TAMiR - Targeting antimicrobial resistance by inhibition of bacterial stress responses - Department of Clinical and Molecular Medicine
Research Topics and Publications
Research Topics and Publications
Our lead peptide, the APIM-peptide, targets DNA sliding clamps. DNA sliding clamps are functionally and structurally conserved in all three domains of life. The bacterial DNA sliding clamp, the β-clamp, is essential for DNA replication and translesion synthesis (TLS). The APIM-peptide binds to the β-clamp and blocks protein interactions important for the execution of these processes. The APIM-peptide efficiently reduces the growth of both gram positive and gram negative bacterial strains as well as biofilm forming and multidrug-resistant bacteria.
We have demonstrated in vivo effects in an MRSA skin infection model and an MRSE bone graft infection model where the APIM-peptide was applied as a gel or in cement, respectively. Importantly, bacteria are killed at doses that are not toxic to human cells, supporting it´s potential for clinical development as an antibiotic. Additionally, the APIM-peptide strongly inhibits mutagenesis, and these anti-mutagenic properties of the APIM-peptide could be important to inhibit the development of antimicrobial resistance when combined with other antibiotics.
We are currently focusing on optimizing the APIM-peptide for increased affinity to the β-clamp, elucidating its mode of action in bacterial stress responses and on selecting a clinical indication for further development of the APIM-peptide as a new “antibiotics”.
- Nucleoside ANalogues Are Potent Inducers of Pol V-mediated Mutagenesis, Biomolecules, June 2021.
- Novel Peptides Targeting the β-Clamp Rapidly Kill Planktonic and Biofilm Staphylococcus epidermidis Both in vitro and in vivo, Frontiers in Microbiology, 17 March 2021
- Peptides containing the PCNA interacting motif APIM bind to the beta-clamp and inhibit bacterial growth and mutagenesis, Nucleic Acids Research, 04 June 2020
The project on the APIM-peptide is led by Professor Marit Otterlei
The toxin-antitoxin (TA) systems are widely distributed in bacteria and consist of small genetic elements; a stable toxin and its unstable neutralizing conjugate antitoxin. During stressful conditions, the antitoxin is degraded, leaving free active toxin. Toxins can interfere with essential cellular processes such as DNA replication, translation and cell wall synthesis, and enable bacteria to survive stress by inducing bacterial persistence, biofilm formation and antibiotic tolerance.
However, overexpression of toxins can be lethal to the bacterial cell, and this property can be exploited for antimicrobial purposes. We are currently studying the mode of action and antimicrobial effects of peptides derived from type I TA systems, which interact with the inner membrane and have the potential for development into antimicrobial therapeutics.
The TA-peptides project is led by Professor Magnar Bjørås
We are screening for small molecule peptide mimickers that have high affinity for the β-clamp/peptide complex in an in silico virtual screen using a crystallized β-clamp protein and about 5 million commercially available compounds and drug-like molecules.
The most potent candidates will be further studied for potential use as antibiotics.
- Norske forskere med nytt antibiotika-funn, NRK Vestland, 21.02.2019
- NRK Radio, Ekko samfunnspodden, 26.03.2019
- Norske forskere med oppsiktsvekkende oppdagelse – kan ha funnet ny type antibiotika, NRK Trøndelag 24.06.2020
- NRK Radio Ukeslutt, 27.06.2020
AMR-bridge is a consortium for the four AMR-projects in Norway founded by Trond Mohn Foundation. AMR-bridge arrange an annual meeting in order to increase the awareness of the AMR-problem, underline the importance of research and promote research collaboration. The TAMiR project hosted the AMR-bridge annual meeting 2020 addressing "The need for new antibiotics" at Jægtvolden October 13th-14th.