Proteomics Analyses

Composite image illustrating proteomics workflows, including a protein structure model, LC-MS/MS chromatograms and spectra, quantitative analysis plots, and a protein expression heat map.

 

Samples

We analyze proteins and peptides in a wide range of sample types and states. Samples may be provided in solution, dried, or as gel bands. We can process and analyze cell cultures, tissues (including FFPE samples), biological fluids, and other materials (for example, paint shavings).

Wetlab Services

  • Purification of proteins and peptides.
  • Separation by 1D/2D-PAGE, 1D/2D Western blotting, capillary Western analysis, and OFFGEL isoelectric focusing.
  • Preparation of samples for LC-MS/MS analysis.
  • Enrichment of post-translational modifications (PTMs).​​​​

Analyses

Our LC-MS/MS-based analyses encompass:

  • Protein identification
  • Protein quantification
  • Analysis of post-translational modifications, primarily phosphorylation and ubiquitination
  • Intact protein analysis
  • Protein interaction studies
  • De novo sequencing

Practical Information

We encourage you to contact us at an early stage when planning your proteomics project, even before applying for funding. We can help estimate costs and provide guidance on incorporating proteomics analyses into your funding application.

We can also advise on experimental design and sample preparation. It is particularly important to contact us if your project involves the identification or quantification of challenging proteins, such as low-abundance proteins, heavily modified proteins, or highly hydrophobic proteins. We can provide guidance on sample preparation strategies to maximize the likelihood of achieving your research objectives.