Course content

The course includes relevant laboratory techniques that are put together into comprehensive exercises. Methods such as end-point PCR, real-time PCR, bacterial genotyping and DNA isolation are combined into an exercise in which genetic composition of microgranisms in the environment is investigated. Furthermore, RNA isolation, cell culture, cDNA synthesis, qPCR, flow cytometry and protein analysis are combined to look at cellular stress. Stress analyzes are performed using several methods such as ELISA and HPLC to look at different methods for measuring the same analyte. The course concludes with a process and analysis where, among other things, fermentation, analysis of lipids / proteins / carbohydrates gives a little insight into industrial biotechnology.

Learning outcome

Læringsutbytte - Kunnskap:

The students should have

knowledge on biotechnological analyziz and the utilization of these

knowledge about procedures and utilization of such

Knowledge to combine biotechnological methods to obtain analytical results 

    

Læringsutbytte - Ferdigheter:

The students should be able to

work with biotechnology analyzis on a Laboratory

utilize standard procedures for biotechnological methods

work with diffrent biotechnological materials

    

Læringsutbytte - Kompetanse:

basic knowledge about modern biotechnological methods applied.

knowledge about modern biotechnological methods for extraction and analyzing nucleic acids, protein and organic lipids, particulary marine lipids.

the ability to assess the ethical aspects of using biotechnological methods.

Learning methods and activities

Pedagogiske metoder:

Compulsory laboratory exercises     

Obligatoriske arbeidskrav:

Up to 15 lab days of 8 hours, with associated journal book must be approved before the student is allowed to sit for the exam. The journal book is approved after completion of the lab course. Estimated study time for the course is 400 hours, of which 150 are organized laboratory courses and lectures, and 100 hours are expected for preparation for the laboratory course

Compulsory assignments

• Mandatory assignment

Specific conditions

Required previous knowledge

MB101812 General Microbiology, MK 101309 Organic chemistry and biochemistry and BI200115 Cell biologi with molecular biology or corresponding courses.

Credit reductions