Transcriptomics - K.G. Jebsen Center for Genetic Epidemiology
Through HUNT Biobank we have access to cryopreserved blood cells from about 40.000 individuals includes in HUNT3. This resource opens new possibilities to perform single cell gene expression analysis by RNA-seq to measure cell type-specific expression profiles on the same previously genotyped individuals. We expect that this deep molecular phenotyping will reveal genetic variants predictive of individual variation in cell type composition and cell type-specific gene expression. We will complement the blood-based analyses with transcriptome analyses of tissue from psoriasis patients.
Psoriasis is a chronic inflammatory skin disease where the skin lesion development is closely tied to an extensive cytokine network. By combining blood eQTLs and tissue expression we will determine to what extent disease-associated genetic variants and expression differences between psoriatic-related and normal skin are reflected in blood composition or gene expression.