Intracellular recording labeling

Department of Psychology

Intracellular recording labeling

– Chemosensory laboratory

Intracellular recording/labeling and integration of a neuronal models into a standard brain atlas:

Morphological and physiological characteristics of individual neurons are possible to obtain via intracellular recordings coupled with iontophoretic labeling. This method, though technically quite demanding, provide precise findings on the basic computational unit in the brain, the neuron. Together, this kind of data give a basis for further classification of the neurons, which is absolutely necessary for achieving a deeper understanding of a neural circuit’s functional specificity. Our studies on antennal-lobe projection neurons have demonstrated a high morphological diversity, particularly regarding lateral-tract neurons. Yet even this variety is likely only a part of the real neuronal repertoire indicating the morphological complexity typifying the olfactory pathways in the small insect brain.

Further, high quality raw data images obtained via confocal microscopy are used for digital reconstruction and subsequent registration of the neurons within a common brain framework. This gives us a 3D visualization of the neuronal elements and the possibility to compare their output areas. These kind of data are also presented in the database Insect brain database, an international web site that offers a direct access to a growing databank of insect neurons, presenting their functional and anatomical diversity. 

Lab members

Lab members

  • Xi Chu, Research fellow
  • Elena Ian, Research fellow
  • Pramod KC, PhD candidate
  • Jonas Kymre, PhD candidate
  • Ina Saltvik, master student
  • Marte Schetne, master student
  • Line Aune, master student
  • Rikke I. Henriksen, master student
  • Sara Holm, master student
  • Hanna Mustaparta, Professor emeritus