, Rahmi.
(2021)
A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalised Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution.
ACS Synthetic Biology.
volum 10 (8).
Academic article
2020
-
Bhujbal, Swapnil Vilas;
Dekov, Maren;
Ottesen, Vegar;
Dunker, Karen;
Lale, Rahmi;
Sletmoen, Marit.
(2020)
Effect of design geometry, exposure energy, cytophilic molecules, cell type and load in fabrication of single-cell arrays using micro-contact printing.
Scientific Reports.
volum 10.
Academic article
-
Le, Simone Balzer;
Onsager, Ingerid;
Lorentzen, Jon Andreas;
Lale, Rahmi.
(2020)
Dual UTR-A novel 5′ untranslated region design for synthetic biology applications.
Synthetic Biology.
volum 5 (1).
Academic article
2019
-
Halsne, Ruth;
Lale, Rahmi.
(2019)
Bakterier med syntetisk genom.
Tidsskrift for Den norske legeforening.
Popular scientific article
-
Lale, Rahmi;
Tietze, Lisa;
Nesje, Jenny;
Onsager, Ingerid;
Engelhardt, Kerstin;
Wong, Che Fai Alex;
Akan, Madina;
Hummel, Niklas;
Kalinowski, Jörn;
Ruckert, Christian;
Hohmann-Marriott, Martin Frank.
(2019)
A universal method for gene expression engineering.
BioRxiv.
Academic article
-
Lale, Rahmi;
Wentzel, Alexander.
(2019)
Functional Metagenomics 2019 http://www.fmg2019.net/.
Web pages (information material)
2018
-
Lale, Rahmi.
(2018)
Kavliprisen i nanovitenskap.
Fra Fysikkens Verden.
Popular scientific article
-
Schneider, Olha;
Simic, Nebojsa;
Aachmann, Finn Lillelund;
Rückert, Christian;
Kristiansen, Kåre Andre;
Kalinowski, Jörn;
Jiang, Yi;
Wang, Lisong;
Jiang, Cheng-Lin;
Lale, Rahmi;
Zotchev, Sergey B.
(2018)
Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic.
Frontiers in Microbiology.
volum 9 (3139).
Academic article
2017
-
Ramisetty, Sravani Keerthi;
Langlete, Petter;
Lale, Rahmi;
Dias, Rita de Sousa.
(2017)
In vitro studies of DNA condensation by bridging protein in a crowding environment.
International Journal of Biological Macromolecules.
volum 103.
Academic article
-
Vogel, Anne Ilse Maria;
Lale, Rahmi;
Hohmann-Marriott, Martin Frank.
(2017)
Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002.
Journal of Biological Engineering.
volum 11 (19).
Academic article
2016
-
Arnfinnsdottir, Nina Bjørk;
Bjørkøy, Astrid;
Lale, Rahmi;
Sletmoen, Marit.
(2016)
Heterogeneity in GFP expression in isogenic populations of P. putida KT2440 investigated using flow cytometry and bacterial microarrays.
RSC Advances.
volum 6.
Academic article
-
Beal, J, et al.;
Lale, Rahmi.
(2016)
Reproducibility of fluorescent expression from engineered biological constructs in E. coli.
PLOS ONE.
Academic article
-
Bjerga, Gro Elin Kjæreng;
Lale, Rahmi;
Williamson, Adele Kim.
(2016)
Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes.
Bioengineered.
volum 7 (1).
Academic article
-
Shojaei Moghadam, Morteza;
Albersmeier, Andreas;
Winkler, Anika;
Cimmino, Lorenzo;
Rise, Kjersti;
Hohmann-Marriott, Martin Frank;
Kalinowski, Jörn;
Rückert, Christian;
Wentzel, Alexander;
Lale, Rahmi.
(2016)
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity.
BMC Genomics.
volum 17 (117).
Academic article
2015
-
Arnfinnsdottir, Nina Bjørk;
Ottesen, Vegar;
Lale, Rahmi;
Sletmoen, Marit.
(2015)
The Design of Simple Bacterial Microarrays: Development towards Immobilizing Single Living Bacteria on Predefined Micro-Sized Spots on Patterned Surfaces.
PLOS ONE.
volum 10 (6).
Academic article
2014
-
Aakvik, Trine;
Lale, Rahmi;
Degnes, Kristin Fløgstad;
Lando, Malin;
Valla, Svein.
(2014)
A new and improved host-independent plasmid system for RK2-based conjugal transfer.
PLOS ONE.
volum 9 (3:e90372).
Academic article
-
Lale, Rahmi;
Drejer, Eivind Bøe;
Hesthammer, Ronja;
Omtvedt, Line;
Lamb, Jacob Joseph;
Røynestad, Pål;
Augestad, Elias;
Marstrander Reehorst, Camilla;
Almaas, Eivind;
Hohmann-Marriott, Martin Frank.
(2014)
Competing in Synthetic biology.
Web pages (information material)
-
Lale, Rahmi;
Drejer, Eivind Bøe;
Hesthammer, Ronja;
Omtvedt, Line;
Lamb, Jacob Joseph;
Røynestad, Pål;
Augestad, Elias;
Marstrander Reehorst, Camilla;
Almaas, Eivind;
Hohmann-Marriott, Martin Frank.
(2014)
Only two days away from the competition.
Web pages (information material)
-
Lale, Rahmi;
Drejer, Eivind Bøe;
Hesthammer, Ronja;
Omtvedt, Line;
Lamb, Jacob Joseph;
Røynestad, Pål;
Augestad, Elias;
Reehorst, Camilla;
Almaas, Eivind;
Hohmann-Marriott, Martin Frank.
(2014)
We won the gold medal at the iGEM competiton.
Web pages (information material)
-
Petrov, Vitaly;
Balasubramaniam, Sasitharan;
Lale, Rahmi;
Moltchanov, Dmitri;
Liò, Pietro;
Koucheryavy, Yevgeni.
(2014)
Forward and Reverse coding for chromosome transfer in bacterial nanonetworks.
Nano Communication Networks.
volum 5 (1-2).
Academic article
-
Røkke, Gunvor;
Korvald, Eirin;
Pahr, Jarle;
Øyås, Ove;
Lale, Rahmi.
(2014)
BioBrick Assembly Standards and Techniques and Associated Software Tools.
DNA Cloning and Assembly Methods.
Academic chapter/article
-
Valla, Svein;
Lale, Rahmi.
(2014)
DNA Cloning and Assembly Methods.
Humana Press. 2014. ISBN 978-1-62703-763-1. Methods in molecular biology (1116).
Scientific anthology
2013
-
Balzer, Simone;
Kucharova, Veronika;
Megerle, Judith;
Lale, Rahmi;
Brautaset, Trygve;
Valla, Svein.
(2013)
A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli.
Microbial Cell Factories.
volum 12 (26).
Academic article
-
Zwick, Friederike;
Lale, Rahmi;
Valla, Svein.
(2013)
Combinatorial engineering for heterologous gene expression.
Bioengineered Bugs.
volum 4 (6).
Academic article
-
Zwick, Friederike;
Lale, Rahmi;
Valla, Svein.
(2013)
Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter.
BMC Microbiology.
volum 13.
Academic article
2012
-
Almaas, Eivind;
Bruheim, Per;
Lale, Rahmi;
Valla, Svein.
(2012)
Dynamics and robustness of metabolic networks: A systems biology review of Escherichia coli metabolism.
Systems Microbiology - Current Topics and Applications.
Academic chapter/article
-
Zwick, Friederike;
Lale, Rahmi;
Valla, Svein.
(2012)
Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette.
Microbial Cell Factories.
volum 11 (133).
Academic article
2011
-
Aakvik, Trine;
Lale, Rahmi;
Liles, Mark R.;
Valla, Svein.
(2011)
Metagenomic Libraries for Functional Screening.
Handbook of Molecular Microbial Ecology I - Metagenomics and Complementary Approaches.
Academic chapter/article
-
Lale, Rahmi;
Berg, Laila;
Stuttgen, Friederike;
Netzer, Roman;
Stafsnes, Marit Hallvardsdotter;
Brautaset, Trygve;
Aune, Trond Erik Vee;
Valla, Svein.
(2011)
Continuous Control of the Flow in Biochemical Pathways through 5 ' Untranslated Region Sequence Modifications in mRNA Expressed from the Broad-Host-Range Promoter Pm.
Applied and Environmental Microbiology.
volum 77 (8).
Academic article
-
Lale, Rahmi;
Brautaset, Trygve;
Valla, Svein.
(2011)
Broad-Host-Range Plasmid Vectors for Gene Expression in Bacteria.
Strain Engineering - Methods and Protocols, Methods in Molecular Biology.
Academic chapter/article
2010
-
Aune, Trond Erik Vee;
Bakke, Ingrid;
Drabløs, Finn;
Lale, Rahmi;
Brautaset, Trygve;
Valla, Svein.
(2010)
Directed evolution of the transcription factor XylS for development of improved expression systems.
Microbial Biotechnology.
volum 3 (1).
Academic article
2009
-
Berg, Laila;
Lale, Rahmi;
Bakke, Ingrid;
Burroughs, Nigel;
Valla, Svein.
(2009)
The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA-region corresponding to the 5'-untranslated part of mRNA.
Microbial Biotechnology.
volum 2 (3).
Academic article
-
Brautaset, Trygve;
Lale, Rahmi;
Valla, Svein.
(2009)
Minireview - Positively regulated bacterial expression systems.
Microbial Biotechnology.
volum 2 (1).
Academic article
-
Lale, Rahmi.
(2009)
The xylS/Pm expression cassette: New functional insights and application potentials.
2009. ISBN 978-82-471-1953-2.
PhD thesis
2008
-
Bar, Nadav;
Lale, Rahmi.
(2008)
Modeling and Control of the Protein Synthesis process in Eukaryotic cells.
Proceedings of the IEEE Conference on Decision & Control, including the Symposium on Adaptive Processes.
Academic article
Scientific articles
-
Fages-Lartaud, Maxime René;
Tietze, Lisa;
Elie, Florence;
Lale, Rahmi;
Hohmann-Marriott, Martin Frank.
(2022)
mCherry contains a fluorescent protein isoform that interferes with its reporter function.
Frontiers in Bioengineering and Biotechnology.
volum 10.
Academic article
-
Tietze, Lisa;
Mangold, Antonia;
Hoff, Maria Wilhelmsen;
Lale, Rahmi.
(2022)
Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens.
Frontiers in Bioengineering and Biotechnology.
volum 10.
Academic article
-
Wong, Che Fai Alex;
van Vliet, Liisa;
Bhujbal, Swapnil Vilas;
Guo, Chengzhi;
Sletmoen, Marit;
Stokke, Bjørn Torger;
Hollfelder, Florian;
Lale, Rahmi.
(2021)
A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalised Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution.
ACS Synthetic Biology.
volum 10 (8).
Academic article
-
Bhujbal, Swapnil Vilas;
Dekov, Maren;
Ottesen, Vegar;
Dunker, Karen;
Lale, Rahmi;
Sletmoen, Marit.
(2020)
Effect of design geometry, exposure energy, cytophilic molecules, cell type and load in fabrication of single-cell arrays using micro-contact printing.
Scientific Reports.
volum 10.
Academic article
-
Le, Simone Balzer;
Onsager, Ingerid;
Lorentzen, Jon Andreas;
Lale, Rahmi.
(2020)
Dual UTR-A novel 5′ untranslated region design for synthetic biology applications.
Synthetic Biology.
volum 5 (1).
Academic article
-
Lale, Rahmi;
Tietze, Lisa;
Nesje, Jenny;
Onsager, Ingerid;
Engelhardt, Kerstin;
Wong, Che Fai Alex;
Akan, Madina;
Hummel, Niklas;
Kalinowski, Jörn;
Ruckert, Christian;
Hohmann-Marriott, Martin Frank.
(2019)
A universal method for gene expression engineering.
BioRxiv.
Academic article
-
Schneider, Olha;
Simic, Nebojsa;
Aachmann, Finn Lillelund;
Rückert, Christian;
Kristiansen, Kåre Andre;
Kalinowski, Jörn;
Jiang, Yi;
Wang, Lisong;
Jiang, Cheng-Lin;
Lale, Rahmi;
Zotchev, Sergey B.
(2018)
Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic.
Frontiers in Microbiology.
volum 9 (3139).
Academic article
-
Ramisetty, Sravani Keerthi;
Langlete, Petter;
Lale, Rahmi;
Dias, Rita de Sousa.
(2017)
In vitro studies of DNA condensation by bridging protein in a crowding environment.
International Journal of Biological Macromolecules.
volum 103.
Academic article
-
Vogel, Anne Ilse Maria;
Lale, Rahmi;
Hohmann-Marriott, Martin Frank.
(2017)
Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002.
Journal of Biological Engineering.
volum 11 (19).
Academic article
-
Arnfinnsdottir, Nina Bjørk;
Bjørkøy, Astrid;
Lale, Rahmi;
Sletmoen, Marit.
(2016)
Heterogeneity in GFP expression in isogenic populations of P. putida KT2440 investigated using flow cytometry and bacterial microarrays.
RSC Advances.
volum 6.
Academic article
-
Beal, J, et al.;
Lale, Rahmi.
(2016)
Reproducibility of fluorescent expression from engineered biological constructs in E. coli.
PLOS ONE.
Academic article
-
Bjerga, Gro Elin Kjæreng;
Lale, Rahmi;
Williamson, Adele Kim.
(2016)
Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes.
Bioengineered.
volum 7 (1).
Academic article
-
Shojaei Moghadam, Morteza;
Albersmeier, Andreas;
Winkler, Anika;
Cimmino, Lorenzo;
Rise, Kjersti;
Hohmann-Marriott, Martin Frank;
Kalinowski, Jörn;
Rückert, Christian;
Wentzel, Alexander;
Lale, Rahmi.
(2016)
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity.
BMC Genomics.
volum 17 (117).
Academic article
-
Arnfinnsdottir, Nina Bjørk;
Ottesen, Vegar;
Lale, Rahmi;
Sletmoen, Marit.
(2015)
The Design of Simple Bacterial Microarrays: Development towards Immobilizing Single Living Bacteria on Predefined Micro-Sized Spots on Patterned Surfaces.
PLOS ONE.
volum 10 (6).
Academic article
-
Aakvik, Trine;
Lale, Rahmi;
Degnes, Kristin Fløgstad;
Lando, Malin;
Valla, Svein.
(2014)
A new and improved host-independent plasmid system for RK2-based conjugal transfer.
PLOS ONE.
volum 9 (3:e90372).
Academic article
-
Petrov, Vitaly;
Balasubramaniam, Sasitharan;
Lale, Rahmi;
Moltchanov, Dmitri;
Liò, Pietro;
Koucheryavy, Yevgeni.
(2014)
Forward and Reverse coding for chromosome transfer in bacterial nanonetworks.
Nano Communication Networks.
volum 5 (1-2).
Academic article
-
Balzer, Simone;
Kucharova, Veronika;
Megerle, Judith;
Lale, Rahmi;
Brautaset, Trygve;
Valla, Svein.
(2013)
A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli.
Microbial Cell Factories.
volum 12 (26).
Academic article
-
Zwick, Friederike;
Lale, Rahmi;
Valla, Svein.
(2013)
Combinatorial engineering for heterologous gene expression.
Bioengineered Bugs.
volum 4 (6).
Academic article
-
Zwick, Friederike;
Lale, Rahmi;
Valla, Svein.
(2013)
Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter.
BMC Microbiology.
volum 13.
Academic article
-
Zwick, Friederike;
Lale, Rahmi;
Valla, Svein.
(2012)
Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette.
Microbial Cell Factories.
volum 11 (133).
Academic article
-
Lale, Rahmi;
Berg, Laila;
Stuttgen, Friederike;
Netzer, Roman;
Stafsnes, Marit Hallvardsdotter;
Brautaset, Trygve;
Aune, Trond Erik Vee;
Valla, Svein.
(2011)
Continuous Control of the Flow in Biochemical Pathways through 5 ' Untranslated Region Sequence Modifications in mRNA Expressed from the Broad-Host-Range Promoter Pm.
Applied and Environmental Microbiology.
volum 77 (8).
Academic article
-
Aune, Trond Erik Vee;
Bakke, Ingrid;
Drabløs, Finn;
Lale, Rahmi;
Brautaset, Trygve;
Valla, Svein.
(2010)
Directed evolution of the transcription factor XylS for development of improved expression systems.
Microbial Biotechnology.
volum 3 (1).
Academic article
-
Berg, Laila;
Lale, Rahmi;
Bakke, Ingrid;
Burroughs, Nigel;
Valla, Svein.
(2009)
The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA-region corresponding to the 5'-untranslated part of mRNA.
Microbial Biotechnology.
volum 2 (3).
Academic article
-
Brautaset, Trygve;
Lale, Rahmi;
Valla, Svein.
(2009)
Minireview - Positively regulated bacterial expression systems.
Microbial Biotechnology.
volum 2 (1).
Academic article
-
Bar, Nadav;
Lale, Rahmi.
(2008)
Modeling and Control of the Protein Synthesis process in Eukaryotic cells.
Proceedings of the IEEE Conference on Decision & Control, including the Symposium on Adaptive Processes.
Academic article
Journal publications
-
Oakes, kelly;
Lale, Rahmi.
(2021)
A new way to get cells to spill their secrets.
Norwegian SciTech News.
Popular scientific article
-
Tietze, Lisa;
Lale, Rahmi.
(2021)
Importance of the 5′ regulatory region to bacterial synthetic biology applications.
Microbial Biotechnology.
Scientific translation/review
-
Halsne, Ruth;
Lale, Rahmi.
(2019)
Bakterier med syntetisk genom.
Tidsskrift for Den norske legeforening.
Popular scientific article
-
Lale, Rahmi.
(2018)
Kavliprisen i nanovitenskap.
Fra Fysikkens Verden.
Popular scientific article
Books
-
Valla, Svein;
Lale, Rahmi.
(2014)
DNA Cloning and Assembly Methods.
Humana Press. 2014. ISBN 978-1-62703-763-1. Methods in molecular biology (1116).
Scientific anthology
Part of book/report
-
Røkke, Gunvor;
Korvald, Eirin;
Pahr, Jarle;
Øyås, Ove;
Lale, Rahmi.
(2014)
BioBrick Assembly Standards and Techniques and Associated Software Tools.
DNA Cloning and Assembly Methods.
Academic chapter/article
-
Almaas, Eivind;
Bruheim, Per;
Lale, Rahmi;
Valla, Svein.
(2012)
Dynamics and robustness of metabolic networks: A systems biology review of Escherichia coli metabolism.
Systems Microbiology - Current Topics and Applications.
Academic chapter/article
-
Aakvik, Trine;
Lale, Rahmi;
Liles, Mark R.;
Valla, Svein.
(2011)
Metagenomic Libraries for Functional Screening.
Handbook of Molecular Microbial Ecology I - Metagenomics and Complementary Approaches.
Academic chapter/article
-
Lale, Rahmi;
Brautaset, Trygve;
Valla, Svein.
(2011)
Broad-Host-Range Plasmid Vectors for Gene Expression in Bacteria.
Strain Engineering - Methods and Protocols, Methods in Molecular Biology.
Academic chapter/article
Report
-
Lale, Rahmi.
(2009)
The xylS/Pm expression cassette: New functional insights and application potentials.
2009. ISBN 978-82-471-1953-2.
PhD thesis
Teaching
Courses
Media
2022
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PosterAhmed, Husnain; Ahsan Khan, Essa; Tietze, Lisa; Fages-Lartaud, Maxime René; Totlani, Kartik; Bjørkøy, Astrid; Stokke, Bjørn Torger; Lale, Rahmi. (2022) Matchmaking inside microdroplets: unravelling the sigma factor specific promoter binding in bacterial in-vitro transcription using droplet microfluidics. EMBL Microfluidics 2022 . EMBL; Heidelberg. 2022-07-11 - 2022-07-13.
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LectureAhmed, Husnain; Ahsan Khan, Essa; Tietze, Lisa; Fages-Lartaud, Maxime René; Totlani, Kartik; Bjørkøy, Astrid; Stokke, Bjørn Torger; Lale, Rahmi. (2022) Unravelling the sigma factor specific promoter binding in bacterial in-vitro transcription using droplet microfluidics. TNNN Conference 2022 . NTNU; Trondheim. 2022-11-30 - 2022-12-02.
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Programme participationLale, Rahmi. (2022) HSBC Build with Bio – Expert Panel: Can we really „Grow” Everything? Goals and Limits of Synthetic Biology. Online [Internett]. 2022-03-09.
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Popular scientific lectureLale, Rahmi. (2022) Lessons learned from commercializing a research driven innovation project. NORA.startup event #30: Founding an AI startup as an AI Researcher . NORA; Tromsø. 2022-04-21.
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Popular scientific lectureLale, Rahmi. (2022) Taking the leap from an AI researcher to an AI founder. NORA.startup event #30: Founding an AI startup as an AI Researcher . NORA; Tromsø. 2022-04-21.
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Popular scientific lectureTietze, Lisa; Hoff, Maria Wilhelmsen; Lale, Rahmi. (2022) Using artificial regulatory sequences to express proteins with Vibrio natriegens. NBS Contact meeting ; 2022-02-10 - 2022-02-13.
2021
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LectureLale, Rahmi. (2021) AI and Syngens. NORA.startup and NORA.EU (EU Network to Horizon Europe on AI) Event . Research Council of Norway, NORA.startup, NORA.EU; Oslo. 2021-10-21 - 2021-10-21.
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LectureLale, Rahmi. (2021) Anvendelse av felles europeisk infrastruktur for tungregning. NOKIOS 2021, Plenum 2. NOKIOS – Norsk konferanse for IKT i offentlig sektor . NTNU, Digitaliseringsdirektorat; Trondheim. 2021-10-27 - 2021-10-27.
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LectureLale, Rahmi. (2021) Gene expression optimisation. Invited lecture . ArcticZymes AS; Digital. 2021-02-19.
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InterviewLale, Rahmi; Foundation, iGEM. (2021) POV: An iGEM Judge. YouTube [Internett]. 2021-05-16.
2020
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Academic lectureLale, Rahmi. (2020) A titratable cell lysis system for intracellular products release from Escherichia coli in microfluidic droplets. Plenary lecture. 9th International Molecular Biology and Biotechnology Congress . Kafkas University; Online. 2020-12-06 - 2020-12-10.
2019
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PosterHohmann-Marriott, Martin Frank; Vogel, Anne Ilse Maria; Årstøl, Erland; Lale, Rahmi. (2019) Identification of the siderophore biosynthesis operon of Synechococcus sp. PCC 7002. 13thWorkshop on Cyanobacteria . University of Colorado-Boulder; Boulder. 2019-06-06 - 2019-06-09.
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LectureLale, Rahmi. (2019) A novel platform for generating artificial gene expression systems in bacteria and yeast. EMBO Workshop – Creating is Understanding: Synthetic Biology Masters Complexity . EMBO; Heidelberg. 2019-09-22 - 2019-09-25.
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Academic lectureLale, Rahmi. (2019) Towards rational design of promoters. Invited Presentation. Seminar . Heinrich Heine University Düsseldorf, Synthetic Microbiology; Düsseldorf. 2019-12-19 - 2019-12-19.
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PosterÅrstøl, Erland; Vogel, Anne Ilse Maria; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2019) Molecular insights into the siderophore biosynthesis of Synechococcus PCC 7002. 14th Nordic photosynthesis Congress ; 2019-05-24 - 2019-05-27.
2018
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PosterBhujbal, Swapnil Vilas; Wong, Che Fai Alex; Sletmoen, Marit; Stokke, Bjørn Torger; Lale, Rahmi. (2018) MetaFluidics: A platform for rapid and cost-effective functional metagenomic screening. Digital Life 2018 . Digital Life Norway; Bergen. 2018-03-20 - 2018-03-21.
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Academic lectureLale, Rahmi. (2018) Expanding the Synthetic Biology Toolbox. Invited presentation, DTU Biosustainability . Prof. Bernhard Ø.Palsson; DTU, Copenhagen. 2018-05-14.
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Academic lectureLale, Rahmi. (2018) Expanding the Synthetic Biology Toolbox: An overview of the tools designed at PhotoSynLab. Invited Presentation. Synthetic Biology Conference in Bremen . University of Bremen; Bremen. 2018-10-11 - 2018-10-12.
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PosterTietze, Lisa; Lale, Rahmi. (2018) The Development of a Universal Synthetic Promoter-5´ UTR Design Platform. Digital Life Norway annual conference ; 2018-03-20 - 2018-03-21.
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PosterVogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2018) A novel function of type IV pili in iron uptake in Synechococcus sp. PCC 7002. ISPR conference on microbial photosynthesis . ISPR; Vancouver. 2018-08-09 - 2018-08-12.
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PosterVogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2018) Conductive Type IV pili play an important role for iron acquisition in the marine cyanobacterium Synechococcus sp. PCC 7002. 16th International Symposium on Phototrophic Prokaryotes . International Scientific Committee of the ISPP; Vancouver. 2018-08-05 - 2018-08-09.
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PosterVogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2018) Function of type IV pili in iron acquisition in Synechococcus sp. PCC 7002. ePS-1 . ISPR/SPPS; Uppsala. 2018-06-25 - 2018-06-28.
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PosterVogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2018) Function of type IV pili in iron acquisition in Synechococcus sp. PCC 7002. ePS-Young meeting . Rui Miao and Michael Cheah; Uppsala. 2018-06-24 - 2018-06-25.
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Academic lectureVogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2018) Function of type IV pili in iron acquisition in Synechococcus sp. PCC 7002. ePS-Young meeting . Rui Miao and Michael Cheah; Uppsala. 2018-06-24.
2017
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Academic lectureLale, Rahmi. (2017) Communicating Science in the Digital Life Age. Digital Life Conference . Senter for Digital Life; Oslo. 2017-03-20 - 2017-03-21.
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Academic lectureLale, Rahmi; Valla, Svein; Balzer, Simone; Onsager, Ingerid; Lorentzen, Jon Andreas. (2017) DualUTR – A novel 5' UTR design for synthetic biology applications. Synthetic Biology UK 2017 . UK Biochemical Society; Manchester. 2017-11-27 - 2017-11-28.
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PosterVogel, Anne Ilse Maria; Lamb, Jacob Joseph; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2017) Streamlining recombination-mediated genetic engineering in Synechococcus sp. PCC 7002 by validating three neutral integration sites. SB 7.0 ; Singapore. 2017-06-13 - 2017-06-16.
2016
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PosterLamb, Jacob Joseph; Vogel, Anne Ilse Maria; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2016) The importance of pili-mediated metal acquisition in primary productivity. 2th Workshop on Cyanobacteria, Arizona State University, Tempe campus, May 19-22, 2016 ; 2016-05-19 - 2016-05-22.
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PosterVogel, Anne Ilse Maria; Lamb, Jacob Joseph; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2016) Nanowire-mediated iron acquisition in Synechococcus sp. PCC7002. 12th Workshop on Cyanobacteria, Arizona State University, Tempe campus, May 19-22, 2016 ; 2016-05-19 - 2016-05-22.
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Academic lectureLale, Rahmi. (2016) Open Science Notebook. Open Tools and Infrastructures for Biology . Ana Maria Delgado Aleman; Newcastle University. 2016-01-20 - 2016-01-21.
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PosterLamb, Jacob Joseph; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2016) Pili-mediated metal acquisition – a physiological probe for protein-mediated electron transport?. Asilomar Bioelectronics Symposium, Asilomar Conference Grounds, Pacific Grove, CA, USA, September 5-8, 2016 ; 2016-09-05 - 2016-09-08.
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PosterVogel, Anne Ilse Maria; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2016) A novel cost-efficient transformation protocol for Synechococcus sp. PCC 7002. Norwegian Plant Biology 2016, NTNU Gløshaugen Campus, Trondheim, Norway, June 15-17, 2016 ; 2016-06-15 - 2016-06-17.
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LectureVogel, Anne Ilse Maria; Lamb, Jacob Joseph; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2016) Nanowire-mediated iron acquisition in Synechococcus sp. PCC7002. 13th Nordic Photosynthesis Congress ; Copenhagen. 2016-10-25 - 2016-10-28.
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PosterVogel, Anne Ilse Maria; Lamb, Jacob Joseph; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2016) Streamlining recombineering by validating three neutral integration sites in Synechococcus sp. PCC7002. 13th Nordic Photosynthesis Congress ; Copenhagen. 2016-10-25 - 2016-10-28.
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PosterVogel, Anne Ilse Maria; Lamb, Jacob Joseph; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2016) Streamlining recombineering by validating three neutral integration sites in Synechococcus sp. PCC7002. 12th Workshop on Cyanobacteria ; Phoenix. 2016-05-19 - 2016-05-22.
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PosterVogel, Anne Ilse Maria; Lamb, Jacob Joseph; Lale, Rahmi; Hohmann-Marriott, Martin Frank. (2016) Transformation efficiency for neutral integration sites in Synechococcus sp. PCC 7002. Umeå Renewable Energy Meeting (UREM 2016), Univesity of Umeå – KBC , 23-25 February 2016 ; 2016-02-23 - 2016-02-25.
2015
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InterviewLale, Rahmi. (2015) Skaperverkstedet. Klassekampen [Avis]. 2015-08-29.
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PosterShojaei Moghadam, Morteza; Albersmeier, Andreas; Sharma, Animesh; Winkler, Anika; Cimmino, Lorenzo; Hohmann-Marriott, Martin Frank; Kalinowski, Jörn; Rückert, Christian; Wentzel, Alexander; Lale, Rahmi. (2015) Omics approaches for identification of gene candidate(s) encoding extracellular laccase like multicopper oxidase from Psychrobacter sp. P11F6 Isolated from marine arctic environment. Microbial Genomics & Metagenomics Workshop . Joint Genome Institute; 2015-09-14 - 2015-09-18.
2014
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Programme participationAlmaas, Eivind; Lale, Rahmi; Andersen, Maria; Moghaddam, Shiva; Stormo, Ellen; Adl, Patricia; Kjønstad, Ingrid Fadum. (2014) Syntetisk biologi. Schrödingers Katt [TV]. 2014-03-27.
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Academic lectureArnfinnsdottir, Nina Bjørk; Ottesen, Vegar; Lale, Rahmi; Sletmoen, Marit. (2014) Bacterial microarrays obtained by spontaneous adhesion of single bacteria on predefined spots on chemically patterned surfaces. 2014 MRS Fall Meeting & Exhibit . Materials research society (MRS); Boston. 2014-11-30 - 2014-12-05.
2013
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PosterLale, Rahmi; Ünver, Deniz; Ertesvåg, Helga; Moghadam, Morteza Shojaei; Valla, Svein. (2013) Cold-adapted (psychrophilic) marine bacteria as expression hosts. Bioprosp_13 . MABIT; Tromsø. 2013-02-20 - 2013-02-22.
2012
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PosterBalzer, Simone; Megerle, Judith; Tøndervik, Anne; Lale, Rahmi; Brautaset, Trygve; Valla, Svein. (2012) The performance of the XylS/Pm expression cassette in relation to the most commonly used systems for bacterial recombinant expression. Eighth Annual PEGS- 2012 . Cambridge Healthtech Institute; Boston, MA. 2012-04-30 - 2012-05-04.
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Academic lectureRøkke, Gunvor; Lale, Rahmi; Rowe, Alexander D.; Sletmoen, Marit. (2012) A study of G-quadruplex DNA using optical tweezers. The 4th PhD Conference in Medical Imaging . Norwegian Research School in Medical Imaging; Trondheim. 2012-11-28 - 2012-11-29.
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PosterRøkke, Gunvor; Lale, Rahmi; Stokke, Bjørn Torger; Sletmoen, Marit. (2012) Study of interaction forces between DNA and UNG using optical tweezers. Norwegian Physical Society Biophysics Meeting 2012 ; Kongsvold fjellstue. 2012-03-15 - 2012-03-17.
2011
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PosterJørgensen, Hanne; Lale, Rahmi; Valla, Svein. (2011) Use of the red fluorescent protein mCherry as a reporter gene in Pseudomonas putida to achieve higher levels of expression in the XylS/Pm regulator/promoter system. IV International Conference on Environmental, Industrial and Applied Microbiology-BioMicroWorld2011 ; Torremolinos. 2011-09-14 - 2011-09-16.
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Academic lectureLale, Rahmi. (2011) In-silico design approaches for heterologous expression in bacteria. Bioinformatics for Young Scientists 2011 ; Ullensvang, Hardanger. 2011-03-16 - 2011-03-18.
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Academic lectureLale, Rahmi; Stuttgen, Friederike; Valla, Svein. (2011) xylS/Pm expression cassette optimisation for chromosomal expression. 6th Conference on Recombinant Protein Production ; Vienna. 2011-02-16 - 2011-02-19.
2010
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Academic lectureLale, Rahmi. (2010) Abortive initiation. 1st NTNU Seminar on Nanobiotechnology and Nanomedicine ; St. Olavs/Trondheim/NTNU. 2010-02-19 - 2010-02-19.
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Academic lectureLale, Rahmi; Finnstun, Elin; Valla, Svein. (2010) Identification of Arctic Bacteria Useful for Expression of Cold-Active Enzymes. NBS Kontakmøtet 2010 ; Storefjell. 2010-01-14 - 2010-01-17.
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PosterLale, Rahmi; Valla, Svein. (2010) Broad-host-range cloning/expression vectors. The 4th International Conference on Marine Biotechnology ; Tromsø. 2010-02-24 - 2010-02-25.
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Academic lectureSekurova, Olga; Lale, Rahmi; Jørgensen, Hanne; Valla, Svein; Zotchev, Sergey. (2010) Development of new tools for heterologous expression of secondary metabolite gene clusters. 11th International Symposium on the Genetics of Industrial ; Melbourne. 2010-06-28 - 2010-07-01.
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Academic lectureSkancke, Jørgen; Lale, Rahmi; Bar, Nadav. (2010) Free Energy Caculations Give Insight Into The Process of Promoter Escape. 11th International Conference on Systems Biology ; Edinburgh, Scotland. 2010-10-11 - 2010-10-14.
2009
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Academic lectureLale, Rahmi; Stuttgen, Friederike; Bakke, Ingrid; Valla, Svein. (2009) A comprehensive combinatorial approach to achieve high-level heterologous protein expression by chromosomal integration of a single gene copy in Escherichia coli. NBS Kontakmøtet 2009 ; Røros. 2009-01-29 - 2009-02-01.
2008
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Academic lectureLale, Rahmi; Berg, Laila; Bakke, Ingrid; Burroughs, N.; Valla, Svein. (2008) 5'-untranslated region’s role in transcription. 5th Congress on Recombinant Protein Production ; Sardinia. 2008-09-24 - 2008-09-28.