Background and activities
I am working as an Assoc. Prof. / senior researcher at the Department of Biotechnology, NTNU. My main research activities are within computational and synthetic biology, and biophysics. In our lab, we employ various methods, such as microfluidics, single-cell arrays, and high-throughput screening. We work with a large selection of microorganisms including Corynebacterium glutamicum, Chlamydomonas reinhardtii (chloroplast), Escherichia coli, Pseudomonas putida KT2440, psychrophilic Pseudomonas, Psychrobacter spp., Pichia pastoris, Streptomyces albus, S. coelicolor, S. lividans, Synechococcus sp. PCC 7002, Saccharomyces cerevisiae, Vibrio natriegens and Thermus thermophilus.
RCN-FRIPRO-Norway – An interdisciplinary data-driven approach to resolve sigma-factor-specific promoter dependency in bacteria.
RCN–FORNY2020–NO – Fast-X-Press
NTNU-Discovery – SUPERAP – fast track for efficient protein production in novel hosts
For curious minds, more information can be found on our lab website on what we do: Lale Lab
TBT4170 – Biotechnology
TBT4507 - Bioinformatics and Statistics and Research Planning, Specialization Course
BT3220 – Advanced Biotechnology
Wong CFA, Bhujbal SV, van Vliet L, Guo C, Sletmoen M, Stokke BT, Hollfelder F, Lale R. A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalised Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution. Submitted
Tietze L, Lale R. 5′ Regulatory Region Engineering Strategies for Bacterial Synthetic Biology Applications. Submitted
Bhujbal SV, Dekov M, Ottesen V, Dunker K, Lale R, Sletmoen M. Effect of design geometry, exposure energy, cytophilic molecules, cell type and load in fabrication of single-cell arrays using micro-contact printing. Scientific Reports.
Le SB, Onsager I, Lorentzen JA, Lale R. Dual UTR–A novel 5' untranslated region design for synthetic biology applications. Synthetic Biology.
Le SB, Onsager I, Lorentzen JA, Lale R. Dual UTR–A novel 5' untranslated region design for synthetic biology applications. Preprint at bioRxiv.
Lale R, Tietze L, Nesje J, Onsager I, Engelhardt K, Wong CFA, Akan M, Hummel N, Kalinowski J, Rückert C, Hohmann-Marriott MF. A universal method for gene expression engineering. Submitted (Preprint available at bioRxiv).
Moghadam MM, Tietze L, Wentzel A, Lale R. Biochemical Characterization of Three Laccase-Like Multicopper Oxidases from Arctic Marine Psychrobacter spp. Submitted.
Schneider O, Simic N, Aachmann F, Rückert C, Kristiansen K, Kalinowski J, Jiang Y, Wang L, Jiang C, Lale R, Zotchev S. Genome Mining of Streptomyces sp. YIM 130001 Isolated from Lichen Affords New Thiopeptide Antibiotic. Front Microbiol.
Ramisetty SK, Langlette P, Lale R, Dias RdS. In vitro studies of DNA condensation by bridging protein in a crowding environment. International Journal of Biological Macromolecules.
Vogel AIM, Lale R, Hohmann-Marriott MF. Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002. Journal of Biological Engineering.
Lewin A, Lale R, Wentzel A. Expression Platforms for Functional Metagenomics – Emerging technology options beyond Escherichia coli. Functional Metagenomics: Tools and Applications. Springer.
Beal J, Haddock-Angelli T, Gershater M, de Mora K, Lizarazo M, Hollenhorst J, et al. Reproducibility of fluorescent expression from engineered biological constructs in E. coli. PLoS ONE.
Arnfinnsdottir, NB., Bjørkøy, A., Lale, R., Sletmoen, M. Heterogeneity in GFP expression in isogenic populations of P. putida KT2440 investigated using flow cytometry and bacterial microarrays. RSC Advances
Moghadam MM, Albersmeier A,Winkler A, Cimmino L, Rise K, Hohmann-Marriott FM, Kalinowski J, Rückert C, Wentzel A, Lale, R. Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity. BMC Genomics.
Bjerga, GE, Lale, R., Williamson, A. Engineering low-temperature expression systems for heterologous expression of cold-adapted enzymes. Bioengineered
Arnfinnsdottir, NB., Ottesen, V., Lale, R., Sletmoen, M. The design of simple bacterial microarrays. Development towards immobilizing single living bacteria on predefined micro-sized spots on patterned surfaces. PLoS ONE.
Petrov, V., Balasubramaniam, S., Lale, R., Moltchanov, D., Lio’, P., Koucheryavy, Y. Forward and Reverse Coding for Chromosome Transfer in Bacterial Nanonetworks. Nano Communication Networks.
Strand, TA., Lale, R., Degnes, KF., Lando, M. and Valla, S. A new and improved host-independent plasmid system for RK2-based conjugal transfer. PLoS ONE.
DNA Cloning and Assembly Methods. Methods in Molecular Biology, editors Rahmi Lale and Svein Valla. Volume 1116, XI, 303 p. Springer Protocols, Humana Press.
Røkke, G., Korvald, E., Pahr, J., Øyås, O., and Lale, R. BioBrick assembly standards and techniques and associated software tools. DNA Cloning and Assembly Methods. Methods in Molecular Biology, editors Lale, R. and Valla, S. Volume 1116, 2014, pp 1-24 Springer Protocols, Humana Press.
Zwick, F., Lale, R. and Valla, S. Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter. BMC Microbiol. 13: 262
Zwick, F., Lale, R. and Valla, S. Combinatorial engineering for heterologous gene expression. Bioengineered.
Balzer, S., Kucharova, V., Megerle, J., Lale, R., Brautaset, T., Valla, S. A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli. Microbial Cell Factories, 12:26.
Zwick, F., Lale, R. and Valla, S. Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory expression control elements in the expression cassette. Microbial Cell Factories, 11:133.
Almaas, E., Bruheim, P., Lale, R. and Valla, S. Dynamics and robustness of metabolic networks: A systems biology review of Escherichia coli metabolism. Systems Microbiology: Current Topics and Applications. ISBN: 978-1-908230-02-7.
Aakvik, T., Lale, R., Liles, M. and Valla, S. Metagenomic libraries for functional screening, Ch 22. Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches. Ed. de Bruijn, F.J.
Lale, R., Brautaset, T.and Valla, S. Broad-host-range plasmid vectors for gene expression in bacteria. Met. Mol. Biol. 765, Part 3, 327-343.
Lale, R., Berg, L., Stüttgen, F., Netzer, R., Stafsnes, M., Brautaset, T., Aune, T.E.V. and Valla. S. Continuous control of the flow in biochemical pathways through 5'-UTR sequence modifications in mRNA expressed from the broad-host-range Pm promoter. Appl. Env. Mircobiol. 77: 2648-2655.
Aune, T. E. V., Bakke, I., Drabløs, F., Lale, R., Brautaset, T. and Valla, S. Directed evolution of the transcription factor XylS for development of improved expression systems. Microb. Biotechnol. 3:1, 38–47.
Berg, L., Lale, R., Bakke, I., Burroughs, N. and Valla, S. The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA-region corresponding to the 5'-untranslated part of mRNA. Microb. Biotechnol. 2(3), 379-389.
Brautaset, T., Lale, R. and Valla, S. Positively regulated bacterial expression systems. Microb. Biotechnol. 2 (1), 15-30.
Bar, N. S. and Lale, R. Modeling and control of the protein synthesis process in eukaryotic cells. 47th IEEE Proceedings: Conference on Decision and Control (CDC) 179-184.