Phytohormone quantification

 

One of the applications the metabolomics facility is routinely used for is the quantification of plant phytohormones like jasmonic and salicylic acid that are important signaling molecules mediating stress response in plants. The method has been originally developed by Forcat and colleagues (Forcat et al., 2008 doi:10.1186/1746-4811-4-16), uses  2H2 JA  and 2H4 SA labeled standards for quantification purposes and has been simplified by us to increase throughput.

Graph

 

Thorsten Hamann, 2013